References of "Franchina, Flavio"
     in
Bookmark and Share    
See detailMedical Application: Breath Volatile Compounds in Asthma Patients
Stefanuto, Pierre-Hugues ULiege; Zanella, Delphine ULiege; dejong, Thibaut et al

Scientific conference (2019, June)

Detailed reference viewed: 35 (5 ULiège)
Full Text
See detailRapid screening of complex biological matrices: Utilizing kendrick mass defect for group type evaluation of GC×GC-HR(EI)ToF/MS data
Weggler, Benedikt ULiege; Gruber, Beate; Zanella, Delphine ULiege et al

Poster (2019, May 13)

Characterization of highly complex matrices implicates scientific challenges such as wide presence of “true” unknowns, concentration ranges of various compound classes and limited, available amounts of ... [more ▼]

Characterization of highly complex matrices implicates scientific challenges such as wide presence of “true” unknowns, concentration ranges of various compound classes and limited, available amounts of sample. Cutting-edge, discovery based separation techniques such as multidimensional gas chromatography coupled to high-resolution time-of-flight mass spectrometry (GCxGC-HRToF/MS), are commonly applied to such analytical challenges. Nevertheless, most studies focus on target analysis and tend to disregard important details of the sample composition. The high separation capacity of GCxGC-ToF/MS allows for in-depth chemical analysis of the molecular composition. However, high amounts of data, containing several thousands of compounds per experiment, are generally acquired during such analyzes. Coupling GCxGC to high-resolution mass spectrometry (HRMS) further increases the amount of data and therefore requires advanced data reduction and mining techniques. Commonly, the main approach for the evaluation of dense data sets either focuses on the chromatographic separation for e.g. group type analysis, or utilizes exact mass data applying Kendrick Mass Defect (KMD) analysis or van Krevelen plots. The presented approach integrated the accurate mass data into the chromatographic information by combining KMD information and knowledge-based rules. This combination allows for fast, visual data screening as well as first quantitative estimation of the sample's composition. Moreover, the resulted sample classification significantly reduces the number of variables, allowing distinct chemometric analysis in non-targeted studies such as detailed hydrocarbon analysis (DHA), environmental and forensic investigations. [less ▲]

Detailed reference viewed: 27 (6 ULiège)
Full Text
Peer Reviewed
See detailOptimizing of TD-GC×GC-HRTOFMS for the profiling of cannabis blends
Dubois, Lena ULiege; Ledent, Maxime ULiege; Franchina, Flavio ULiege et al

Poster (2019, May 13)

Detailed reference viewed: 27 (3 ULiège)
Full Text
Peer Reviewed
See detailBidimensional gas-chromatography (GCxGC-ToF-MS) to evaluate the volatile profile of grappa
Betnega, P; Longo, E; de Matos, A et al

Poster (2019, May)

Detailed reference viewed: 14 (0 ULiège)
Full Text
Peer Reviewed
See detailImportance du type d'absorbant pour l'échantillonnage des volatils : application sur les cultures bactériennes et l'haleine
Zanella, Delphine ULiege; Franchina, Flavio ULiege; Stefanuto, Pierre-Hugues ULiege et al

Poster (2019, March)

Les composés organiques volatils bactériens (VOC) sont considérés comme des biomarqueurs sensibles et spécifiques pour le phénotypage bactérien dans les biofluides humains (haleine, sang, urine, etc.) et ... [more ▼]

Les composés organiques volatils bactériens (VOC) sont considérés comme des biomarqueurs sensibles et spécifiques pour le phénotypage bactérien dans les biofluides humains (haleine, sang, urine, etc.) et dans les milieux de culture. La possibilité d'utiliser les VOCs pour l'identification bactérienne ouvre de nouvelles possibilités pour la mise au point de techniques de diagnostiques plus efficaces. Outre les différences biologiques des environnements in-vivo et in-vitro, il est essentiel d'utiliser la même technique d’échantillonnage pour la caractérisation et la validation de biomarqueurs. Dans cette étude, la chromatographie gazeuse bidimensionnelle couplée à la spectrométrie de masse (GC×GC-MS) a été utilisée pour comparer et évaluer différents adsorbants de tubes de désorption thermique pour l’échantillonnage des VOCs. Plus précisément, les paramètres suivants ont été évalués pour chaque adsorbant: sensibilité, sélectivité, reproductibilité et linéarité. Cinq adsorbants différents (Carbopack Y, X, B, Carboxen 1000 et Tenax), utilisés individuellement ou en combinaison, ont été testés sur un mélange de standards (15 composés). Les meilleures sensibilité et reproductibilité ont été obtenues pour les tubes conditionnés avec du Tenax. Les deux tubes de désorption thermique les plus performants, Tenax et Carbopack Y + X + Carboxen 1000, ont également été évalués sur des cultures de E. coli, S. aureus et P. aeruginosa. Ces deux types de tubes ont pu distinguer les 3 types de culture bactérienne, mais une amélioration de la sensibilité et de la reproductibilité a été obtenue avec les tubes Tenax. Une comparaison similaire sur les performances des tubes a été effectuée sur des échantillons d'haleine. [less ▲]

Detailed reference viewed: 37 (13 ULiège)
Full Text
Peer Reviewed
See detailEvaluation of different adsorbent materials for the untargeted and targeted bacterial VOC analysis using GC×GC-MS
Franchina, Flavio ULiege; Purcaro, Giorgia ULiege; Burklund, A. et al

in Analytica Chimica Acta (2019), 1066

The analysis of bacterial volatile organic compounds has gained attraction as a non-invasive way to identify disease-causing organisms, given that bacteria have unique metabolisms and volatile metabolic ... [more ▼]

The analysis of bacterial volatile organic compounds has gained attraction as a non-invasive way to identify disease-causing organisms, given that bacteria have unique metabolisms and volatile metabolic byproducts. In the present research, different adsorbent materials (Carbopack Y, X, B, Carboxen 1000 and Tenax TA), packed singularly or in combination, were compared in terms of sampling performance (sensitivity, repeatability and selectivity) for the extraction of standards and bacterial volatile metabolites in vitro (from Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli). After extraction, bacterial volatile organic compounds were desorbed and analyzed in a comprehensive two-dimensional gas chromatography system coupled to a time-of-flight mass spectrometer (GC GC-ToF MS). The results show that Tenax has the greater ability to extract the standard mix as well as volatile organic compounds with better repeatability (4e26 RSD%), higher sensitivity (on average ~24 fold) compared to Carbopack Y, X and Carboxen 1000 tube, which followed in terms of performance. In addition, Tenax confirmed the best sensitivity and discriminatory power with no misclassification in the untargeted and unsupervised analysis for the differentiation of the bacterial species. [less ▲]

Detailed reference viewed: 17 (3 ULiège)
Full Text
Peer Reviewed
See detailBreath metabolome of mice infected with Pseudomonas aeruginosa
Purcaro, Giorgia ULiege; Nasir, M.; Franchina, Flavio ULiege et al

in Metabolomics (2019), 15(1),

Introduction: The measurement of specific volatile organic compounds in breath has been proposed as a potential diagnostic for a variety of diseases. The most well-studied bacterial lung infection in the ... [more ▼]

Introduction: The measurement of specific volatile organic compounds in breath has been proposed as a potential diagnostic for a variety of diseases. The most well-studied bacterial lung infection in the breath field is that caused by Pseudomonas aeruginosa. Objectives: To determine a discriminatory core of molecules in the “breath-print” of mice during a lung infection with four strains of P. aeruginosa (PAO1, PA14, PAK, PA7). Furthermore, we attempted to extrapolate a strain-specific “breath-print” signature to investigate the possibility of recapitulating the genetic phylogenetic groups (Stewart et al. Pathog Dis 71(1), 20–25, 2014. https://doi.org/10.1111/2049-632X.12107). Methods: Breath was collected into a Tedlar bag and shortly after drawn into a thermal desorption tube. The latter was then analyzed into a comprehensive multidimensional gas chromatography coupled with a time-of-flight mass spectrometer. Random forest algorithm was used for selecting the most discriminatory features and creating a prediction model. Results: Three hundred and one molecules were significantly different between animals infected with P. aeruginosa, and those given a sham infection (PBS) or inoculated with UV-killed P. aeruginosa. Of those, nine metabolites could be used to discriminate between the three groups with an accuracy of 81%. Hierarchical clustering showed that the signature from breath was due to a specific response to live bacteria instead of a generic infection response. Furthermore, we identified ten additional volatile metabolites that could differentiate mice infected with different strains of P. aeruginosa. A phylogram generated from the ten metabolites showed that PAO1 and PA7 were the most distinct group, while PAK and PA14 were interspersed between the former two groups. Conclusions: To the best of our knowledge, this is the first study to report on a ‘core’ murine breath print, as well as, strain level differences between the compounds in breath. We provide identifications (by running commercially available analytical standards) to five breath compounds that are predictive of P. aeruginosa infection. © 2019, Springer Science+Business Media, LLC, part of Springer Nature. [less ▲]

Detailed reference viewed: 20 (1 ULiège)
Full Text
Peer Reviewed
See detailIdentification of Mycobacterium tuberculosis using volatile biomarkers in culture and exhaled breath
Mellors, T. R.; Nasir, M.; Franchina, Flavio ULiege et al

in Journal of Breath Research (2019), 13(1),

Detailed reference viewed: 21 (1 ULiège)
Full Text
Peer Reviewed
See detailStudy of the lipid profile of ATCC and clinical strains of staphylococcus aureus in relation to their antibiotic resistance
Bisignano, C.; Ginestra, G.; Smeriglio, A. et al

in Molecules (2019), 24(7),

A number of reports have indicated a relationship between bacterial resistance to antibiotics and their lipid composition. In the present study, we characterized the lipid profiles of American Type ... [more ▼]

A number of reports have indicated a relationship between bacterial resistance to antibiotics and their lipid composition. In the present study, we characterized the lipid profiles of American Type Culture Collection (ATCC) and clinical strains of Staphylococcus aureus and its correlation with antibiotic resistance and hydrophobicity. The following strains were used: S. aureus ATCC 6538P, S. aureus ATCC 43300 (MRSA), seven clinical strains from the pharynges, two strains from duodenal ulcers, four strains from hip prostheses, and one strain from the conjunctiva. Lipid-related differentiation was observed across the S. aureus strains: the higher abundance of anteiso-pentadecanoic acid (anteiso-C 15:0 ) and anteiso-heptadecanoic acid (anteiso-C 17:0 ), followed by iso-pentadecanoic acid (iso-C 15:0 ), suggested that these were common lipids. Iso-tridecanoic acid (iso-C 13:0 ) and anteiso-tridecanoic acid (anteiso-C 13:0 ), iso-hexadecanoic acid (iso-C 16:0 ) and anteiso-hexadecanoic acid (anteiso-C 16:0 ), and all forms of octadecanoic acid (C 18:0 ) were usually detected in low abundance. Strains isolated from pharynges showed the highest ratio of branched/straight chains. A distinction in two clusters based on the amount and type of bacterial lipids identified was obtained, which correlated to the antibiotic resistance, the strains origin, and the cell-surface hydrophobicity. We report a potential correlation between the lipid profile of S. aureus strains, site of infection, antibiotic resistance, and cell-surface hydrophobicity. These results, which still need further insights, could be a first step to identifying antibiotic resistance in response to environmental adaptation. © 2019 by the authors. [less ▲]

Detailed reference viewed: 14 (1 ULiège)
Full Text
Peer Reviewed
See detailPrintable QR code paper microfluidic colorimetric assay for screening volatile biomarkers
Burklund, A.; Saturley-Hall, H. K.; Franchina, Flavio ULiege et al

in Biosensors and Bioelectronics (2019), 128

We present a QR code paper microfluidic colorimetric assay that can exploit the hardware and software on mobile devices, and circumvent sample preparation by directly targeting volatile biomarkers. Our ... [more ▼]

We present a QR code paper microfluidic colorimetric assay that can exploit the hardware and software on mobile devices, and circumvent sample preparation by directly targeting volatile biomarkers. Our platform is a printable microarray of well-defined reaction regions, which outputs an instant diagnosis by directing the user to a URL containing their test result, while simultaneously storing epidemiological data for remote access and bioinformatics. To assist in the rapid identification of Escherichia coli in bloodstream infections, we employed an existing colorimetric reagent (p-dimethylaminocinnamaldehyde) and adapted its use to detect volatile indole, a biomarker produced by E. coli. Our assay was able to quantitatively detect indole in the headspace of E. coli culture after 12 h of growth (27.0 ± 3.1 ppm), assisting in species-level identification hours earlier than existing methods. Results were confirmed with headspace solid-phase microextraction (HS-SPME) two-dimensional gas chromatography time-of-flight mass spectrometry (GC×GC-ToFMS), which estimated indole concentration in E. coli culture to average 32.3 ± 5.2 ppm after 12 h of growth. This QR paper microfluidic platform represents a novel development in both telemedicine and diagnostics using volatile biomarkers. We envision that our QR code platform can be extended to other colorimetric assays for real-time diagnostics in low-resource environments. © 2018 Elsevier B.V. [less ▲]

Detailed reference viewed: 40 (2 ULiège)
Full Text
See detailImportance of sorbent material selection for VOCs sampling: application on bacterial cultures and breath
Franchina, Flavio ULiege; Zanella, Delphine ULiege; Stefanuto, Pierre-Hugues ULiege et al

Scientific conference (2018, November 08)

Bacterial volatile organic compounds (VOCs) have been considered as sensitive and specific biomarkers for bacterial phenotyping in both human biofluids (breath, blood, urine, etc.) and culture media. The ... [more ▼]

Bacterial volatile organic compounds (VOCs) have been considered as sensitive and specific biomarkers for bacterial phenotyping in both human biofluids (breath, blood, urine, etc.) and culture media. The possibility of using VOCs markers for bacterial identification would open a new frontier for developing more efficient diagnostic techniques of infections. Besides the biological differences in in vivo/in vitro environments, the importance of using the same sampling technique and sorbent phase is crucial for the translation and validation of biomarker discovery. In the present contribution, GC×GC-MS was exploited to compare and evaluate different adsorption materials for thermal desorption tubes for VOCs sampling. Specifically, the following parameters were evaluated: sensitivity, selectivity, reproducibility and linear range. Five different adsorbent materials (Carbopack Y, X, B, Carboxen 1000 and Tenax), packed singularly or in combination, were tested on a standard mixture (15 compounds). The tubes packed with Tenax showed the best reproducibility (max 14% RSD) and sensitivity, with ~24 average fold increase compared to Carbopack Y+X+Carboxen 1000, which was second in terms of sensitivity. The two better performing thermal desorption tubes, Tenax and Carbopack Y+X+Carboxen 1000, was also evaluated on E. coli, S. aureus, and P. aeruginosa cultures. Both tubes were able to discriminate between the 3 culture types, but improved sensitivity and reproducibility were obtained with Tenax tubes. A similar comparison on tube performances was carried out on breath samples. [less ▲]

Detailed reference viewed: 52 (16 ULiège)
Full Text
Peer Reviewed
See detailA breathprint of influenza a virus infection in the ferret model
Kormuth, K; Franchina, Flavio ULiege; Le Sage, V et al

Poster (2018, November 08)

Influenza viruses (IV) pose a major public health concern, since they are highly contagious and still have a high global annual mortality. Current diagnostic methods are slow or have high error rates ... [more ▼]

Influenza viruses (IV) pose a major public health concern, since they are highly contagious and still have a high global annual mortality. Current diagnostic methods are slow or have high error rates; therefore, a rapid detection tool is highly desirable to inform clinical management. Exhaled breath is a useful diagnostic in a number of diseases. In this work, we examined the exhaled breath of six ferrets collected pre- and post-IV infection. Exhaled breath analysis was carried out using a comprehensive two-dimensional gas chromatography (GC×GC) hyphenated with a time-of-flight mass spectrometer (ToF MS). Random Forest, a non-parametric machine learning algorithm, was used to select the most relevant diagnostic volatile organic compound (VOC) features and build the model using a cross-validation approach, obtaining an accuracy of 0.973 (out of 1.000). Using this approach, we defined 31 VOC features, which together produce a profile capable of discriminating between uninfected and IV-infected ferrets. Further characterization revealed an abundance of hydrocarbons, which is consistent with increased oxidative stress known to occur during viral infection. Our study is the first of its kind to define a unique exhaled breath signature for influenza infection in ferrets and will be useful for development of a rapid precision diagnostic technique. [less ▲]

Detailed reference viewed: 24 (1 ULiège)
Full Text
Peer Reviewed
See detailEnvironmental conditions influence the biochemical properties of the fruiting bodies of Tuber magnatum Pico
Vita, F.; Franchina, Flavio ULiege; Taiti, C. et al

in Scientific Reports (2018), 8(1),

The influences of various factors, including the symbiosis established with the roots of specific tree species, on the production of volatiles in the fruiting bodies of Tuber magnatum have not been ... [more ▼]

The influences of various factors, including the symbiosis established with the roots of specific tree species, on the production of volatiles in the fruiting bodies of Tuber magnatum have not been investigated yet. Volatiles in T. magnatum fruiting bodies were quantitatively and qualitatively determined by both PTR-MS and GC-MS in order to compare the accuracy of the two methods. An electronic nose was also used to characterize truffle samples. The influence of environmental changes on the antioxidant capabilities of fruiting bodies was also determined. Statistically significant differences were found between fruiting bodies with different origins. The relationship between the quality of white truffle fruiting bodies and their specific host plant is described along with an analysis of metabolites other than VOCs that have ecological roles. Our results indicate that the geographical origin (Italy and Istria) of the fruiting bodies is correlated with the quantity and quality of volatiles and various antioxidant metabolites. This is the first report characterizing antioxidant compounds other than VOCs in white truffles. The correlation between geographical origin and antioxidant contents suggests that these compounds may be useful for certifying the geographical origin of truffles. © 2018 The Author(s). [less ▲]

Detailed reference viewed: 11 (0 ULiège)
Full Text
Peer Reviewed
See detailInvestigation of mycobacteria fatty acid profile using different ionization energies in GC–MS
Beccaria, M.; Franchina, Flavio ULiege; Nasir, M. et al

in Analytical and Bioanalytical Chemistry (2018), 410(30), 7987-7996

Gas chromatography (GC) coupled with electron ionization (EI) mass spectrometry (MS) is a well-established technique for the analysis of volatile and semi-volatile compounds. The main advantage is the ... [more ▼]

Gas chromatography (GC) coupled with electron ionization (EI) mass spectrometry (MS) is a well-established technique for the analysis of volatile and semi-volatile compounds. The main advantage is the highly repeatable fragmentation of the compounds into the ion source, generating intense and diagnostic fragmentation when the ionization is performed at 70 eV; this is considered the standard ionization condition and has been used for creating many established databases, which are of great support in the analyte identification process. However, such an intense fragmentation often causes the loss of the molecular ion or more diagnostic ions, which can be detrimental for the identification of homologous series or isomers, as for instance fatty acids. To obtain this information chemical or soft ionization can be used, but dedicated ion sources and conditions are required. In this work, we explored different ionization voltages in GC–EI–MS to preserve the intensity of the molecular ion using a conventional quadrupole MS. Twenty, 30, 50, and 70 eV were tested using a mixture of fatty acid methyl esters standards. Intensity and repeatability of the most informative ions were compared. Twenty and 70 eV were then used to analyze the fatty acid composition of six different strains of mycobacteria. Two approaches were used for elaborating the data: (1) a single average spectrum of the entire chromatogram was derived, which can be considered (in terms of concept) as a direct EI–MS analysis; (2) the actual chromatographic separation of the compounds was considered after automatic alignment. The results obtained are discussed herein. [Figure not available: see fulltext.]. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature. [less ▲]

Detailed reference viewed: 28 (3 ULiège)
Full Text
Peer Reviewed
See detailExhaled human breath analysis in active pulmonary tuberculosis diagnostics by comprehensive gas chromatography-mass spectrometry and chemometric techniques
Beccaria, M.; Bobak, C.; Maitshotlo, B. et al

in Journal of Breath Research (2018), 13(1), 016005

Tuberculosis (TB) is the deadliest infectious disease, and yet accurate diagnostics for the disease are unavailable for many subpopulations. In this study, we investigate the possibility of using human ... [more ▼]

Tuberculosis (TB) is the deadliest infectious disease, and yet accurate diagnostics for the disease are unavailable for many subpopulations. In this study, we investigate the possibility of using human breath for the diagnosis of active TB among TB suspect patients, considering also several risk factors for TB for smokers and those with human immunodeficiency virus (HIV). The analysis of exhaled breath, as an alternative to sputum-dependent tests, has the potential to provide a simple, fast, non-invasive, and readily available diagnostic service that could positively change TB detection. A total of 50 individuals from a clinic in South Africa were included in this pilot study. Human breath has been investigated in the setting of active TB using the thermal desorption-comprehensive two-dimensional gas chromatography-time of flight mass spectrometry methodology and chemometric techniques. From the entire spectrum of volatile metabolites in breath, three machine learning algorithms (support vector machines, partial least squares discriminant analysis, and random forest) to select discriminatory volatile molecules that could potentially be useful for active TB diagnosis were employed. Random forest showed the best overall performance, with sensitivities of 0.82 and 1.00 and specificities of 0.92 and 0.60 in the training and test data respectively. Unsupervised analysis of the compounds implicated by these algorithms suggests that they provide important information to cluster active TB from other patients. These results suggest that developing a non-invasive diagnostic for active TB using patient breath is a potentially rich avenue of research, including among patients with HIV comorbidities. [less ▲]

Detailed reference viewed: 33 (2 ULiège)
Full Text
Peer Reviewed
See detailTowards the use of breath for detecting mycobacterial infection: A case study in a murine model
Franchina, Flavio ULiege; Mellors, T. R.; Aliyeva, M. et al

in Journal of Breath Research (2018), 12(2),

Detailed reference viewed: 29 (4 ULiège)
Full Text
Peer Reviewed
See detailThe volatile molecular profiles of seven Streptococcus pneumoniae serotypes
Mellors, T. R.; Rees, C. A.; Franchina, Flavio ULiege et al

in Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences (2018), 1096

Detailed reference viewed: 25 (2 ULiège)
Full Text
Peer Reviewed
See detailVolatile metabolic diversity of Klebsiella pneumoniae in nutrient-replete conditions
Rees, C. A.; Nordick, K. V.; Franchina, Flavio ULiege et al

in Metabolomics (2017), 13(2),

Detailed reference viewed: 27 (2 ULiège)
Full Text
Peer Reviewed
See detailIn-pipette solid-phase extraction prior to flow-modulation comprehensive two-dimensional gas chromatography with dual detection for the determination of minor components in vegetable oils
Barp, L.; Franchina, Flavio ULiege; Purcaro, Giorgia ULiege et al

in Talanta (2017), 165

The present research is based on the development of an effective, environmentally-friendly and low-cost method for investigation of minor components in vegetable oils, exploiting the advantages of a ... [more ▼]

The present research is based on the development of an effective, environmentally-friendly and low-cost method for investigation of minor components in vegetable oils, exploiting the advantages of a miniaturized solid-phase extraction (SPE) and the potential of flow modulation (FM) comprehensive two-dimensional gas chromatography (GC×GC), coupled to a mass spectrometer (MS) and a flame ionization detector (FID). The initial sample preparation step was carried out using a miniaturized approach characterized by a SPE process in a Pasteur pipette. Then, the isolated fraction was injected into an FM GC×GC system. Tentative identification was carried out by means of MS spectral information, while quantification was carried out by using the FID data. Four different vegetable oil samples were analyzed using the proposed method, namely an extra virgin olive oil (EVO), a hazelnut oil (HO), a borage oil, and an EVO/HO mixture. © 2017 Elsevier B.V. [less ▲]

Detailed reference viewed: 23 (3 ULiège)