References of "Everaert, Nadia"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailCharacterization of fructans and dietary fibre profiles in raw and steamed vegetables
Kalala Bolokango, Gaetan ULiege; Kambashi Mutiaka, Bienvenu; Everaert, Nadia ULiege et al

in International Journal of Food Sciences & Nutrition (in press)

Dietary fiber (DF) has many positive effects on human health associated with its functionality in the gastrointestinal tract. These benefits vary according to the type of DF. Vegetables can be a natural ... [more ▼]

Dietary fiber (DF) has many positive effects on human health associated with its functionality in the gastrointestinal tract. These benefits vary according to the type of DF. Vegetables can be a natural source of DF in the diet. However, to provide adequate nutritional advice, the content and profile of their various DF types must be characterized. This study aimed to determine the DF profile of 29 vegetables cultivated in Wallonia (Belgium) and the impact of steaming on these profiles. Using a combination of enzymatic, gravimetric and chromatographic methods, fructans, total dietary fiber (TDF), low- and high-molecular-weight soluble dietary fiber (SDF), and insoluble dietary fiber (IDF) were analyzed. Results show that the DF content varies considerably among the 29 investigated vegetable varieties and species, but the influence of steaming is limited to a shift from IDF to high-molecular-weight SDF for 18 of the 29 tested vegetables, while fructans are preserved with not actual reduction in the DP. [less ▲]

Detailed reference viewed: 53 (11 ULiège)
Full Text
Peer Reviewed
See detailDifferent sources of resistant starch in vitro show contrasting fermentation and SCFA profiles
Leblois, Julie ULiege; Bindelle, Jérôme ULiege; Everaert, Nadia ULiege

Poster (2018, August)

Resistant starch (RS) is well known to be fermented in the caecum and the colon of animals, increasing the production of short-chain fatty acids (SCFA), especially butyrate. The latter is health-promoting ... [more ▼]

Resistant starch (RS) is well known to be fermented in the caecum and the colon of animals, increasing the production of short-chain fatty acids (SCFA), especially butyrate. The latter is health-promoting, exhibiting anti-inflammatory effects on the gut and hence it has been postulated that dietary strategies should aim for an increased intestinal butyrate production in pig production. In this study, five different purified sources of RS (high-amylose maize, potato and pea starches) have been tested in vitro for their fermentation kinetics and SCFA profiles as a preliminary step to include one of these substrates in the diet of sows to modulate intestinal microbiota and fermentation patterns. Briefly, after an in vitro hydrolysis with porcine pepsin and pancreatin, undigested residues recovered by dialysis (1,000 kD) were fermented in vitro for 72 hours in a gas test using sows faeces as microbial inoculum. Six vials per RS source were fermented, 3 were used for determination of SCFA profiles at five consecutive time-points and 3 for the fermentation kinetics based on the monitoring of gas volume at regular intervals. SCFA production and profile was measured by high performance liquid chromatography, while fermentation kinetics was mathematically modelled to allow proper comparison between RS sources. All statistical analyses were performed with the MIXED procedure of SAS and repeated measurements for SCFA. All investigated parameters were influenced (p<0.05) by the RS source. Pea starch showed the highest butyrate level at each time point (>15% of total SCFA from 12 hours after the beginning of the fermentation onwards) and exhibited an extensive and rapid fermentation (highest final gas volume (A) and gas production rate and lowest time to reach A/2) while high amylose maize produced the lowest butyrate proportion (<6% of the total SCFA) during the slowest and lowest fermentation. Therefore, pea starch appears to be the most promising to be used in pig nutrition to modulate intestinal fermentation. [less ▲]

Detailed reference viewed: 17 (2 ULiège)
Full Text
Peer Reviewed
See detailMultigenerational effects of a reduced balanced protein diet during the rearing and laying period of broiler breeders. 2. Zootechnical performance of the F1 broiler offspring
Lesuisse, Jens; Schallier, Seline; Li, Congcong et al

in Poultry Science (2018), 97(5), 1666-1676

Several studies in mammals focused on the maternal programming of the metabolism by epigenetic mechanisms, while currently, the consequences of a maternal dietary treatment on the offspring performance of ... [more ▼]

Several studies in mammals focused on the maternal programming of the metabolism by epigenetic mechanisms, while currently, the consequences of a maternal dietary treatment on the offspring performance of farm animals are of particular interest for commercial purpose. In the present study, we investigated if the zootechnical performance of the progeny was altered by a maternal dietary treatment, being a lower dietary crude protein (CP) of the grandparent and/or parent generation. The multigenerational effects of a reduced maternal CP content were investigated by reducing the dietary CP level by 25% in rearing and laying diets of pure line A breeders. The F0 generation breeders were fed either control (C) or reduced balanced protein (RP) diets. The F1 breeder generation was constructed by dividing the F0 female progeny again over a C or RP diet, resulting in 4 dietary treatments in the F1 generation: C/C, C/RP, RP/C, and RP/RP (letters indicating the diets in, respectively, F0 and F1 generations). The offspring performance was evaluated by a zootechnical and nitrogen retention trial on C and low-protein (LP) broiler diets. For the C broiler diet, the C/RP and RP/RP offspring were characterized by a higher BW from d 35 until d 42 compared to the C/C progeny, whereas the RP/C offspring had an intermediate BW that did not differ from the other groups. A tendency (P = 0.067) towards a better nitrogen retention was observed for the offspring of breeders that received the RP diets in F0 and/or F1 generation compared to the C/C progeny. For the LP broiler diet, the C/RP (P = 0.021) and RP/C (P = 0.001) offspring had a higher BW compared to the C/C progeny during the entire grow-out period. In addition, the C/RP offspring were characterized by a lower FCR from d 28 onwards (P = 0.021). In conclusion, dietary treatments imposed on mother hens can have direct effects on the next generation, as well as indirect effects on multiple generations. [less ▲]

Detailed reference viewed: 5 (1 ULiège)
Full Text
Peer Reviewed
See detailReview on the effects of potential prebiotics on controlling intestinal enteropathogens Salmonella and Escherichia coli in pig production
Tran, Thi Hanh Tham ULiege; Everaert, Nadia ULiege; Bindelle, Jérôme ULiege

in Journal of Animal Physiology and Animal Nutrition (2018), 102

Salmonella enterica serotypes (Salmonella sp.) are the second cause of bacterial foodborne zoonoses in humans after campylobacteriosis. Pork is the third most important cause for outbreak-associated ... [more ▼]

Salmonella enterica serotypes (Salmonella sp.) are the second cause of bacterial foodborne zoonoses in humans after campylobacteriosis. Pork is the third most important cause for outbreak-associated salmonellosis, and colibacillosis is the most important disease in piglets and swine. Attachment to host cells, translocation of effector proteins into host cells, invasion and replication in tissues are the vital virulence steps of these pathogens that help them to thrive in the intestinal environment and invade tissues. Feed contamination is an important source for Salmonella infection in pig production. Many on-farm feeding strategies intervene to avoid the introduction of pathogens onto the farm by contaminated feeds or to reduce infection pressure when pathogens are present. Among the latter, prebiotics could be effective at protecting against these enteric bacterial pathogens. Nowadays, a wide range of molecules can potentially serve as prebiotics. Here, we summarize the prevalence of Salmonella sp. and Escherichia coli in pigs, understanding of the mechanisms by which pathogens can cause disease, the feed related to pathogen contamination in pigs and detail the mechanisms on which prebiotics are likely to act in order to fulfil their protective action against these pathogens in pig production. Many different mechanisms involve the inhibition of Salmonella and E. coli by prebiotics such as coating the host surface, modulation of intestinal ecology, downregulating the expression of adhesin factors or virulence genes, reinforcing the host immune system. [less ▲]

Detailed reference viewed: 18 (3 ULiège)
Full Text
Peer Reviewed
See detailEffet de la cuisson sur le profil en fibres de quelques légumes tropicaux
Kalala Bolokango, Gaetan ULiege; KAMBASHI, Bienvenu; Everaert, Nadia ULiege et al

Poster (2017, December 13)

Outre le monde occidental, les pays africains dont la population a souffert de malnutrition intra-utérine sont également exposés au fléau de l’obésité à l’âge adulte en raison d’effets de programmation de ... [more ▼]

Outre le monde occidental, les pays africains dont la population a souffert de malnutrition intra-utérine sont également exposés au fléau de l’obésité à l’âge adulte en raison d’effets de programmation de long terme induits par les carences dans le jeune âge. La consommation de fibres alimentaires (DF) demeure indispensable pour la modulation du microbiote intestinal afin de prévenir cette maladie et les pathologies associées. Les légumes et les fruits sont des principales sources naturelles de DF. . En Afrique tropicale humide et sub-humide, les légumes feuilles sont fortement consommés par les populations rurales, tandis que les populations urbaines modifient leur régime alimentaire en l’ « occidentalisant ». Afin de mieux comprendre comment les changements alimentaires modifient les modes de consommation des fibres alimentaires, face à la déficience d’information dans la littérature à ce sujet, il convient de caractériser le profil en fibres de légumes méconnus et l’impact de la cuisson sur celui-ci. [less ▲]

Detailed reference viewed: 16 (3 ULiège)
Full Text
Peer Reviewed
See detailFeeding broiler breeders a reduced balanced protein diet during the rearing and laying period impairs reproductive performance but enhances broiler offspring performance
Lesuisse, Jens; Li, Congcong; Schallier, Seline et al

in Poultry Science (2017), 96(11), 3949-3959

Mammalian studies have shown that nutritional constraints during the perinatal period are able to program the progeny (metabolism, performance). The presented research aimed to investigate if broiler ... [more ▼]

Mammalian studies have shown that nutritional constraints during the perinatal period are able to program the progeny (metabolism, performance). The presented research aimed to investigate if broiler breeders and their offspring performance could be influenced by reducing the dietary crude protein (CP) level with 25%. A total of 160 day-old pure line A breeder females were randomly divided over 2 dietary treatments. The control group was fed commercial diets, whereas the reduced balanced protein (RP) breeders received an isoenergetic diet that was decreased with 25% in dietary CP and amino acid during their entire lifespan. The RP birds required an increased feed allowance, varying between 3 and 15%, to meet the same BW goals as their control fed counterparts. The difference in feed allocations and reduction of the dietary CP level resulted in a net protein reduction varying between 14 and 23%. At wk 27 and 40, the body composition of the breeders was changed as a result of the dietary treatment. At both ages, the proportional abdominal fat pad weight of the RP breeders was increased (P < 0.001), whereas the proportional breast muscle weight was only higher at wk 27 in the control group compared to the RP group (P < 0.001). Egg weight (P < 0.001) and egg production (P < 0.001) was decreased for the RP fed birds. The lower dietary CP level reduced the proportional albumen weight of the RP eggs (P = 0.006). Male offspring from RP breeders were characterized by an increase in BW from 28 d until 35 d of age (P = 0.015). Moreover, female progeny of RP breeders showed a reduced FCR (P = 0.025), whereas male progeny showed a tendency (P = 0.052) towards a lower FCR at 5 wk of age. In conclusion, lowering dietary CP levels in rearing and laying phase of breeders had a negative effect on breeder performance but enhanced live performance of the offspring. [less ▲]

Detailed reference viewed: 15 (1 ULiège)
Full Text
See detailImpact of 3’-­‐sialyllactose and Bifidobacterium crudilactis on infant feces microbial composition and virulence modulation of Escherichia coli O157:H7, using the SHIME® gastrointes6nal model
Bondue, Pauline ULiege; Lebrun, Sarah ULiege; Crevecoeur, Sébastien ULiege et al

Poster (2017, June)

Bifidobacterium crudilactis, a bacterial species from bovine origin, growths on bovine milk oligosaccharides (BMO). Cell free spent media (CFSM) from B. crudilactis and 3’-sialyllactose (3’SL), a major ... [more ▼]

Bifidobacterium crudilactis, a bacterial species from bovine origin, growths on bovine milk oligosaccharides (BMO). Cell free spent media (CFSM) from B. crudilactis and 3’-sialyllactose (3’SL), a major BMO, modulated Escherichia coli O157:H7 virulence gene expressiona. In this study, the SHIME® gastrointestinal model was inoculated with 4 different treatments: 3’SL (ttm1), B. crudilactis (ttm2), 3’SL and B. crudilactis (ttm3) and CFSM from 3’SL and B. crudilactis culture (ttm4). In each section of the colon, samples were collected and analysed for short chain fatty acid (SCFA) concentration using HPLC, microbial populations using 16S rDNA metagenetic analysis and correlated with E. coli O157:H7 virulence gene expression. The results showed that SCFA levels were stable during the experiments. Metagenetic analysis showed a microbial diversity in transverse (TC) and descending colon (DC) close to feces, dominated by Bacteroides, Prevotella and Fusobacterium, while the ascending colon (AC) showed a different microbial diversity dominated by Veillonella. Ttm4 and ttm2 induced mainly a down-regulation of virulence genes: fliC in DC with ttm4, and luxS, stx1, qseA in AC, DC or TC with ttm2. Ttm1 also showed a down-regulation of fliC in DC, similar to the one observed with ttm4, but this was associated with an up-regulation of fliC and stx1 in AC or TC. Finally, ttm3 showed slight upregulation of ler, fliC and qseA in AC. These results show that ttm4 and ttm2 might have a positive effect against virulence expression of E. coli O157:H7. However, this trend has to be validated with the further replicates on the SHIME® system. [less ▲]

Detailed reference viewed: 18 (7 ULiège)
Full Text
Peer Reviewed
See detailA review on early gut maturation and colonization in pigs, including biological and dietary factors affecting gut homeostasis
Everaert, Nadia ULiege; Van Cruchten, Steven; Weström, Björn et al

in Animal Feed Science & Technology (2017)

Detailed reference viewed: 30 (7 ULiège)
Full Text
Peer Reviewed
See detailEffect of feed enzymes on digestibility and growth in weaned pigs: A systematic review and meta-analysis
Torres-Pitarch, Alberto; Hermans, David; Manzanilla, Edgar Garcia et al

in Animal Feed Science & Technology (2017), 233

Supplementation of post-weaning diets with exogenous enzymes has been suggested to control post-weaning syndrome, by compensating for the under-developed endogenous enzyme secretory capacity and ... [more ▼]

Supplementation of post-weaning diets with exogenous enzymes has been suggested to control post-weaning syndrome, by compensating for the under-developed endogenous enzyme secretory capacity and increasing nutrient digestibility in newly weaned pigs. The effect of in-feed enzymes in improving gut maturation, growth and/or health in weaned piglets is not always consistent. A systematic review and a meta-analysis were therefore conducted to determine which exogenous enzymes are most consistent in improving piglet growth and digestibility when supplemented to post-weaning diets. The mean difference effect of enzyme supplementation on growth and digestibility of dry matter (DM), gross energy (GE), crude protein (CP), and P digestibility was calculated for each study and this was used as the effect size estimate in the meta-analysis. The impact of feed enzyme supplementation on intestinal health and bone mineralization was also discussed where information was available in the literature. From a total of 90 studies included in the metaanalysis, G:F was improved in 55, remained un-changed in 28 and deteriorated in 7, in response to enzyme supplementation. Average daily gain, average daily feed intake (ADFI) and G:F was improved when phytase was supplemented in the diet. Phosphorous digestibility was increased with phytase supplementation while GE digestibility was reduced. Dietary phytase supplementation increased bone mineralization in pigs fed diets with a reduced Pcontent. Supplementation with multi-enzyme complexes increased DM and CP digestibility. In conclusion, the most consistent improvement in piglet growth, P digestibility and bone mineralization were found due to exogenous phytase supplementation. Supplementation with xylanase alone or in combination with β-glucanase had inconsistent effects on piglet growth and nutrient digestibility. The most consistent improvements in growth and nutrient digestibility due to supplementation with multi-enzymes complexes were found when mannanase and/or protease were included in the complex. [less ▲]

Detailed reference viewed: 61 (12 ULiège)
Full Text
Peer Reviewed
See detailModulation of piglets’ microbiota: differential effects by a high wheat bran maternal diet during gestation and lactation
Leblois, Julie ULiege; Massart, Sébastien ULiege; Li, Bing et al

in Scientific Reports (2017)

Reaching a beneficial intestinal microbiota early in life is desirable for piglets, as microbiota will impact their future health. One strategy to achieve this is the addition of prebiotics to sows’ diet ... [more ▼]

Reaching a beneficial intestinal microbiota early in life is desirable for piglets, as microbiota will impact their future health. One strategy to achieve this is the addition of prebiotics to sows’ diet, as their microbiota will be transferred. Transmission of microbiota to the offspring occurs at birth and during lactation but a transfer might also occur during gestation. The objectives of this study were to determine whether and when (before and/or after birth) a maternal transfer of the microbiota occurs, and to observe the impact of wheat bran (WB) in sows’ diet on their faecal microbiota, their offspring’s microbiota and fermentation profile. Sequencing was performed on DNA extracted from umbilical cord blood, meconium, sows’ faeces and piglets’ colon content. Short-chain fatty acid production was determined in piglets’ distal gut. Different bacteria (mostly Proteobacteria, followed by Firmicutes) were found in the umbilical cord blood, suggesting a maternal transfer occurring already during gestation. Less butyrate was produced in the caecum of WB piglets and a lower concentration of valerate was observed in all intestinal parts of WB piglets. Maternal wheat bran supplementation affected microbiota of sows and piglets differently. [less ▲]

Detailed reference viewed: 77 (25 ULiège)
Full Text
Peer Reviewed
See detailAllelic variation in TLR4 is linked to resistance to Salmonella Enteritidis infection in chickens.
Li, Peng; Wang, Huihua; Zhao, Xingwang et al

in Poultry Science (2017)

Salmonella Enteritidis (SE) is a foodborne pathogen that negatively affects both animal and human health. Polymorphisms of the TLR4 gene may affect recognition by Toll-like receptor 4 (TLR4) of bacterial ... [more ▼]

Salmonella Enteritidis (SE) is a foodborne pathogen that negatively affects both animal and human health. Polymorphisms of the TLR4 gene may affect recognition by Toll-like receptor 4 (TLR4) of bacterial lipopolysaccharide (LPS), leading to differences in host resistance to pathogenic infections. The present study has investigated polymorphic loci of chicken TLR4 (ChTLR4) in ten chicken breeds, electrostatic potentials of mutant structures of TLR4, and a linkage analysis between allelic variation and survival ratio to infection with SE in specific-pathogen-free (SPF) White Leghorns. A total of 19 Single Nucleotide Polymorphisms (SNPs), of which 10 were novel, were found in chicken breeds. Seven newly identified amino acid variants (C68G, G674A, G782A, A896T, T959G, T986A, and A1104C) and previously reported important mutations (G247A, G1028A, C1147T, and A1832G) were demonstrated in the extracellular domain of the ChTLR4 gene. Significant changes in surface electrostatic potential of the ectodomain of TLR4, built by homology modeling, were observed at the Glu83Lys (G247A), Arg298Ser (A896T), Ser368Arg (A1104C), and Gln611Arg (A1832G) substitutions. Linkage analysis showed that one polymorphic locus G247A of TLR4 gene, common in all breeds examined, was significantly associated with increased resistance to SE in SPF White Leghorns chicks (log-rank P-value = 0.04). The genotypes from A1832G SNPs did not show statistically significant survival differences. This study has provided the first direct evidence that G247A substitution in ChTLR4 is associated with increased resistance to Salmonella Enteritidis. [less ▲]

Detailed reference viewed: 9 (3 ULiège)
Full Text
Peer Reviewed
See detailAlpha-lipoic acid impairs body weight gain of young broiler chicks via modulating peripheral AMPK.
Wang, Yufeng; Everaert, Nadia ULiege; Song, Zhigang et al

in Comparative Biochemistry and Physiology Part A : Molecular & Integrative Physiology (2017), 211

In mammals, the AMP-activated protein kinase (AMPK) pathways in the central and peripheral tissues coordinately integrate inputs from multiple sources to regulate energy balance. The present study was ... [more ▼]

In mammals, the AMP-activated protein kinase (AMPK) pathways in the central and peripheral tissues coordinately integrate inputs from multiple sources to regulate energy balance. The present study was aimed to explore the potential role of hepatic AMPK in the energy homeostasis of broiler chickens. Diets with 0, 0.05% or 0.1% alpha-lipoic acid (alpha-LA), a known AMPK inhibitor were provided to broiler chicks for 7days. As a result, alpha-LA supplementation decreased the relative growth rate of broiler chicks. Hepatic AMPKalpha2 mRNA levels were significantly upregulated by dietary alpha-LA, in concert with the increased phosphorylated AMPKalpha protein levels. In addition, hepatic FAS mRNA levels together with the malonyl-CoA to total CoA ester ratio were reduced by alpha-LA supplementation. Moreover, the hepatic phosphorylated glycogen synthase levels were increased resulting in a markedly decreased hepatic glycogen content. In conclusion, dietary alpha-LA supplementation decreased the in vivo hepatic glycogenesis and lipogenesis via stimulating hepatic AMPKalpha mRNA levels and the phosphorylated gene product. The stimulatory effect of alpha-LA on hepatic AMPK mRNA and pAMPKalpha protein levels together with our previous observations regarding its inhibitory effect on hypothalamic AMPK may have altered the energy balance and hence impaired body weight gain of broiler chicks. [less ▲]

Detailed reference viewed: 11 (4 ULiège)
Full Text
Peer Reviewed
See detailAttenuating Effect of Zinc and Vitamin E on the Intestinal Oxidative Stress Induced by Silver Nanoparticles in Broiler Chickens.
Song, Zhigang; Lv, Jiadong; Sheikhahmadi, Ardashir et al

in Biological Trace Element Research (2017)

Silver nanoparticles (AgNPs) have been increasingly used as antimicrobial and disinfectant. However, intestinal model studies have shown that AgNPs induce oxidative stress. Hence, this study aims to ... [more ▼]

Silver nanoparticles (AgNPs) have been increasingly used as antimicrobial and disinfectant. However, intestinal model studies have shown that AgNPs induce oxidative stress. Hence, this study aims to investigate the effects of dietary supplemental zinc (Zn) and vitamin E (VE; alpha-tocopherol acetate) on attenuating AgNP-induced intestinal oxidative stress in broiler chickens. The chickens were divided into two groups as follows: (1) control group fed with a corn-soybean meal basal diet and (2) nano group, received drinking water containing 1000 mg/kg AgNPs. All the nano-exposed birds were divided into six dietary treatment groups, namely, the basal diets supplemented with (1) 60 mg/kg Zn as ZnSO4, (2) 120 mg/kg Zn, (3) 100 mg/kg VE, (4) 200 mg/kg VE, (5) 60 mg/kg Zn and 100 mg/kg VE, and (6) 120 mg/kg Zn and 200 mg/kg VE. Results showed that the AgNPs significantly reduced the body weights of the broilers after 42 days of oral administration of AgNPs (P < 0.05), and this effect was not alleviated by any of the dietary treatments. The activity of superoxide dismutase (CuZn-SOD) increased in all the AgNP-treated birds (P < 0.05); however, CuZn-SOD did not increase in birds fed with basal diet supplemented with 200 mg/kg VE. In this treatment, the VE exerted an antioxidant effect to prevent the activation of the CuZn-SOD enzyme. Furthermore, supplementing Zn increased the activities of catalase and glutathione peroxidase in the jejunal mucosa (P < 0.05), which were accompanied with increased malondialdehyde levels (P < 0.05) in the broilers. AgNP exposure resulted in a significant messenger RNA (mRNA) upregulation of toll-like receptor 4 (TLR4) and TLR2-1 in the jejunal mucosa (P < 0.05). However, supplemental ZnVE did not reduce TLRs' mRNA expression, except for the diminished TLR2-1 mRNA levels in birds fed with basal diet supplemented with 120 mg/kg Zn and 200 mg/kg VE. We concluded that although dietary Zn and VE supplementation did not attenuate growth depression effect of AgNP, it however attenuates intestinal oxidative stress in AgNP-treated broiler chickens. [less ▲]

Detailed reference viewed: 31 (12 ULiège)
Full Text
Peer Reviewed
See detailLe profil en fibres alimentaires de légume modifie leur fermentation intestinale et la production d’acides gras à chaine courte dans modèle in vitro humain
Kalala Bolokango, Gaetan ULiege; Kambashi Mutiaka, Bienvenu ULiege; Njeumen, Patrick et al

Poster (2016, December 01)

La modulation du microbiote intestinal induite par les fibres alimentaires (DF)a des conséquences positives sur la santé, notamment sur l’obésité et ses comorbidités. La nature et l’ampleur des ... [more ▼]

La modulation du microbiote intestinal induite par les fibres alimentaires (DF)a des conséquences positives sur la santé, notamment sur l’obésité et ses comorbidités. La nature et l’ampleur des changements dépendent du type de fibres.Les légumes sont des sources privilégiées de DF, certaines espèces et variétés fournissant davantage de DF solubles (SDF), d’autres étant plus riches en DF insolubles (IDF). Contrairement aux SDF, les IDF sont souvent considérées comme peu fermentescibles, ne contribuant que faiblement à une modulation de l’écophysiologie intestinale. En opposition à cette hypothèse, pour explorer si l’impact potentiel sur la santé intestinale des légumes varie suivant leur nature, des légumes présentant des teneurs et des profils en DF différents ont été comparés dans un modèle in vitro de fermentation colique. [less ▲]

Detailed reference viewed: 86 (16 ULiège)
Full Text
See detailImpact of cooking and species on intestinal fermentation patterns of vegetables in a humanized in vitro model of the gastro-intestinal tract
Kalala Bolokango, Gaetan ULiege; Kambashi Mutiaka, Bienvenu ULiege; Njeumen Lemotio, Georges Patrick ULiege et al

Poster (2016, June 21)

Obesity and associated pathologies have dramatic consequences on patients’lives as well as high societal costs. Because of the role of intestinal dysbiosis and microbiota make-up on the pathogenesis of ... [more ▼]

Obesity and associated pathologies have dramatic consequences on patients’lives as well as high societal costs. Because of the role of intestinal dysbiosis and microbiota make-up on the pathogenesis of obesity, several strategies such as eating prebiotics and dietary fibre supplements are being investigated to reshape the intestinal microbial communities of obese patients. Beyond supplement, dietary fibre is supplied through plant ingredients in the meals. In the framework of the multidisciplinary research project Food4Gut, the use of vegetables rich in specific targeted dietary fiber, namely fructans, is being scrutinized for its ability to induce positive changes in the intestinal ecophysiology. Because expected effect might differ according the content in dietary fibre and fructans, the soluble:insoluble ratio, as well as the cooking of the vegetables, the fermentation patterns of several vegetables are being investigated in an dual in vitro model combining enzymatic hydrolysis to an in vitro fermentation step using faecal inoculums from humans, to evaluate the performance of gut microbiota, modulation of metabolic functions. Six vegetables were sampled in triplicates (N=3) and steamed for 20 to 30 min.: Jerusalem artichoke, salsify, asparagus, pumpkin, fennel and swede. They were chosen because they display a variety of contents in fructans, soluble (SDF) and insoluble dietaryfibre (IDF). Steamed vegetable samplesand burgers from local fast food restaurants (negative control) were hydrolyzed in vitro why porcine pepsin and pancreatin to mimic digestion in the upper gut and indigested fiber residues were recover using a 6kDa dialysis membrane.Subsequently, in vitro fermentation is being run with independent fecal inoculums from obese and lean patients (N=4). Fermentation kinetics over 24h as well as short-chain fatty acid production and profiles will be compared according to the individual donor and the vegetable species and multivariate analysis will be used to explore the relationships between donor, vegetable species and composition and fermentation patterns. [less ▲]

Detailed reference viewed: 72 (31 ULiège)
Full Text
See detailEffects of new potential prebiotics on Salmonella Thyphimurium in pigs
Tran, Thi Hanh Tham; Everaert, Nadia ULiege; Boudry, Christelle et al

Conference (2016, April 15)

Detailed reference viewed: 63 (28 ULiège)