References of "Gridelet, Virginie"
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See detailComparison between paraffin and mineral oil covering on early human embryo culture: a prospective randomized study.
Labied, Soraya ULiege; Jouan, Caroline ULiege; Wenders, Frederic ULiege et al

in Systems Biology in Reproductive Medicine (2018)

The oil overlay in microdrop culture systems prevents medium evaporation, helps to maintain appropriate pH and osmotic conditions and protects from microbial contamination. In the present study, we ... [more ▼]

The oil overlay in microdrop culture systems prevents medium evaporation, helps to maintain appropriate pH and osmotic conditions and protects from microbial contamination. In the present study, we prospectively compared covering by Ovoil, a paraffin oil, and LiteOil(R), a mineral oil, on the in vitro development of human embryos and their suitability for transfer/freezing at day 3 and live birth rate. One hundred and one patients undergoing in vitro fertilization (IVF) treatment by intracytoplasmic sperm injection (ICSI) were enrolled in our study. After ICSI, 1237 oocytes were 1:1 randomly allocated into 2 groups according to the type of overlaying oil: Ovoil (616 oocytes) or LiteOil(R) (621 oocytes). Fertilization rate was assessed around 18 hours post-insemination (hpi) and embryos were checked for early cleavage at 25 hpi. Embryo morphology was recorded on days 2 and 3. A total of 437 (Ovoil) and 438 day 3 embryos (LiteOil(R)) were analyzed. There were no differences between the two groups in terms of fertilization rate and occurrence of early cleavage. The proportion of top quality embryos (41.7% vs. 41.2%) and the final utilization rates (92.2% vs. 92.0%) were similar in Ovoil and LiteOil groups, respectively, at day 3. Live birth rate per transfer was essentially the same with Ovoil overlay (26.9%) when compared to LiteOil(R) (26.2%). Live birth rate in patients who simultaneously received embryos from both overlay types was 17.2%. Despite the different characteristics of these two oils regarding hydrocarbon saturation, packing and temperature storage, Ovoil and LiteOil(R) can be used in parallel in the same IVF protocol. ABBREVIATIONS: ART: assisted reproductive technologies; hpi: hours post-insemination; hSA: human serum albumin; HTF: human tubal fluid; ICSI: intracytoplasmic sperm injection; IVF: in vitro fertilization; MII: metaphase II; MEA: mouse embryo assay; RT: room temperature. [less ▲]

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See detailImpact of high magnification sperm selection on neonatal outcomes: a retrospective study.
GASPARD, Olivier ULiege; VANDERZWALMEN, Pierre; WIRLEITNER, Barbara et al

in Journal of Assisted Reproduction and Genetics (2018), 35(6), 1113-1121

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See detailAccumulation of IL-17+ Vgamma6+ gamma-delta T cells in pregnant mice is not associated with spontaneous abortion
Polese, Barbara ULiege; Gridelet, Virginie ULiege; Perrier d'Hauterive, Sophie ULiege et al

in Clinical and Translational Immunology (2018), 7

Introduction. Pregnancy is an immune paradox. While the immune system is required for embryo implantation, placental development and progression of gestation, excessive inflammation is associated with ... [more ▼]

Introduction. Pregnancy is an immune paradox. While the immune system is required for embryo implantation, placental development and progression of gestation, excessive inflammation is associated with pregnancy failure. Similarly, the cytokine IL-17A plays an important role in defence against extracellular pathogens, but its dysregulation can lead to pathogenic inflammation and tissue damage. Although expression of IL-17 has been reported during pregnancy, the cellular source of this cytokine and its relevance to gestation are not clear. Objectives. Here we define the kinetics and cellular source of IL-17A in the uterus during healthy and abortion-prone murine pregnancy. Methods. The CBA/J x DBA/2J abortion-prone mating was used and compared to CBA/J x BALB/c control mating. Results. We demonstrate that, irrespective of gestational health, the number of IL-17-producing cells peaks during midterm pregnancy and is largely derived from the gd T-cell lineage. We identify cd T, Th17, CD8 T and NKT cells as the cellular source of IL-17A in pregnant mice. Furthermore, we positively identify the Vc6+ subset of uterine gd T cells as the main producer of IL-17A during both healthy pregnancy and abortive pregnancy. Conclusions. To conclude, the accumulation of uterine IL-17+ innate-like T cells appears not to adversely impact the developing foetus. Collectively, our results show that IL-17+ gd T cells are present in the uterus throughout the course of normal gestation and therefore may play an important role in healthy pregnancy. Keywords: abortion, gamma delta T cells, interleukin-17, pregnancy. [less ▲]

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See detailTreg, Th17 and γδ T cells during normal and abortive pregnancy
Polese, Barbara ULiege; Gridelet, Virginie ULiege; Munaut, Carine ULiege et al

Poster (2016, October 14)

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See detailTreg, Th17 and γδ T cells during normal and abortive pregnancy
Polese, Barbara ULiege; Gridelet, Virginie ULiege; Munaut, Carine ULiege et al

Conference (2016, October 14)

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See detailTreg, Th17 and γδ T cells during normal and abortive pregnancy
Polese, Barbara ULiege; Gridelet, Virginie ULiege; Munaut, Carine ULiege et al

Poster (2016, January 25)

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See detailTreg/Th17 balance in murine embryo implantation and pregnancy
Polese, Barbara ULiege; Gridelet, Virginie ULiege; Araklioti, Eleni ULiege et al

Poster (2015, March)

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See detailNeonatal outcomes after IMSI and ICSI at the CPMA of the University of Liège.
GASPARD, Olivier ULiege; Danthine, Denis; RAVET, Stéphanie ULiege et al

Poster (2015)

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See detailTreg/Th17 balance during murine embryo implantation and pregnancy
Polese, Barbara ULiege; Gridelet, Virginie ULiege; Araklioti, Eleni et al

Poster (2014, November)

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See detailThe endocrine milieu and CD4 T-lymphocyte polarization during pregnancy
Polese, Barbara ULiege; Gridelet, Virginie ULiege; Arakioti, Eleni et al

in Frontiers in Endocrinology (2014), 5(Article 106), 1-11

Acceptance of the fetal semi-allograft by the mother’s immune system has become the focus of intensive research. CD4+ T cells are important actors in the establishment of pregnancy. Th1/Th2 paradigm has ... [more ▼]

Acceptance of the fetal semi-allograft by the mother’s immune system has become the focus of intensive research. CD4+ T cells are important actors in the establishment of pregnancy. Th1/Th2 paradigm has been expanded to include CD4+ regulatory T (Treg) and T helper 17 (Th17) cells. Pregnancy hormones exert very significant modulatory properties on the maternal immune system. In this review, we describe mechanisms by which the endocrine milieu modulates CD4 T cell polarization during pregnancy. We first focused on Treg and Th17 cells and on their importance for pregnancy. Secondly, we review the effects of pregnancy hormones [progesterone (P4) and estradiol (E2)] on immune cells previously described, with a particular attention to human chorionic gonadotropin (hCG). The importance of Treg cells for pregnancy is evidenced. They are recruited before implantation and are essential for pregnancy maintenance. Decreased number or less efficient Treg cells are implicated in fertility disorders. As for Th17 cells, the few available studies suggest that they have a negative impact on fertility. Th17 frequency is increased in infertile patients. With the combination of its pro-effects on Th2 and Treg cells and anti-effects on Th1 and Th17 cells, P4 contributes to establishment of a favorable environment for pregnancy. E2 effects are more dependent on the context but it seems that E2 promotes Treg and Th2 cells while it inhibits Th1 cells. hCG positively influences activities of Treg and uterine natural killer cells. This embryo signal is an essential actor for the success of pregnancy, both as the endocrine factor regulating P4 secretion by the ovarian corpus luteum, but also as a paracrine agent during implantation as well as an angiogenic and immunologic mediator during the course of gestation. Luteinizing hormone (LH) immune properties begin to be studied but its positive impact on Treg cells suggests that LH could be a considerable immunomodulator in the mouse. [less ▲]

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See detailFive years of IMSI at CPMA-University of Liège
GASPARD, Olivier ULiege; RAVET, Stéphanie ULiege; WENDERS, Frédéric ULiege et al

Poster (2014)

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See detailEvidence for cross-talk between the LH receptor and LH during implantation in mice
Gridelet, Virginie ULiege; Tsampalas, Marie; Berndt, Sarah et al

in Reproduction, Fertility and Development (2013), 25

The present study investigated the first interaction that occurs between the blastocyst and endometrium during implantation. Given the ethical objections to studying implantation in humans, a mouse model ... [more ▼]

The present study investigated the first interaction that occurs between the blastocyst and endometrium during implantation. Given the ethical objections to studying implantation in humans, a mouse model was used to study the dialogue between luteinising hormone (LH) and luteinising hormone receptor (LHCGR). Several studies performed on LHCGR-knockout mice have generated controversy regarding the importance of the dialogue between LH and LHCGR during implantation. There has been no demonstration of a bioactive LH-like signal produced by the murine blastocyst. The first aim of the present study was to examine and quantify, using radioimmunoassay, the generation of a bioactive LH signal by the murine blastocyst. We went on to examine and quantify endometrial Lhcgr expression to validate the mouse model. Expression of LHCGR in mouse uteri was demonstrated using immunohistochemistry and western blot analysis. To quantify the expression of Lh in the mouse blastocyst and Lhcgr in the endometrium, reverse transcription–polymerase chain reaction (RT-PCR) and real-time quantitative (q) RT-PCR were performed. The results demonstrate that Lhcgr expression in BALB/c mouse endometrial epithelium is increased at the time of implantation and indicate that LHCGR may contribute to the implantation process. In support of this hypothesis, we identified a bioactive LH signal at the time of murine blastocyst implantation. [less ▲]

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See detailImpact of follicular G-CSF quantification on subsequent embryo transfer decisions: a proof of concept study.
Ledee, N.; Gridelet, Virginie ULiege; RAVET, Stéphanie ULiege et al

in Human Reproduction (2013), 28(2), 406-13

BACKGROUND: Previous experiments have shown that granulocyte colony-stimulating factor (G-CSF), quantified in the follicular fluid (FF) of individual oocytes, correlates with the potential for an ongoing ... [more ▼]

BACKGROUND: Previous experiments have shown that granulocyte colony-stimulating factor (G-CSF), quantified in the follicular fluid (FF) of individual oocytes, correlates with the potential for an ongoing pregnancy of the corresponding fertilized oocytes among selected transferred embryos. Here we present a proof of concept study aimed at evaluating the impact of including FF G-CSF quantification in the embryo transfer decisions. METHODS: FF G-CSF was quantified with the Luminex XMap technology in 523 individual FF samples corresponding to 116 fresh transferred embryos, 275 frozen embryos and 131 destroyed embryos from 78 patients undergoing ICSI. RESULTS: Follicular G-CSF was highly predictive of subsequent implantation. The receiving operator characteristics curve methodology showed its higher discriminatory power to predict ongoing pregnancy in multivariate logistic regression analysis for FF G-CSF compared with embryo morphology [0.77 (0.69-0.83), P < 0.001 versus 0.66 (0.58-0.73), P = 0.01)]. Embryos were classified by their FF G-CSF concentration: Class I over 30 pg/ml (a highest positive predictive value for implantation), Class II from 30 to 18.4 pg/ml and Class III <18.4 pg/ml (a highest negative predictive value). Embryos derived from Class I follicles had a significantly higher implantation rate (IR) than those from Class II and III follicles (36 versus 16.6 and 6%, P < 0.001). Embryos derived from Class I follicles with an optimal morphology reached an IR of 54%. Frozen-thawed embryos transfer derived from Class I follicles had an IR of 37% significantly higher than those from Class II and III follicles, respectively, of 8 and 5% (P < 0.001). Thirty-five per cent of the frozen embryos but also 10% of the destroyed embryos were derived from G-CSF Class I follicles. Non-optimal embryos appear to have been transferred in 28% (22/78) of the women, and their pregnancy rate was significantly lower than that of women who received at least one optimal embryo (18 versus 36%, P = 0.04). CONCLUSIONS: Monitoring FF G-CSF for the selection of embryos with a better potential for pregnancy might improve the effectiveness of IVF by reducing the time and cost required for obtaining a pregnancy. [less ▲]

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See detailThe actors of human implantation: gametes, embryo and endometrium
Gridelet, Virginie ULiege; GASPARD, Olivier ULiege; Polese, Barbara ULiege et al

in Violin Pereira, Luis Antonio (Ed.) Embryology - Updates and Highlights on Classic Topics (2012)

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See detailThe endometrial hCG/LH receptor: quantification throughout the menstrual cycle
Tsampalas, M; Berndt, S; Gridelet, Virginie ULiege et al

Conference (2010, March 04)

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See detailFollicular fluids, immunological fingerprint and infertility
Lédée, N; Munaut, Carine ULiege; PERRIER d'HAUTERIVE, Sophie ULiege et al

Conference (2010)

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