References of "Frère, Antoine"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailPEGylated and Functionalized Aliphatic Polycarbonate Polyplex Nanoparticles for Intravenous Administration of HDAC5 siRNA in Cancer Therapy
Frère, Antoine ULiege; Baroni, Alexandra; Hendrick, Elodie ULiege et al

in ACS Applied Materials and Interfaces (2017), 25(9(3)), 2181-2195

Guanidine and morpholine functionalized aliphatic polycarbonate polymers are able to efficiently deliver histone deacetylase 5 (HDAC5) siRNA into the cytoplasm of cancer cells in vitro leading to a ... [more ▼]

Guanidine and morpholine functionalized aliphatic polycarbonate polymers are able to efficiently deliver histone deacetylase 5 (HDAC5) siRNA into the cytoplasm of cancer cells in vitro leading to a decrease of cell proliferation were previously developed. To allow these biodegradable and biocompatible polyplex nanoparticles to overcome the extracellular barriers and be effective in vivo after an intravenous injection, polyethylene glycol chains (PEG750 or PEG2000) were grafted on the polymer structure. These nanoparticles, showed an average size of about 150 nm and a slightly positive zeta potential with complete siRNA complexation. Behavior of PEGylated and non-PEGylated polyplexes were investigated in the presence of serum, in terms of siRNA complexation (Fluorescence Correlation Spectroscopy), size (Dynamic Light Scattering and Single-Particle Tracking), interaction with proteins (Isothermal Titration Calorimetry) and cellular uptake. Surprisingly, both PEGylated and non-PEGylated formulations presented relatively good behavior in the presence of fetal bovine serum (FBS). Hemocompatibility tests showed no effect of these polyplexes on hemolysis and coagulation. In vivo biodistribution in mice was performed and showed a better siRNA accumulation at the tumor site for PEGylated polyplexes. However, cellular uptake in protein-rich conditions showed that PEGylated polyplex lost their ability to interact with biological membranes and enter into cells, showing the importance to perform in vitro investigations in physiological conditions closed to in vivo situation. In vitro, the efficiency of PEGylated nanoparticles decreases compared to non-PEGylated particles, leading to the loss of the antiproliferative effect on cancer cells. [less ▲]

Detailed reference viewed: 85 (18 ULiège)
Peer Reviewed
See detailPolymeric Nanoparticles as siRNA Drug Delivery System for Cancer Therapy: The Long Road to Therapeutic Efficiency
Frère, Antoine ULiege; Evrard, Brigitte ULiege; Mottet, Denis ULiege et al

in Holban, Alina Maria; Grumezescu, Alexandru (Eds.) Nanoarchitectonics for Smart Delivery and Drug Targeting (2016)

Polyplexes are nanoparticles composed of small-interfering RNA (siRNA) and natural or synthetic polymers. To meet the challenge of gene therapy and deliver siRNA into the cytoplasm of target cells ... [more ▼]

Polyplexes are nanoparticles composed of small-interfering RNA (siRNA) and natural or synthetic polymers. To meet the challenge of gene therapy and deliver siRNA into the cytoplasm of target cells, several barriers must be overcome. In this chapter, the main steps, from the formulation of polyplexes to the efficient release of the siRNA into the cytoplasm of cancer cells, are described, taking into account the different strategies used to overcome the obstacles linked to the formulation of this type of nanovector. To allow a parenteral administration of the nanocolloids, the polyplex production methods should result in identical, stable, and reproducible nanostructures. Charge interactions occur between the anionic siRNA and the cationic/amphiphilic polymer. Once in the blood circulation, polyplexes must keep their physical stability. The positively charged surface can cause aggregation of the nanoparticles with plasma proteins, as well as complement activation and recognition by the mononuclear phagocytic system, with a consequent reduction of their pharmacological activity. Polyethylene glycol (PEG) can be added on the surface of the nanovectors to confer “the stealth” properties and increase plasma half-life. Then, particles have to preferentially accumulate in the tumor tissue following an active or passive targeting. Endocytosis process enables the polyplex cellular uptake, but some strategies like “the proton sponge effect” have to be used to allow the escape of the nanovectors from the cellular endosomes. Once released into the cytoplasm, polymer and siRNA must dissociate for an effective degradation of the targeted mRNA, leading finally to a decrease of the corresponding protein. [less ▲]

Detailed reference viewed: 196 (21 ULiège)
Full Text
Peer Reviewed
See detailCapillary electrophoresis method to determine siRNA complexation with cationic liposomes
Furst, Tania ULiege; Bettonville, Virginie ULiege; Farcas, Elena ULiege et al

in Electrophoresis (2016)

Small interfering RNA (siRNA) inducing gene silencing has great potential to treat many human diseases. To ensure effective siRNA delivery, it must be complexed with an appropriate vector, generally ... [more ▼]

Small interfering RNA (siRNA) inducing gene silencing has great potential to treat many human diseases. To ensure effective siRNA delivery, it must be complexed with an appropriate vector, generally nanoparticles. The nanoparticulate complex requires an optimal physiochemical characterization and the complexation efficiency has to be precisely determined. The methods usually used to measure complexation are gel electrophoresis and RiboGreen® fluorescence-based assay. However, those approaches are not automated and present some drawbacks such as the low throughput and the use of carcinogenic reagents. The aim of this work is to develop a new simple and fast method to accurately quantify the complexation efficiency. In this research, capillary electrophoresis (CE) was used to determine the siRNA complexation with cationic liposomes. The short-end injection mode applied enabled siRNA detection in less than 5 min. Moreover, the CE technique offers many advantages compared to the other classical methods. It is automated, does not require sample preparation and expensive reagents. Moreover, no mutagenic risk is associated to CE approach since no carcinogenic product is used. Finally, this methodology can also be extended to the characterization of other types of nanoparticles encapsulating siRNA, such as cationic polymeric nanoparticles. [less ▲]

Detailed reference viewed: 105 (31 ULiège)
See detailPegylated Polyplexes Based On HDAC5 siRNA And Aliphatic Polycarbonate Polymers For An Anticancer Therapy
Frère, Antoine ULiege; Baroni, Alexandra; Peulen, Olivier ULiege et al

Poster (2015, December)

Detailed reference viewed: 42 (6 ULiège)
See detailPegylated Polyplexes Based On HDAC5 siRNA And Aliphatic Polycarbonate Polymers For An Anticancer Therapy
Frère, Antoine ULiege; Baroni, Alexandra; Peulen, Olivier ULiege et al

Poster (2015, November 23)

Detailed reference viewed: 23 (10 ULiège)
See detailPolyplex Based on Polycarbonate Polymers for an Efficient Delivery of HDAC5 and HDAC7 siRNA
Frère, Antoine ULiege; Tempelaar, Sarah; Peulen, Olivier ULiege et al

Poster (2015, June 02)

Detailed reference viewed: 22 (5 ULiège)
Full Text
Peer Reviewed
See detailImpact of the Structure of Biocompatible Aliphatic Polycarbonates on siRNA Transfection Ability
Frère, Antoine ULiege; Kawalec, Michal; Tempelaar, Sarah et al

in Biomacromolecules (2015)

RNAi therapeutics are promising therapeutic tools that have sparked the interest of many researchers. In an effort to provide a safe alternative to PEI, we have designed a series of new guanidinium and/or ... [more ▼]

RNAi therapeutics are promising therapeutic tools that have sparked the interest of many researchers. In an effort to provide a safe alternative to PEI, we have designed a series of new guanidinium and/or morpholino functionalized biocompatible and biodegradable polycarbonate vectors. The impact of different functions (morpholino-, guanidinium-, hydrophobic groups), of the architecture (linear homopolymer to dumbbell-shape) and of the molecular weight of these copolymers on their capacity to form polyplexes and to decrease the expression of two epigenetic regulators of gene expression, HDAC7 and HDAC5 was evaluated. The use of one of these polymers combining morpholine and guanidine functions at the ratio >1 and hydrophobic trimethylene carbonate groups showed a significant decrease of mRNA and protein level in HeLa cells, similar to PEI. These results highlight the potential of polycarbonate vectors for future in vivo application as an anti-cancer therapy. [less ▲]

Detailed reference viewed: 96 (31 ULiège)
See detailPolyplex based on polycarbonate polymers for an efficient delivery of HDAC5 and HDAC7 siRNA
Frère, Antoine ULiege; Evrard, Brigitte ULiege; Mespouille, Laetitia et al

Conference (2014, December 12)

Detailed reference viewed: 21 (5 ULiège)
Full Text
See detailPolyplex based on polycarbonate polymesr for an efficient delivery of an anti-angiogenic siRNA
Frère, Antoine ULiege; Tempelaar, Sarah; Peixoto, Paul ULiege et al

Conference (2014, August 28)

Detailed reference viewed: 37 (13 ULiège)
See detailImpact of the Structure of Biocompatible Aliphatic Polycarbonate on siRNA Transfection Ability
Frère, Antoine ULiege; Kawalec, Michal; Tempelaar, Sarah et al

Poster (2014, May 26)

Detailed reference viewed: 55 (22 ULiège)
See detailPolyplexes Targeting Angiogenesis in Cancer
Frère, Antoine ULiege; Peixoto, Paul ULiege; Kawalec, Michal et al

Poster (2014, April)

Detailed reference viewed: 34 (5 ULiège)
Full Text
See detailPolyplex Based On Polycarbonate Polymers For An Efficient Delivery Of An Anti-Angiogenic siRNA
Frère, Antoine ULiege; Kawalec, Michal; Tempelaar, Sarah et al

Poster (2014)

Detailed reference viewed: 20 (8 ULiège)
See detailThe intracellular fate of polycarbonate Polyplexes modulates the efficacy of siRNA
Frère, Antoine ULiege; Kawalec, Michal; Peixoto, Paul ULiege et al

Poster (2013, December)

Detailed reference viewed: 23 (5 ULiège)
Full Text
See detailCharacterization and transfection experiments of polyplexes targeting HDAC7
Frère, Antoine ULiege; Kawalec, Michal; Collard, Laurence ULiege et al

Poster (2012, October 22)

Detailed reference viewed: 47 (18 ULiège)