References of "Hanikenne, Marc"
     in
Bookmark and Share    
Full Text
See detailRoot Apical Meristem ionic and transcriptomic response to Zinc excess stress
Thiébaut, Noémie ULiege; Richtmann, Ludwig; Ranjan, Alok et al

Poster (2021, May 26)

Exploitation of previously industrialized soils is a challenge complicated by the presence of pollutants left by different activities. Among these pollutants, high metallic concentration is strongly ... [more ▼]

Exploitation of previously industrialized soils is a challenge complicated by the presence of pollutants left by different activities. Among these pollutants, high metallic concentration is strongly affecting the plants growth, and limits the possibilities of site exploitation for agricultural or even ecological purpose. One promising solution for valorization of the soils is phytoremediation. Several species, classed as hyper-accumulative plants, can grow on these polluted soils and actively extract toxic metals and store them in their aerial parts. For instance, some Arabidopsis halleri populations accumulate zinc in its aerial parts up to a level that would be toxic to any other Arabidopsis spp. Under normal conditions, zinc presence in soil is not detrimental for plants, as it is a necessary micronutrient. However, in these polluted soils, its concentration is often excessive and phytotoxic. Absorption starts in the root system and this system’s length will directly limit the depth of the possible phytoremediation. The primary root growth depends on root tip’s homeostasis, which is regulated by hormones balance, and ROS balance. Zinc excess has been shown to have an effect on auxin production and induce ROS in diverse conditions and species. Moreover, it could have a direct effect on cell cycle phases and on other unexpected processes, as zinc is an essential cofactor to many metalloenzymes, zinc-finger transcription factors and F-box containing proteins. Therefore, Arabidopsis root tips are ideal candidates to further study zinc concentration variation responses. Firstly, a description of the effect on the meristem size and root tip organization has been done. Secondly, an assessment of the zinc concentration in the root tip was conducted by ICP-MS to hypothesize if there is an active exclusion from this mitotically active part. Finally, this question and others were further addressed by a description and comparison between a classical and apical root transcriptomic response. By working with the model species Arabidopsis thaliana, we hope to set the bases to understand the metallic stress’ effect on root growth and physiology. We focus on unravelling the exact physiological effect of zinc on the root tip organization, ionic composition and transcriptomic profile. [less ▲]

Detailed reference viewed: 47 (3 ULiège)
See detailA MAGIC desing to study mineral nutrition in Chlamydomonas reinhardtii
Cardoso Esteves, Sara Marina ULiege; Iacono, Fabrizio ULiege; Jadoul, Alice ULiege et al

Poster (2021, March)

Natural trait variation is present across all domains of life. Individuals of the same species often present differences in response to environmental stress or in adaptation. Some traits, such as biomass ... [more ▼]

Natural trait variation is present across all domains of life. Individuals of the same species often present differences in response to environmental stress or in adaptation. Some traits, such as biomass production, have economical interest in the industrial, pharmaceutical or agricultural sectors. Identifying the genetic variants affecting these processes is, therefore, fundamental. Chlamydomonas reinhardtii is the reference unicellular photosynthetic eukaryote model organism. This haploid unicellular green alga is capable of sexual reproduction, has a high recombination rate and a very short generation interval. Furthermore, C. reinhardtii can metabolize alternative carbon sources and grow under nutrient deficiency. Studying its mineral nutrition is essential to understand adaptation to the environment. A Multiparent Advanced Generation Inter-Cross (MAGIC) design is used to study genetic variation. Eight founder lines presenting phenotypic variation were intercrossed in a determined design where each founder line contributes equally, for 8 generations. A total of 768 terminal lines, consisting of a genetic mosaic of the founder lines, were obtained. Mineral nutrition of the terminal lines is explored using mixotrophy as control condition, and 10 conditions were tested: autotrophy, 5 macronutrient deprivation, and 4 micronutrient deprivation. Cells are pre-cultured in control media for 2 days, transferred to treatment media, then harvested after 4 days. Phenotypic variation in the terminal lines of the MAGIC design largely exceeds the variability observed in founder lines, indicating that new diversity has been obtained. Together with genome sequencing of the terminal lines, the dataset will enable Quantitative Trait Loci mapping for mineral nutrition in C. reinhardtii. Financing: This project is funded by a University of Liège fellowship under the ARC project Green MAGIC [less ▲]

Detailed reference viewed: 52 (6 ULiège)
Full Text
See detailAltered metal distribution in the sr45-1 Arabidopsis mutant causes developmental defects
Fanara, Steven ULiege; Schloesser, M.; Hanikenne, Marc ULiege et al

in bioRxiv (2021)

The plant SR (serine/arginine-rich) splicing factor SR45 plays important roles in several biological processes, such as splicing, DNA methylation, innate immunity, glucose regulation and ABA signaling. A ... [more ▼]

The plant SR (serine/arginine-rich) splicing factor SR45 plays important roles in several biological processes, such as splicing, DNA methylation, innate immunity, glucose regulation and ABA signaling. A homozygous Arabidopsis sr45-1 null mutant is viable, but exhibits diverse phenotypic alterations, including delayed root development, late flowering, shorter siliques with fewer seeds, narrower leaves and petals, and unusual numbers of floral organs. Here, we report that the sr45-1 mutant presents an unexpected constitutive iron deficiency phenotype characterized by altered metal distribution in the plant. RNA-Sequencing highlighted severe perturbations in metal homeostasis, phenylpropanoid pathway, oxidative stress responses, and reproductive development. Ionomic quantification and histochemical staining revealed strong iron accumulation in the sr45-1 root tissues accompanied by an iron starvation in aerial parts. We showed that some sr45-1 developmental abnormalities can be complemented by exogenous iron supply. Our findings provide new insight into the molecular mechanisms governing the phenotypes of the sr45-1 mutant. The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission. [less ▲]

Detailed reference viewed: 20 (3 ULiège)
Full Text
Peer Reviewed
See detailAdaptation of Arabidopsis halleri to extreme metal pollution through limited metal accumulation involves changes in cell wall composition and metal homeostasis.
Corso, Massimiliano; An, Xinhui; Jones, Catherine Yvonne et al

in New Phytologist (2021), 230

Metallophytes constitute powerful models to study metal homeostasis, adaptation to extreme environments and the evolution of naturally-selected traits. Arabidopsis halleri is a pseudometallophyte which ... [more ▼]

Metallophytes constitute powerful models to study metal homeostasis, adaptation to extreme environments and the evolution of naturally-selected traits. Arabidopsis halleri is a pseudometallophyte which shows constitutive Zn/Cd tolerance and Zn hyperaccumulation but high intraspecific variability in Cd accumulation. To examine the molecular basis of the variation in metal tolerance and accumulation, ionome, transcriptome and cell-wall glycan array profiles were compared in two genetically close A. halleri populations from metalliferous and non-metalliferous sites in Northern Italy. The metallicolous population displayed increased tolerance to, reduced hyperaccumulation of Zn and limited accumulation of Cd, as well as altered metal homeostasis, compared to the non-metallicolous population. This correlated well with the differential expression of transporter genes involved in trace metal entry and in Cd/Zn vacuolar sequestration in roots. Many cell wall-related genes were also more expressed in roots of the metallicolous population. Glycan array and histological staining analyses supported major differences between the two populations in the accumulation of specific root pectins and hemicelluloses epitopes. Our results supported a role for specific cell wall components and regulation of transporter genes of Arabidopsis halleri in limiting accumulation of metals on contaminated sites. [less ▲]

Detailed reference viewed: 37 (7 ULiège)
Full Text
Peer Reviewed
See detailCoordinated homeostasis of essential mineral nutrients: a focus on iron.
Hanikenne, Marc ULiege; Cardoso Esteves, Sara Marina ULiege; Fanara, Steven ULiege et al

in Journal of experimental botany (2021), 72

In plants, iron (Fe) transport and homeostasis are highly regulated processes. Fe deficiency or excess dramatically limit plant and algae productivity. Interestingly, complex and unexpected ... [more ▼]

In plants, iron (Fe) transport and homeostasis are highly regulated processes. Fe deficiency or excess dramatically limit plant and algae productivity. Interestingly, complex and unexpected interconnections between Fe and various macro- and micronutrient homeostatic networks, supposedly maintaining general ionic equilibrium and balanced nutrition, are currently uncovered. Although these phenomena have profound consequences on our understanding of Fe homeostasis and its regulation, the molecular bases and biological significance of these interactions remain poorly understood. Here, we review recent knowledge gained on how Fe interacts with micronutrient (e.g. zinc, manganese) and macronutrient (e.g. sulfur, phosphate) homeostasis, and on how these interactions affect Fe uptake and trafficking. We finally highlight the importance of developing an improved model of how Fe signaling pathways are integrated into functional networks to control plant growth and development in response to fluctuating environments. [less ▲]

Detailed reference viewed: 50 (11 ULiège)
Full Text
Peer Reviewed
See detailPhysiology and Molecular Biology of Trace Element Hyperaccumulation
Merlot, Sylvain; Garcia de la Torre, Vanesa Sanchez; Hanikenne, Marc ULiege

in van der Ent, Antony; Baker, Alan; Echevarria, Guillaume (Eds.) et al Agromining: Farming for Metals - Extracting Unconventional Resources Using Plants, 2d edition (2021)

Detailed reference viewed: 52 (9 ULiège)
Full Text
See detailA MAGIC design to study metal homeostasis in Chlamydomonas reinhardtii
Cardoso Esteves, Sara Marina ULiege; Iacono, Fabrizio ULiege; Jadoul, Alice ULiege et al

Poster (2020, November 17)

Natural trait variation is present across all domains of life. Individuals of the same species often present differences in response to environmental stress or adaptation. Some traits, such as biomass or ... [more ▼]

Natural trait variation is present across all domains of life. Individuals of the same species often present differences in response to environmental stress or adaptation. Some traits, such as biomass or High Value Molecule production, have economical interest in the industrial, pharmaceutical or agricultural sectors. Identifying the genetic variants affecting important biological processes is, therefore, fundamental. Chlamydomonas reinhardtii is the reference unicellular photosynthetic eukaryote model organism. C. reinhardtii is a haploid unicellular green alga capable of sexual reproduction, metabolize alternative carbon sources, growing in the dark and under nutrient deficiency. Furthermore, C. reinhardtii has very short generation interval, a high recombination rate and a relatively small genome, making it a suitable organism for a MAGIC design. In model organisms, genetic variation can be studied using Multiparent Advanced Generation Inter-Cross (MAGIC) designs. In these designs, lines of founders presenting phenotypic variation are intercrossed in a determined design where each founder line contributes equally. This process originates terminal lines where the initial variability is shuffled, forming a genetic mosaic of the founder lines. Three MAGIC designs, consisting of 8 initial founders intercrossed for 8 generations (F8) were made, and a total of 768 progenies were selected in order to study mineral nutrition. Metal homeostasis is assured by an intricate network of metal uptake, chelation, trafficking, and storage processes. Mixotrophy was used as control condition, and 10 conditions were tested: autotrophy, macronutrient deprivation (Ca, Mg, N, P, S), and micronutrient deprivation (Cu, Fe, Mn, Zn), in 96h assays. Here we present the preliminary results of a small subset. [less ▲]

Detailed reference viewed: 43 (7 ULiège)
Full Text
Peer Reviewed
See detailDeletion of bglC triggers a genetic compensation response by awakening the expression of alternative beta-glucosidase
Deflandre, Benoit ULiege; Thiébaut, Noémie ULiege; Planckaert, Sören et al

in Biochimica et Biophysica Acta. Gene Regulatory Mechanisms (2020), 1863(10),

In the plant pathogen Streptomyces scabies, the gene bglC encodes a GH1 family cellobiose beta-glucosidase that is both required for primary metabolism and for inducing virulence of the bacterium ... [more ▼]

In the plant pathogen Streptomyces scabies, the gene bglC encodes a GH1 family cellobiose beta-glucosidase that is both required for primary metabolism and for inducing virulence of the bacterium. Deletion of bglC (strain ΔbglC) surprisingly resulted in the augmentation of the global beta-glucosidase activity of S. scabies. This paradoxical phenotype is highly robust as it has been observed in all bglC deletion mutants independently generated, thereby highlighting a phenomenon of genetic compensation. Comparative proteomics allowed to identify two glycosyl hydrolases – named BcpE1 and BcpE2 – of which peptide levels were significantly increased in strain ΔbglC. Quantitative RT-PCR revealed that the higher abundance of BcpE1 and BcpE2 is triggered at the transcriptional level, the expression of their respective gene being 100 and 15 times upregulated. Enzymatic studies with pure BcpE proteins showed that they both possess beta-glucosidase activity thereby explaining the genotypic-phenotypic discrepancy of the bglC deletion mutant. The GH1 family BcpE1 could hydrolyze cellobiose and generate glucose similarly to BglC itself thereby mainly contributing to the survival of strain ΔbglC when cellobiose is provided as sole nutrient source. The low affinity of BcpE2 for cellobiose suggests that this GH3 family beta-glucosidase would instead primarily target another and yet unknown glucose-beta-1,4-linked substrate. These results make S. scabies a new model system to study genetic compensation. Discovering how, either the bglC DNA locus, its mRNA, the BglC protein, or either its enzymatic activity controls bcpE genes' expression, will unveil new mechanisms directing transcriptional repression. [less ▲]

Detailed reference viewed: 70 (17 ULiège)
Full Text
Peer Reviewed
See detailThe two copies of the zinc and cadmium ZIP6 transporter of Arabidopsis halleri have distinct effects on cadmium tolerance.
Spielmann, Julien ULiege; Ahmadi, Hassan; Scheepers, Maxime ULiege et al

in Plant, cell & environment (2020)

Plants have the ability to colonize highly diverse environments. The zinc and cadmium hyperaccumulator Arabidopsis halleri has adapted to establish populations on soils covering an extreme range of metal ... [more ▼]

Plants have the ability to colonize highly diverse environments. The zinc and cadmium hyperaccumulator Arabidopsis halleri has adapted to establish populations on soils covering an extreme range of metal availabilities. The A. halleri ZIP6 gene presents several hallmarks of hyperaccumulation candidate genes: it is constitutively highly expressed in roots and shoots and is associated with a zinc accumulation quantitative trait locus. Here, we show that AhZIP6 is duplicated in the A. halleri genome. The two copies are expressed mainly in the vasculature in both A. halleri and A. thaliana, indicative of conserved cis regulation, and acquired partial organ specialization. Yeast complementation assays determined that AhZIP6 is a zinc and cadmium transporter. AhZIP6 silencing in A. halleri or expression in A. thaliana alters cadmium tolerance, but has no impact on zinc and cadmium accumulation. AhZIP6-silenced plants display reduced cadmium uptake upon short-term exposure, adding AhZIP6 to the limited number of Cd transporters supported by in planta evidence. Altogether, our data suggest that AhZIP6 is key to fine-tune metal homeostasis in specific cell-types. This study additionally highlights the distinct fates of duplicated genes in A. halleri. This article is protected by copyright. All rights reserved. [less ▲]

Detailed reference viewed: 39 (7 ULiège)
Full Text
Peer Reviewed
See detaildi-Cysteine Residues of the Arabidopsis thaliana HMA4 C-Terminus Are Only Partially Required for Cadmium Transport
Ceasar, S. A.; Lekeux, Gilles ULiege; Motte, Patrick ULiege et al

in Frontiers in Plant Science (2020), 11

Cadmium (Cd) is highly toxic to the environment and humans. Plants are capable of absorbing Cd from the soil and of transporting part of this Cd to their shoot tissues. In Arabidopsis, the plasma membrane ... [more ▼]

Cadmium (Cd) is highly toxic to the environment and humans. Plants are capable of absorbing Cd from the soil and of transporting part of this Cd to their shoot tissues. In Arabidopsis, the plasma membrane Heavy Metal ATPase 4 (HMA4) transporter mediates Cd xylem loading for export to shoots, in addition to zinc (Zn). A recent study showed that di-Cys motifs present in the HMA4 C-terminal extension (AtHMA4c) are essential for high-affinity Zn binding and transport in planta. In this study, we have characterized the role of the AtHMA4c di-Cys motifs in Cd transport in planta and in Cd-binding in vitro. In contrast to the case for Zn, the di-Cys motifs seem to be partly dispensable for Cd transport as evidenced by limited variation in Cd accumulation in shoot tissues of hma2hma4 double mutant plants expressing native or di-Cys mutated variants of AtHMA4. Expression analysis of metal homeostasis marker genes, such as AtIRT1, excluded that maintained Cd accumulation in shoot tissues was the result of increased Cd uptake by roots. In vitro Cd-binding assays further revealed that mutating di-Cys motifs in AtHMA4c had a more limited impact on Cd-binding than it has on Zn-binding. The contributions of the AtHMA4 C-terminal domain to metal transport and binding therefore differ for Zn and Cd. Our data suggest that it is possible to identify HMA4 variants that discriminate Zn and Cd for transport. © Copyright © 2020 Ceasar, Lekeux, Motte, Xiao, Galleni and Hanikenne. [less ▲]

Detailed reference viewed: 40 (4 ULiège)
Full Text
Peer Reviewed
See detailIntertwined metal homeostasis, oxidative and biotic stress responses in the Arabidopsis frd3 mutant
Scheepers, Maxime ULiege; Spielmann, Julien ULiege; Boulanger, Madeleine ULiege et al

in Plant Journal (2020), 102

FRD3 (FERRIC REDUCTASE DEFECTIVE 3) plays a major role in iron (Fe) and zinc (Zn) homeostasis in Arabidopsis. It transports citrate, which enables metal distribution in the plant. A frd3 mutant is dwarf ... [more ▼]

FRD3 (FERRIC REDUCTASE DEFECTIVE 3) plays a major role in iron (Fe) and zinc (Zn) homeostasis in Arabidopsis. It transports citrate, which enables metal distribution in the plant. A frd3 mutant is dwarf and chlorotic and displays constitutive Fe deficiency response and strongly altered metal distribution in tissues. Here, we have examined the interaction between Fe and Zn homeostasis in a frd3 mutant exposed to varying Zn supply. Detailed phenotyping using transcriptomic, ionomic, histochemical and spectroscopic approaches revealed the full complexity of the frd3 mutant phenotype, which resulted from altered transition metal homeostasis, manganese toxicity, oxidative and biotic stress responses. The cell wall played a key role in these processes, as a site for Fe and hydrogen peroxide accumulation, and displayed modified structure in the mutant. Finally, we showed that Zn excess interfered with these mechanisms and partially restored root growth of the mutant, without reverting the Fe deficiency response. In conclusion, the frd3 mutant molecular phenotype is more complex than previously described, and illustrates how the response to metal imbalance depends on multiple signaling pathways. [less ▲]

Detailed reference viewed: 76 (17 ULiège)
Full Text
See detailAnalysing the molecular and phenotypic effects of overexpression of JOINTLESS gene in tomato
Huerga Fernandez, Samuel ULiege; Orman-Ligeza, Beata ULiege; Detry, Nathalie ULiege et al

Poster (2019, November 29)

JOINTLESS (J) is a MADS-box gene that regulates inflorescence traits in tomato: its mutation reduces the number of flowers in the inflorescence, which reverts to leaf initiation, and suppresses the ... [more ▼]

JOINTLESS (J) is a MADS-box gene that regulates inflorescence traits in tomato: its mutation reduces the number of flowers in the inflorescence, which reverts to leaf initiation, and suppresses the abscission zone (AZ) in the flower pedicels. It has been shown that the J protein physically interacts with other MADS-box transcription factors, MACROCALYX (MC) and JOINTLESS-2 (J-2) previously known as SlMBP21. Their putative targets are genes involved in AZ initiation since j, j-2 and mc mutants share this common phenotype. For a better understanding of the role of J in the architecture of the inflorescence, overexpression lines have been generated. A RNA-seq approach, comparing overexpression and wild-type lines, has been used to identify potential J-targets and better understand its molecular function. [less ▲]

Detailed reference viewed: 73 (9 ULiège)
Full Text
See detailImpact of the sequencing coverage in metagenetic and metagenomic studies
Bertrand, Amandine ULiege; Detry, Emilie ULiege; Nouet, Cécile ULiege et al

Poster (2019, November 28)

Detailed reference viewed: 48 (13 ULiège)
See detailGenetic Mapping of Quantitative Trait Loci (QTLs) involved in photosynthesis in Chlamydomonas reinhardtii
Iacono, Fabrizio ULiege; Cardoso Esteves, Sara Marina ULiege; Houet, Rebecca ULiege et al

Poster (2019, July 21)

Multi-Parent Advanced Generation Inter-Cross (MAGIC) populations have been developed for several model organisms proving to be a powerful mapping resource in quantitative genetics. Chlamydomonas ... [more ▼]

Multi-Parent Advanced Generation Inter-Cross (MAGIC) populations have been developed for several model organisms proving to be a powerful mapping resource in quantitative genetics. Chlamydomonas reinhardtii is a unicellular haploid green alga studied for decades as model organism to understand the nature and dynamics of photosynthesis. The genome of this species has been previously shown to harbor extensive genetic variation; a precious source of information to dissect the polygenic nature of quantitative traits. Here, we use eight divergent founder strains of C. reinhardtii to develop a MAGIC population constituted of about one thousand Advanced Inter-Crossed Lines (AILs). Once created, the MAGIC population will be employed to map quantitative trait loci (QTLs) correlated to photosynthesis and growth. [less ▲]

Detailed reference viewed: 135 (24 ULiège)
See detailExperimental Evolution of Cadmium Tolerance in Chlamydomonas
Hanikenne, Marc ULiege

Scientific conference (2019, June 28)

Detailed reference viewed: 18 (1 ULiège)
Full Text
See detailWhole Plant Phenotyping and Molecular Identification of Zn Transporters During Zn Deficiency and Excess in Brachypodium distachyon
Amini, Sahand ULiege; Arsova, Borjana; Watt, Michelle et al

Poster (2019, June)

Zinc (Zn) is an essential micronutrient for plants and around two billion people are depending on legumes and grains as their main Zn source. This transition metal is however toxic for plants at high ... [more ▼]

Zinc (Zn) is an essential micronutrient for plants and around two billion people are depending on legumes and grains as their main Zn source. This transition metal is however toxic for plants at high concentrations in soils and affects biomass and yield. The eudicot model plant Arabidopsis thaliana has already been characterized for Zn homeostasis under various Zn regimes and recently we have investigated the transcriptome, proteome and ionome upon Zn deficiency and re-supply in this species (Arsova, B. et al., 2019, bioRxiv, 600569). Although some important players of Zn uptake and transport, such as ZRT, IRT-like Protein (ZIP) family genes, have been studied in wheat, rice and barley, it is unclear how different or similar are the responses of monocots and dicots to Zn supply at the molecular level. In the current study, we aim at characterizing Zn regulation in the model plant Brachypodium distachyon. For this, 10-day-old Brachypodium plants were treated with high- and low-Zn medium for two and three weeks. We measured root length, shoot weight and leaf area to examine how Zn supply influences Brachypodium morphology. Inductively coupled plasma atomic emission spectroscopy (ICP-AES) analysis was performed to determine Zn and other metal ion distribution in root and shoot under different Zn treatments. Quantitative RT-PCR analysis of 20 genes orthologous to Arabidopsis Zn homeostasis genes revealed their expression pattern in response to Zn depletion and surplus. We observed the most commonality between Brachypodium and Arabidopsis, as well as the highest correlation between Zn content and gene regulation, for ZIP genes. In general, our results show that comparative molecular study can be useful to reveal the Zn regulatory mechanisms in monocots, which can lead to new Zn biofortification and stress resistance strategies in vital grains. [less ▲]

Detailed reference viewed: 89 (9 ULiège)