References of "Hanikenne, Marc"
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See detailWhole Plant Phenotyping and Molecular Identification of Zn Transporters During Zn Deficiency and Excess in Brachypodium distachyon
Amini, Sahand ULiege; Arsova, Borjana; Watt, Michelle et al

Poster (2019, June)

Zinc (Zn) is an essential micronutrient for plants and around two billion people are depending on legumes and grains as their main Zn source. This transition metal is however toxic for plants at high ... [more ▼]

Zinc (Zn) is an essential micronutrient for plants and around two billion people are depending on legumes and grains as their main Zn source. This transition metal is however toxic for plants at high concentrations in soils and affects biomass and yield. The eudicot model plant Arabidopsis thaliana has already been characterized for Zn homeostasis under various Zn regimes and recently we have investigated the transcriptome, proteome and ionome upon Zn deficiency and re-supply in this species (Arsova, B. et al., 2019, bioRxiv, 600569). Although some important players of Zn uptake and transport, such as ZRT, IRT-like Protein (ZIP) family genes, have been studied in wheat, rice and barley, it is unclear how different or similar are the responses of monocots and dicots to Zn supply at the molecular level. In the current study, we aim at characterizing Zn regulation in the model plant Brachypodium distachyon. For this, 10-day-old Brachypodium plants were treated with high- and low-Zn medium for two and three weeks. We measured root length, shoot weight and leaf area to examine how Zn supply influences Brachypodium morphology. Inductively coupled plasma atomic emission spectroscopy (ICP-AES) analysis was performed to determine Zn and other metal ion distribution in root and shoot under different Zn treatments. Quantitative RT-PCR analysis of 20 genes orthologous to Arabidopsis Zn homeostasis genes revealed their expression pattern in response to Zn depletion and surplus. We observed the most commonality between Brachypodium and Arabidopsis, as well as the highest correlation between Zn content and gene regulation, for ZIP genes. In general, our results show that comparative molecular study can be useful to reveal the Zn regulatory mechanisms in monocots, which can lead to new Zn biofortification and stress resistance strategies in vital grains. [less ▲]

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See detailZinc excess and the cell cycle control in Arabidopsis sp.
Thiébaut, Noémie ULiege; Zarattini, Marco; Verbruggen, Nathalie et al

Poster (2019, February 07)

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See detailHomology modeling and in vivo functional characterization of the zinc permeation pathway in a heavy metal P-type ATPase
Lekeux, Gilles ULiege; Crowet, Jean-Marc; Nouet, Cécile ULiege et al

in Journal of Experimental Botany (2019), 70

The P1B ATPase Heavy Metal ATPase 4 (HMA4) is responsible for zinc and cadmium translocation from roots to shoots in the plant Arabidopsis thaliana. It couples ATP hydrolysis to cytosolic domain movements ... [more ▼]

The P1B ATPase Heavy Metal ATPase 4 (HMA4) is responsible for zinc and cadmium translocation from roots to shoots in the plant Arabidopsis thaliana. It couples ATP hydrolysis to cytosolic domain movements enabling metal transport across the membrane. Thanks to high conservation level within the P-type ATPase family, the role of the HMA4 cytoplasmic catalytic domains can be inferred from well characterized pumps. In contrast, the function of its terminal cytosolic extensions as well as the metal permeation mechanism through the membrane remains elusive. Here, homology modeling of the HMA4 transmembrane region was conducted based on the crystal structure of a ZntA bacterial homolog. The analysis highlighted amino acids forming a metal permeation pathway, whose importance was subsequently investigated functionally through mutagenesis and complementation experiments in plants. Although the zinc pathway displayed overall conservation among the two proteins, significant differences were observed, especially in the entrance area with altered electronegativity and the presence of a salt bridge/H-bond network. The analysis also newly identified amino acids whose mutation results in total or partial loss of the protein function. In addition, comparison of zinc and cadmium accumulation in shoots of A. thaliana complemented lines revealed a number of HMA4 mutants exhibiting different abilities in zinc and cadmium translocation. These observations could be instrumental to design low cadmium accumulating crops, hence decreasing human cadmium exposure . [less ▲]

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See detailMicrobial diversity in the rhizosphere of plant metal hyperaccumulators
Bertrand, Amandine ULiege; Detry, Emilie ULiege; Nouet, Cécile ULiege et al

Conference (2018, November 08)

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See detailThe MechaGro project
Thiébaut, Noémie ULiege; Hanikenne, Marc ULiege

Conference (2018, October)

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See detailThe GPR1/FUN34/YaaH(/SatP) members in Chlamydomonas reinhardtii, are they novel peroxisomal transporters?
Durante, Lorenzo ULiege; Lauersen, Kyle J.; Hübner, Wolfgang et al

Poster (2018, June 17)

The green microalga C. reinhardtii can grow heterotrophically in presence of acetate in the dark as sole carbon source, due to the anaplerotic pathway of glyoxylate cycle1 almost completely localised in ... [more ▼]

The green microalga C. reinhardtii can grow heterotrophically in presence of acetate in the dark as sole carbon source, due to the anaplerotic pathway of glyoxylate cycle1 almost completely localised in the peroxisomal microbodies2 . Despite the significant advances gained on carbon metabolism, the genes involved in acetate transport are not yet identified. Recently, five functionally uncharacterized members predicted to have transporter-like structure were classified within the protein family GPR1/FUN34/YaaH(/SatP) (GFY)3, which comprises genes coding for putative carboxylic acids transporters in other microorganisms, including acetate as substrate4. In C. reinhardtii, GFY1-5 as we shown by YFP fusion protein, localise to peroxisomal membranes. In addition, we also showed that these genes present distinct expression patterns in different cultivation conditions, which correlates with associated co-expressed genes found in the databases. Therefore, although these proteins share between 78 and 97% similarity between their amino acid sequences, this might suggest non-redundant roles. To gain insight on their role, we are currently analysing insertional and knock-down mutants. Mutants for the genes GFY1, GFY2 and GFY3 were obtained from the Chlamydomonas Library Project 5. Mutants for GFY4, GFY5 and all GFY genes are presently under generation via artificial micro RNA. In conclusion, GFY members could represent different protagonists in transporting substrates in/out of peroxisomal microbodies, as putative transporters/permeases function. [less ▲]

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See detailThe knotty phenotype of the frd3 Arabidopsis mutant
Scheepers, Maxime ULiege; Spielmann, Julien ULiege; Boulanger, Madeleine ULiege et al

Poster (2018, May 17)

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See detailComplete Genome Sequence of Streptomyces lunaelactis MM109T, Isolated from Cave Moonmilk Deposits
Naomé, Aymeric ULiege; Maciejewska, Marta; Calusinska, Magdalena et al

in Genome Announcements (2018), 6(21), 00435-18

Detailed reference viewed: 53 (8 ULiège)
See detailThe knotty phenotype of the frd3 Arabidopsis mutant
Scheepers, Maxime ULiege; Spielmann, Julien ULiege; Boulanger, Madeleine ULiege et al

Poster (2018, February 01)

Detailed reference viewed: 24 (3 ULiège)
See detailWhen splicing meets environmental challenges
Fanara, Steven ULiege; Hanikenne, Marc ULiege; Motte, Patrick ULiege

Conference (2018, February 01)

Detailed reference viewed: 18 (5 ULiège)