References of "GASPARD, Olivier"
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See detailComparison between paraffin and mineral oil covering on early human embryo culture: a prospective randomized study.
Labied, Soraya ULiege; Jouan, Caroline ULiege; Wenders, Frederic ULiege et al

in Systems Biology in Reproductive Medicine (2018)

The oil overlay in microdrop culture systems prevents medium evaporation, helps to maintain appropriate pH and osmotic conditions and protects from microbial contamination. In the present study, we ... [more ▼]

The oil overlay in microdrop culture systems prevents medium evaporation, helps to maintain appropriate pH and osmotic conditions and protects from microbial contamination. In the present study, we prospectively compared covering by Ovoil, a paraffin oil, and LiteOil(R), a mineral oil, on the in vitro development of human embryos and their suitability for transfer/freezing at day 3 and live birth rate. One hundred and one patients undergoing in vitro fertilization (IVF) treatment by intracytoplasmic sperm injection (ICSI) were enrolled in our study. After ICSI, 1237 oocytes were 1:1 randomly allocated into 2 groups according to the type of overlaying oil: Ovoil (616 oocytes) or LiteOil(R) (621 oocytes). Fertilization rate was assessed around 18 hours post-insemination (hpi) and embryos were checked for early cleavage at 25 hpi. Embryo morphology was recorded on days 2 and 3. A total of 437 (Ovoil) and 438 day 3 embryos (LiteOil(R)) were analyzed. There were no differences between the two groups in terms of fertilization rate and occurrence of early cleavage. The proportion of top quality embryos (41.7% vs. 41.2%) and the final utilization rates (92.2% vs. 92.0%) were similar in Ovoil and LiteOil groups, respectively, at day 3. Live birth rate per transfer was essentially the same with Ovoil overlay (26.9%) when compared to LiteOil(R) (26.2%). Live birth rate in patients who simultaneously received embryos from both overlay types was 17.2%. Despite the different characteristics of these two oils regarding hydrocarbon saturation, packing and temperature storage, Ovoil and LiteOil(R) can be used in parallel in the same IVF protocol. ABBREVIATIONS: ART: assisted reproductive technologies; hpi: hours post-insemination; hSA: human serum albumin; HTF: human tubal fluid; ICSI: intracytoplasmic sperm injection; IVF: in vitro fertilization; MII: metaphase II; MEA: mouse embryo assay; RT: room temperature. [less ▲]

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See detailImpact of high magnification sperm selection on neonatal outcomes: a retrospective study.
GASPARD, Olivier ULiege; VANDERZWALMEN, Pierre; WIRLEITNER, Barbara et al

in Journal of Assisted Reproduction and Genetics (2018), 35(6), 1113-1121

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See detailNeonatal outcomes after IMSI and ICSI at the CPMA of the University of Liège.
GASPARD, Olivier ULiege; Danthine, Denis; RAVET, Stéphanie ULiege et al

Poster (2015)

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See detailSperm Vacuoles : Origin and Implications
Vanderzwalmen, Pierre; Zech, Nicolas; Lejeune, Bernard et al

in Non-Invasive Sperm Selection for in Vitro Fertilization: Novel Concepts and Methods (2015)

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See detailGlucose use and lactate production by equine fresh semen in human and equine extender
Ponthier, Jérôme ULiege; De Tullio, Pascal ULiege; Parrilla-Hernandez, Sonia et al

in Reproduction in Domestic Animals (2014, September), 49(suppl 3), 13

This study shows that this human semen extender doesn’t support equine semen preservation. Sperm cells’ glucose consumption and lactate production seem to be negligible, as these parameters were not ... [more ▼]

This study shows that this human semen extender doesn’t support equine semen preservation. Sperm cells’ glucose consumption and lactate production seem to be negligible, as these parameters were not affected by sperm concentrations in our study. Our results suggest that spermatozoa are able to cleave complex carbohydrates as glucose concentration in INRA96 increased over time. [less ▲]

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See detailMorphological Selection of Gametes and Embryos: Sperm.
Vanderzwalmen, Pierre; Bach, Magnus; GASPARD, Olivier ULiege et al

in A Practical Guide to Selecting Gametes and Embryos (2014)

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See detailFive years of IMSI at CPMA-University of Liège
GASPARD, Olivier ULiege; RAVET, Stéphanie ULiege; WENDERS, Frédéric ULiege et al

Poster (2014)

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See detailImpact of follicular G-CSF quantification on subsequent embryo transfer decisions: a proof of concept study.
Ledee, N.; Gridelet, Virginie ULiege; RAVET, Stéphanie ULiege et al

in Human Reproduction (2013), 28(2), 406-13

BACKGROUND: Previous experiments have shown that granulocyte colony-stimulating factor (G-CSF), quantified in the follicular fluid (FF) of individual oocytes, correlates with the potential for an ongoing ... [more ▼]

BACKGROUND: Previous experiments have shown that granulocyte colony-stimulating factor (G-CSF), quantified in the follicular fluid (FF) of individual oocytes, correlates with the potential for an ongoing pregnancy of the corresponding fertilized oocytes among selected transferred embryos. Here we present a proof of concept study aimed at evaluating the impact of including FF G-CSF quantification in the embryo transfer decisions. METHODS: FF G-CSF was quantified with the Luminex XMap technology in 523 individual FF samples corresponding to 116 fresh transferred embryos, 275 frozen embryos and 131 destroyed embryos from 78 patients undergoing ICSI. RESULTS: Follicular G-CSF was highly predictive of subsequent implantation. The receiving operator characteristics curve methodology showed its higher discriminatory power to predict ongoing pregnancy in multivariate logistic regression analysis for FF G-CSF compared with embryo morphology [0.77 (0.69-0.83), P < 0.001 versus 0.66 (0.58-0.73), P = 0.01)]. Embryos were classified by their FF G-CSF concentration: Class I over 30 pg/ml (a highest positive predictive value for implantation), Class II from 30 to 18.4 pg/ml and Class III <18.4 pg/ml (a highest negative predictive value). Embryos derived from Class I follicles had a significantly higher implantation rate (IR) than those from Class II and III follicles (36 versus 16.6 and 6%, P < 0.001). Embryos derived from Class I follicles with an optimal morphology reached an IR of 54%. Frozen-thawed embryos transfer derived from Class I follicles had an IR of 37% significantly higher than those from Class II and III follicles, respectively, of 8 and 5% (P < 0.001). Thirty-five per cent of the frozen embryos but also 10% of the destroyed embryos were derived from G-CSF Class I follicles. Non-optimal embryos appear to have been transferred in 28% (22/78) of the women, and their pregnancy rate was significantly lower than that of women who received at least one optimal embryo (18 versus 36%, P = 0.04). CONCLUSIONS: Monitoring FF G-CSF for the selection of embryos with a better potential for pregnancy might improve the effectiveness of IVF by reducing the time and cost required for obtaining a pregnancy. [less ▲]

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See detailThe actors of human implantation: gametes, embryo and endometrium
Gridelet, Virginie ULiege; GASPARD, Olivier ULiege; Polese, Barbara ULiege et al

in Violin Pereira, Luis Antonio (Ed.) Embryology - Updates and Highlights on Classic Topics (2012)

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See detailImpact of laser-assisted hatching on pregnancy rate in fresh and frozen-thawed cycles
Thonon, Fabienne ULiege; Gaspard, Olivier ULiege; Jouan, Caroline ULiege et al

in Human Reproduction (2006, June), 21(Suppl. 1), 124

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See detailTwin pregnancy obtained with frozen-thawed embryos after in vitro maturation in a patient with polycystic ovarian syndrome
Godin, P. A.; Gaspard, Olivier ULiege; Thonon, Fabienne ULiege et al

in Journal of Assisted Reproduction and Genetics (2003), 20(8), 347-350

Purpose: A twin pregnancy was obtained in a patient with polycystic ovary syndrome after the transfer of three in vitro maturation-derived day 3 embryos that has been frozen and thawed. Methods: The ... [more ▼]

Purpose: A twin pregnancy was obtained in a patient with polycystic ovary syndrome after the transfer of three in vitro maturation-derived day 3 embryos that has been frozen and thawed. Methods: The patient had received mild hMG stimulation followed by hCG injection. After culture for 24 - 48 h, mature oocytes were fertilized by ICSI. Embryos were cultured until day 3; supernumerary embryos were cryopreserved using a slow protocol. Results: Among 15 nonatretic oocytes, 9 matured, 8 were fertilized. Four embryos were transferred but they did not implant. The subsequent transfer of three frozen - thawed embryos resulted in the delivery of two healthy girls. Conclusions: These results indicate that a pregnancy could be obtained with in vitro maturation-derived day-3 frozen - thawed embryos. [less ▲]

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