References of "Gustin, Pascal"
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See detailMyopathie atypique : les différentes sources d’intoxication. Comment gérer le risque ?
Renaud, Benoît ULiege; François, Anne-Christine ULiege; Marcillaud-Pitel, C. et al

in Proceedings Journées Sciences et Innovations équines (2019, May)

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See detailMyopathie atypique : les sources connues et suspectées d’intoxication
Renaud, Benoît ULiege; François, Anne-Christine ULiege; Rouxhet, Serge ULiege et al

in Pratique Vétérinaire Équine (2019), 201

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See detailPoints clés de la gestion clinique des cas de myopathie atypique
François, Anne-Christine ULiege; Renaud, Benoît ULiege; Cesarini Latorre, Carlota ULiege et al

in Pratique Vétérinaire Équine (2019), 201

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See detailPotential new sources of hypoglycin A poisoning for equids kept at pasture in spring: a field pilot study
Votion, Dominique ULiege; Habyarimana, J.A.; Scippo, Marie-Louise ULiege et al

in Veterinary Record: Journal of the British Veterinary Association (2019)

The first objective of this pilot study was to measure the concentration of HGA in samaras of sycamore trees fallen on the ground and then in seedlings from germination. The subsequent objective was to ... [more ▼]

The first objective of this pilot study was to measure the concentration of HGA in samaras of sycamore trees fallen on the ground and then in seedlings from germination. The subsequent objective was to examine other possible sources of HGA intoxication. [less ▲]

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See detailIn vitro testing of equine atypical myopathy induced mitochondrial dysfunction
Zhou, Y; Gustin, Pascal ULiege; Mouithys-Mickalad, Ange ULiege et al

Poster (2018, October 26)

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See detailTriamcinolone acetonide after intra-articular injection in knee in sheep
Legrand, Nathalie ULiege; Tian, Jin; Douny, Caroline ULiege et al

Poster (2018, October 26)

Intra-articular injections of glucocorticoids aim to control pain and inflammation caused by osteoarthritis. There is a lack of evidence and pharmacokinetics (PK) studies to support the empirical ... [more ▼]

Intra-articular injections of glucocorticoids aim to control pain and inflammation caused by osteoarthritis. There is a lack of evidence and pharmacokinetics (PK) studies to support the empirical currently used dosage regimens. This study aimed 1) to determine the PK of triamcinolone hexacetonide (TH) and triamcinolone acetonide, its active metabolite (TA), in synovial fluid after intra-articular administration of a suspension of TH at 40mg and 10mg in sheep and 2) to compare the profiles of TA after injection of suspensions of TA or TH, both at 40mg. Twelve sheep were randomly allocated to three groups receiving respectively 40 mg TA (n=4), 40 mg TH (n=4) or 10 mg TH (n=4) in the left knee. Synovial fluids were sampled from day 1 up to day 21. The concentrations of TA and TH were measured by ultra-performance liquid chromatography mass spectrometry. TA concentrations measured after one day were higher in the group TA-40 mg (537762.3ng/ml) compared to those recorded in the group TH-40mg (22743.4ng/ml), On day 21, the corresponding values were 2.5 and 33.5 ng/ml due to a significant higher value of T1/2β of TA in group TH-40 mg (6.0 versus 1.9 days). The differences between the mean values of AUC and T1/2β of TA were not significantly different between the groups TH-10 and -40 mg but T1/2β of TH was significantly higher in the group TH-40mg. In conclusion, TH injection maintains TA concentrations for a longer period of time than TA administration. Due to a possible saturation of esterases, the PK profiles associated to the high and low doses of TH were rather close suggesting that a dose of 10mg could provide an optimal benefit-risk. [less ▲]

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See detailFunctional diagnosis of mitochondrial defects in equine atypical myopathy and targeted mitochondrial therapy: a preliminary study.
Zhou, Y; Gonzalez-Medina; Gustin, Pascal ULiege et al

in In proceedings: 13th Conference on Mitochondrial Physiology (2018, September)

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See detailÉmergence de la toxicité printanière et influence des conditions météorologiques sur les risques de myopathie atypique
Habyarimana, J.A.; Gustin, Pascal ULiege; Scippo, Marie-Louise ULiege et al

in Proceeding de la 44ème Journée de la Recherche équine (2018, March)

Atypical myopathy (AM) equine in Europe results from the ingestion of hypoglycin A (HGA) contained in samara and seedlings of sycamore maple (Acer pseudoplatanus). The concentration of HGA was determined ... [more ▼]

Atypical myopathy (AM) equine in Europe results from the ingestion of hypoglycin A (HGA) contained in samara and seedlings of sycamore maple (Acer pseudoplatanus). The concentration of HGA was determined in samaras fallen on the ground and in seedlings of sycamore maple harvested every 15 days, in spring 2016. Other samples have been also collected: flowers, sycamore saplings and hay. Based on the maximum concentrations of HGA measured in the sycamore samples and extrapolating the toxic dose to a horse from the literature data (dose based on laboratory animals), it can be estimated that at certain times less than 20 g samaras or less than 50 seedlings are enough to reach the maximum tolerated dose for a horse. In addition, this hypothetical toxic dose can be reached with 150 g of inflorescences and 2 L of rainwater that has dripped onto seedlings. The hay produced on one of the grasslands contained HGA in a concentration depending on the number of seedlings dried and included in it. [less ▲]

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See detailDevelopment of an HPTLC method for determination of hypoglycin A in aqueous extracts of seedlings and samaras of Acer species.
Habyarimana, Jean Belt Adélite ULiege; Baise, Etienne; Douny, Caroline ULiege et al

E-print/Working paper (2017)

Hypoglycin A (HGA) is a toxin contained in seeds of the sycamore maple tree (Acer pseudoplatanus). Ingestion of this amino acid causes equine atypical myopathy (AM) in Europe. Another variety, A. negundo ... [more ▼]

Hypoglycin A (HGA) is a toxin contained in seeds of the sycamore maple tree (Acer pseudoplatanus). Ingestion of this amino acid causes equine atypical myopathy (AM) in Europe. Another variety, A. negundo, is claimed to be present where AM cases were reported in the US. For unknown reasons, occurrence of this disease has increased. It is important to define environmental key factors that may influence toxicity of samaras from Acer species. In addition, the content of HGA in seedlings needs to be determined since AM outbreaks, during autumn period when the seeds fall but also during spring when seeds are germinating. The present study aims to validate a reliable method using high performance thin layer chromatography for determination and comparison of HGA in samaras and seedlings. The working range of the method was between 20 μg HGA to 408 μg HGA per ml water, corresponding to 12 - 244 mg/kg fresh weight or 40 - 816 mg/kg dry weight, taking into account of an arbitrary average dry matter content of 30%. Instrumental limit of detection and limit of quantification were of 10 µg HGA/ml and 20 µg HGA/ml water, respectively. Instrumental precision was 4% (RSD on 20 repeated measurements) while instrumental accuracy ranged between 86% and 121% of expected value. The HGA recovery of the analytical method estimated from spiked samaras and seedlings samples ranged between 63 and 103%. The method was applied to 9 samples of samaras from Acer pseudoplatanus, A. platanoides and A. campestre and 5 seedlings samples from A. pseudoplatanus. The results confirm detection of HGA in samaras from A. pseudoplatanus and the absence of detection in samaras of other tested species. They also suggest that detected levels of HGA are highly variable. This confirmed the suitability of the method for HGA detection in samaras or seedling. [less ▲]

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See detailPoint sur les travaux de recherche sur la myopathie atypique et leurs impacts sur la filière: quoi de neuf ?
Marcillaud-Pitel; Baise, Etienne; BOEMER, François ULiege et al

in Proceeding de la 43ème Journée de la Recherche équine (2017)

In 2014, a toxic metabolite, methylenecyclopropyl acetic acid, was found in equine blood suffering from atypical myopathy. This toxic molecule results from the metabolism of hypoglycine A, an amino acid ... [more ▼]

In 2014, a toxic metabolite, methylenecyclopropyl acetic acid, was found in equine blood suffering from atypical myopathy. This toxic molecule results from the metabolism of hypoglycine A, an amino acid contained in the fruits (i.e. samaras) of sycamore maple (Acer pseudoplatanus). From there, the researches performed aimed at (1) identifying the environmental causes responsible for the emergence of the disease; (2) improving case management through improved diagnosis and a better assessment of the vital prognosis, and (3) at better understanding the pathological process in order to identify potentially therapeutic substances. This article describes the research carried out since the discovery of the cause and the practical applications resulting from the results of these researches. [less ▲]

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See detailResidues in beeswax: a health risk for the consumer of honey and beeswax ?
Wilmart, O; Legrève, A; Scippo, Marie-Louise ULiege et al

in Journal of Agricultural and Food Chemistry (2016), 64(44), 84258434

A scenario analysis in regard to the risk of chronic exposure of consumers to residues through the consumption of contaminated honey and beeswax was conducted. Twenty-two plant protection products and ... [more ▼]

A scenario analysis in regard to the risk of chronic exposure of consumers to residues through the consumption of contaminated honey and beeswax was conducted. Twenty-two plant protection products and veterinary substances of which residues have already been detected in beeswax in Europe were selected. The potential chronic exposure was assessed by applying a worst-case scenario based on the addition of a “maximum” daily intake through the consumption of honey and beeswax to the theoretical maximum daily intake through other foodstuffs. For each residue, the total exposure was finally compared to the acceptable daily intake. It is concluded that the food consumption of honey and beeswax contaminated with these residues considered separately does not compromise the consumer’s health, provided proposed action limits are met. In regard to residues of flumethrin in honey and in beeswax, “zero tolerance” should be applied. [less ▲]

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