References of "Ectors, Fabien"
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See detailPrinciples of Anatomy and Physiology, 15 Edition
Ectors, Fabien ULiege; Vitte, Elisabeth; Bain, Renan et al

Book published by DeBoeck Supérieur - 5e édition (2018)

Cette cinquième édition du Tortora, Anatomie et physiologie, entièrement mise à jour, permet au lecteur de s'initier à l'anatomie et à la physiologie et d'acquérir de solides bases scientifiques. Le ... [more ▼]

Cette cinquième édition du Tortora, Anatomie et physiologie, entièrement mise à jour, permet au lecteur de s'initier à l'anatomie et à la physiologie et d'acquérir de solides bases scientifiques. Le TORTORA propose : - une présentation de l'homéostasie, définie comme l'état d'équilibre physiologique dynamique de l'organisme ; - des liens entre la structure et la fonction qui facilitent l'apprentissage de l'anatomie et de la physiologie ; - des schémas des mécanismes de régulation de l'homéostasie ; - des illustrations encore plus nombreuses, dont le graphisme a été amélioré, et de nouvelles photographies. Il aborde des sujets aussi importants que le développement embryonnaire, qui aide le lecteur à comprendre, par exemple, la « logique » de l'anatomie humaine ; le vieillissement, qui rappelle que l'anatomie et la physiologie ne sont pas statiques ; l'exercice, qui explique les effets de l'activité physique sur les structures anatomiques et les fonctions physiologiques. [less ▲]

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See detailONE-STEP VITRIFICATION OF MURINE EMBRYOS CHALLENGES CURRENT PARADIGMS OF CRYOBIOLOGY
Ectors, Fabien ULiege; Vanderzwalmen, Pierre ULiege; Dupuis, Nadine et al

in Rawson, D.M. (Ed.) Cryobiology (2018, July 10)

Cryopreservation of embryos is amongst the most powerful tools for preserving the genetics of laboratory animals. Vitrification is widely recognized as more efficient than slow freezing e.g. in human ... [more ▼]

Cryopreservation of embryos is amongst the most powerful tools for preserving the genetics of laboratory animals. Vitrification is widely recognized as more efficient than slow freezing e.g. in human assisted reproduction technologies and for preserving murine embryos. Unfortunately, current vitrification procedures require multiple pre-cooling and post-warming exposure steps to dedicated solutions to reach maximum effectiveness, which appears difficult to deal with when many embryos are cryopreserved in one single session. To help solving this issue, we have developed a one-step embryo vitrification procedure. Briefly, embryos are exposed to a unique –chemically defined- vitrification solution before plunging in the liquid nitrogen. Subsequent warming is performed by immersing the vitrified embryos directly into the culture medium. Murine embryos at the zygote, two cells and morula stages have undergone our one-step procedure either under aseptic or non-aseptic conditions. After warming, direct in vitro survival, development to the blastocyst stage and in vivo development to birth were recorded as endpoints. Short exposure times to the vitrification solution (less than 60 seconds) before cooling and direct warming into culture medium led to results equivalent or better than after classical vitrification. Longer exposure times to the vitrification solution (between 90 and 150 seconds) decreased efficiency. These results demonstrate that intracellular vitrification after our one-step procedure occurs by combined effects of fast cooling (or warming) and cell dehydration, with minimal, if any, ingress of cryoprotectants. The absence of deleterious effect of warming directly into the culture medium and the relatively low sensitivity to thermal inertia (aseptic vs non-aseptic) of the carrier is a confirmation thereof. Consequently, beyond bringing a methodological simplification without any loss of efficiency, our patented one-step vitrification procedure dramatically lowers if not suppresses intracellular concentration of cryoprotectants and associated toxicity, thereby challenging some commonly accepted concepts of cryobiology. [less ▲]

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See detailMéthode de vitrification en une étape
Connan, Delphine; Ectors, Fabien ULiege; Vanderzwalmen, Pierre ULiege et al

Patent (2018)

Ce brevet rapporte la description et les résultats d'une nouvelle méthode de vitrification cellulaire dont le conditionnement repose sur une seule et brève étape d'exposition des cellules à un milieu ... [more ▼]

Ce brevet rapporte la description et les résultats d'une nouvelle méthode de vitrification cellulaire dont le conditionnement repose sur une seule et brève étape d'exposition des cellules à un milieu vitrifiant avant le refroidissement brutal dans l'azote liquide. Il en résulte une (quasi-)absence de pénétration intracellulaire de cryoprotecteurs, le conditionnement reposant essentiellement sur une déshydratation. Le réchauffement est effectué lui aussi en une seule étape, sans nécessité de passer par des étapes d'équilibration dans des solutions hypertoniques. Cette méthode remet en cause les paradigmes de la vitrification, qui postulent la nécessité d'une équilibration progressive des milieux extra et intracellulaires préalables au refroidissement et durant le réchauffement, et où des cryoprotecteurs pénètrent dans la cellule. [less ▲]

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See detailLa vitrification en une seule étape d’embryons murins définit de nouveaux standards de cryopréservation
Ectors, Fabien ULiege; Vanderzwalmen, Pierre ULiege; dupuis, Nadine et al

Poster (2018, June 13)

La cryopréservation d’embryons est un des outils les plus efficaces, économiques et utiles au niveau éthique pour préserver indéfiniment la génétique des animaux de laboratoire. Les bénéfices liés à son ... [more ▼]

La cryopréservation d’embryons est un des outils les plus efficaces, économiques et utiles au niveau éthique pour préserver indéfiniment la génétique des animaux de laboratoire. Les bénéfices liés à son utilisation sont nombreux, et incluent la réduction des coûts associés à la pérennisation de lignées, la limitation de l’apparition et de la dissémination de mutations non voulues, la facilité et la sécurité pour les transferts internationaux, et la réduction du nombre d’animaux à élever en captivité. La vitrification a été démontrée comme étant plus efficace que la congélation lente en procréation médicalement assistée humaine, où elle constitue maintenant la méthode de référence. Il en va de même pour la cryopréservation des embryons de souris, où la vitrification préserve mieux l’intégrité de la chromatine, réduit la pénétration intracytoplasmique d’agents cryoprotecteurs potentiellement toxiques, et in fine permet une meilleure survie et un meilleur développement après réchauffement que la congélation lente. Cependant, pour être efficaces, les méthodes actuelles de vitrification nécessitent plusieurs étapes d’exposition des embryons à des solutions spécifiques avant le refroidissement, mais aussi au cours de leur réchauffement ultérieur. Cette relative complexité peut s’avérer difficile à gérer de manière optimale quand un grand nombre d’embryons doivent être traités au cours d’une même session. Nous avons développé et breveté une technologie de vitrification d’embryons en une étape (« one-step ») qui est aussi efficace que les meilleures méthodes de vitrification multi-étapes. De plus, nos milieux sont chimiquement définis (sans sérum ou autre composant biologique non défini), et des supports permettant la vitrification aseptique peuvent être utilisés sans perte de rendement. Notre technologie de vitrification one-step d’embryons de rongeurs répond ainsi aux problèmes d’ergonomie liés aux méthodes classiques de vitrification, et fournit ainsi aux scientifiques une solution efficace, biologiquement sûre et facile à utiliser pour la cryopréservation d’embryons de rongeurs. Elle rend ainsi l’efficacité de la vitrification plus aisément applicable aux rongeurs de laboratoire, contribuant ainsi à la réduction du nombre d’animaux nécessaires à la pérennisation et à la diffusion de lignées ou de colonies utiles. [less ▲]

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See detailOne-step vitrification of murine embryos redefines cryopreservation standards
Ectors, Fabien ULiege; Vanderzwalmen, Pierre ULiege; Dupuis, Nadine et al

Poster (2018, April 26)

Cryopreservation of embryos is amongst the most powerful and efficient tools for indefinitely preserving the genetics of laboratory animals. The ensuing benefits are numerous and include reduction of ... [more ▼]

Cryopreservation of embryos is amongst the most powerful and efficient tools for indefinitely preserving the genetics of laboratory animals. The ensuing benefits are numerous and include reduction of costs associated to strain perpetuation, limitation of mutations occurrence and spreading, ease and safety of transnational shipping, and reduction of live animal husbandry. It has been demonstrated that vitrification is more efficient than slow freezing in human assisted reproduction, where it stands now as the gold standard. This is equally true for murine embryos, where vitrification has been shown to better preserve chromatin integrity, induce lower intracellular ingress of cryoprotectants and ultimately yield better embryo survival and development than slow freezing. Beside these benefits, current vitrification procedures require multiple pre-cooling and post-warming exposure steps to dedicated solutions to reach maximum effectiveness, which appears difficult to deal with when many embryos must be cryopreserved in one single session. We have developed and patented a unique one-step embryo vitrification procedure which is as efficient as the best multi-step vitrification methods. Moreover, our media are chemically defined (no serum nor undefined biological component), and aseptic vitrification carriers can be used without any yield loss. Our one-step vitrification kits and media for rodents (VitriMice™, VitriCell) address the poor ergonomy issues of classical vitrification, providing scientists with efficient, biologically safe and user-friendly solutions for embryo cryopreservation. Consequently, our one-step vitrification technology improves efficiency and applicability of cryopreservation for laboratory rodents, thereby contributing to the reduction of the number of live animals required to perpetuate and spread useful strains and colonies. [less ▲]

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See detailVitriCell: The cool way from academic research to company foundation
Connan, Delphine; Ectors, Fabien ULiege; Grobet, Luc ULiege

Poster (2018, March 06)

Poster describing the pathway from academic research to the creation of the Vitricell spin-off company

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See detailRelationship between follicular volume and oocyte competence, blastocyst development and live-birth rate: optimal follicle size for oocyte retrieval
Wirleitner, Barbara; Okhowat, Jasmin; Vi Stejnová, Lucie et al

in Ultrasound in Obstetrics and Gynecology (2018), 51

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See detailCryopreservation of chicken primordial germ cells by vitrification and slow-freezing: a comparative study
Tonus, Céline ULiege; Connan, Delphine ULiege; Waroux, Olivier ULiege et al

in Theriogenology (2017), 88

In the present study, we compare a classical slow freezing method and an aseptic vitrification technique to cryopreserve a stable Primordial Gem Cells (PGCs) line issued from the Ardennaise chicken breed ... [more ▼]

In the present study, we compare a classical slow freezing method and an aseptic vitrification technique to cryopreserve a stable Primordial Gem Cells (PGCs) line issued from the Ardennaise chicken breed. Viability immediately after warming was close to 80% and did not differ between the two cryopreservation methods. Proliferation tended to be slower for both cryopreservation methods compared to controls, but the difference was significant only for vitrification. No difference was found between the two methods after flow cytometry analysis of SSEA-1 expression and RT-PCR on several factors related to PGCs phenotype. After one week in culture, all cryopreserved cells reached controls main morphological and expanding (viability/proliferation) features. However, slow freezing generated more unwanted cells clusters than vitrification. After injection of the PGCs into recipient embryos, vitrified PGCs showed a clear, yet not significant, tendency to colonize the gonad at a higher rate than slow frozen PGCs. Slow freezing in cryovials remains simple, inexpensive and less technically demanding than vitrification. Nevertheless, the intrinsic advantages of our aseptic vitrification method and the present study suggest that this should be considered as safer than classical slow freezing for cryopreserving chicken PGCs. [less ▲]

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See detailVitrification: Research and state-of-the-art in humans and animals
Ectors, Fabien ULiege

Scientific conference (2016, December 09)

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See detailVitriCell: the safest key for cryopreserving your valuable cells
Connan, Delphine; Ectors, Fabien ULiege; Vanderzwalmen, Pierre ULiege et al

Poster (2016, December 08)

VITRICELL is a future spin-off company aiming at providing standard and customized solutions for safe and efficient cryopreservation of valuable and sensible eukaryotic cells. Developed in the Embryology ... [more ▼]

VITRICELL is a future spin-off company aiming at providing standard and customized solutions for safe and efficient cryopreservation of valuable and sensible eukaryotic cells. Developed in the Embryology unit of the Faculty of Veterinary Medicine of the University of Liège, the core methodology relies on aggregation of inventor’s pioneering expertises in the fields of stem cells engineering, vitrification of gametes and embryos and in assisted reproductive technologies. VITRICELL will soon provide researchers and clinicians with vitrification kits, allowing upgrade of the current yields and safety after cryopreservation of their high-value cells. The benefits of our method are the most prominent with fragile cell lines limited in expansion ability. VITRICELL’s current product is based on a novel and user-friendly, aseptic and automatable vitrification method in sealed french straws, with bio-safe and chemically defined media. The absence of any ice crystal formation avoids excessive cell dehydration and injuries to cell membranes. As a consequence, VITRICELL’s method results in a higher efficiency (recovery rates, morphology, pluripotency…) than conventional slow freezing for cryopreserving human pluripotent stem cells (hPSCs) and other sensitive stem cell-like lines and embryos from human and non-human species. VITRICELL’s future products developments focuses on (i) vitrification of higher cells numbers in larger containers, (ii) implementation of the method to current automation processes. [less ▲]

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See detailVITRICELL: new efficient method for cryopreserving cells by vitrification
Connan, Delphine ULiege; Ectors, Fabien ULiege; Antoine, Nadine ULiege et al

Conference (2016, September)

Using stem and related cells for clinical purposes requires efficient and bio-safe handling. Cryopreservation is a mandatory key step of storage and transportation, during which cells undergo extreme ... [more ▼]

Using stem and related cells for clinical purposes requires efficient and bio-safe handling. Cryopreservation is a mandatory key step of storage and transportation, during which cells undergo extreme physical and chemical conditions prone to alter their viability as well as their biological properties. Conventional slow-freezing often results in poor survival rates mainly due to excessive cell dehydration and water crystallization. We have addressed this problem by developing a new cryopreservation method based on aseptic and automatable vitrification in sealed french straws. Furthermore, only bio-safe and chemically defined cryopreservation media are used. We have demonstrated that, despite additional constraints, our aseptic vitrification process is more efficient (recovery rates, morphology, pluripotency…) than conventional slow freezing for cryopreserving human pluripotent stem cells (hPSCs). These results have been confirmed on various sensitive stem cell-like lines and embryos from human and non-human species. VITRICELL will soon provide researchers and clinicians with its vitrification kits, allowing to upgrade the current yields and safety after cryopreservation of their high-value cells. [less ▲]

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See detailLong term-cultured and cryopreserved primordial germ cells from various chicken breeds retain high proliferative potential and gonadal colonisation competency
Tonus, Céline ULiege; Cloquette, Karine; Ectors, Fabien ULiege et al

in Reproduction, Fertility and Development (2016), 28(5), 628-639

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See detailEquine cadaver ligaments : A new promising source of stem cells
Shikh Al Sook, Mohamad Khir ULiege; Gabriel, Annick ULiege; Salouci, Moustafa et al

Scientific conference (2015, November 07)

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See detailEctopic Expression of Retrotransposon-Derived PEG11/RTL1 Contributes to the Callipyge Muscular Hypertrophy.
Xu, Xuewen; Ectors, Fabien ULiege; Davis, Erica E. et al

in PLoS ONE (2015), 10(10), 0140594

The callipyge phenotype is an ovine muscular hypertrophy characterized by polar overdominance: only heterozygous +Mat/CLPGPat animals receiving the CLPG mutation from their father express the phenotype ... [more ▼]

The callipyge phenotype is an ovine muscular hypertrophy characterized by polar overdominance: only heterozygous +Mat/CLPGPat animals receiving the CLPG mutation from their father express the phenotype. +Mat/CLPGPat animals are characterized by postnatal, ectopic expression of Delta-like 1 homologue (DLK1) and Paternally expressed gene 11/Retrotransposon-like 1 (PEG11/RTL1) proteins in skeletal muscle. We showed previously in transgenic mice that ectopic expression of DLK1 alone induces a muscular hypertrophy, hence demonstrating a role for DLK1 in determining the callipyge hypertrophy. We herein describe newly generated transgenic mice that ectopically express PEG11 in skeletal muscle, and show that they also exhibit a muscular hypertrophy phenotype. Our data suggest that both DLK1 and PEG11 act together in causing the muscular hypertrophy of callipyge sheep. [less ▲]

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See detailLong term culture, cryopreservation and genetic modification of chicken primordial germ cells
Tonus, Céline ULiege; Garcia Gil, Francisco José ULiege; Cloquette, Karine et al

Poster (2015, October 16)

Avian primordial germ cells (PGCs) are precursor of gametes and appear during early stages of embryonic development. Under appropriate culture conditions, these cells can keep their germ cells properties ... [more ▼]

Avian primordial germ cells (PGCs) are precursor of gametes and appear during early stages of embryonic development. Under appropriate culture conditions, these cells can keep their germ cells properties in vitro and are foreseen as promising tools for developing efficient avian genetic engineering and preservation of germplasm. We propose original methods that allow long term expansion, efficient cryopreservation and genetic modification of primary cultures of undifferentiated PGCs. PGCs are collected from embryonic blood during their migratory period and grown in cell-culture insert in the presence of feeder cells (BRL). This physically separated co-culture system along with selective culture medium promoted emergence, selection and proliferation of PGCs lines. Forty percent of blood samples gave rise to lines originating from three commercial layer and two Belgian endangered breeds. PGCs lines were characterized for the expression of the stem cells and PGCs marker SSEA-1 by FACS. RT-PCR confirmed expression of germ-line specific markers (CVH, CDH, DAZL), pluripotency markers (cPouV, cSox2, cNanog), telomerase and CXCR4 receptor. All lines were male although isolated from pooled male and female blood samples. Two cryopreservation methods were developed based upon slow-freezing and aseptic vitrification. Both have shown a similar effectiveness in allowing storage without phenotype drift. Stably expressing lines were obtained by Lipofectamine® mediated transfection of a GFP plasmid. PGCs were subsequently injected in recipient embryos. Persistence of exogenous PGCs in the developing gonad of recipient embryos confirmed that PGCs retain their gonadal colonisation ability, both after long-term culture and after cryopreservation. [less ▲]

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See detailIntracellular concentration of cryoprotectant during vitrification and slow-freezing cryopreservation procedures
Vanderzwalmen, Pierre ULiege; Ectors, Fabien ULiege; Wirleitner, Barbara et al

in Tucker, Mickael; Lieberman, Juergen (Eds.) Vitrification in assisted reproduction: second edition (2015)

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See detailVitrification of oocytes and embryos: Finally a recognized technique, but still a source of concern and debate
Vanderzwalmen, Pierre ULiege; Zech, Nicolas; Ectors, Fabien ULiege et al

in Tucker, Mickael; Lieberman, Juergen (Eds.) Vitrification in assisted reproduction: second edition (2015)

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See detailPhysiologie humaine
Sherwood, Lauralee; Ectors, Fabien ULiege

Book published by De Boeck Supérieur - 3e édition française (2015)

"Apprendre et comprendre comment fonctionne l'organisme en 750 pages en couleurs, couvrant toutes les grandes fonctions physiologiques" Nouveautés et adaptations de la 3e édition Dans cette 3e édition ... [more ▼]

"Apprendre et comprendre comment fonctionne l'organisme en 750 pages en couleurs, couvrant toutes les grandes fonctions physiologiques" Nouveautés et adaptations de la 3e édition Dans cette 3e édition près de 90% des figures ont été retouchées. La plupart des images des cellules et des figures anatomiques sont nouvelles, elles sont plus réalistes et en perspective. Dans cette édition, des fiches de révision pour chaque chapitre ont été ajoutées à la fin du livre afin que les étudiants puissent réviser les points clés. Des problèmes de réflexion permettent aux étudiants d'analyser des cas cliniques et des symptômes à partir de leurs connaissances. Un contenu riche et pédagogique Tout est mis en oeuvre, dans ce livre, pour aider l'étudiant dans son apprentissage: des contenus riches, détaillés et actuels; de nombreuses aides et précisions à propos de la théorie ainsi qu'une structure claire et logique. De plus, la théorie est ponctuée d'analogies et de références à l'expérience quotidienne. Des schémas et illustrations de qualité Les schémas et illustrations sont proposés dans des couleurs nettes et de qualité. Les couleurs utilisées respectent celles observées dans la réalité pour une meilleure compréhension et intégration des informations. Un guide de révision Cet ouvrage a été pensé de manière à favoriser l'apprentissage de la physiologie et tout particulièrement l'homéostasie. Des notes cliniques viennent ponctuer le discours théorique, des exercices de révision sont disponibles à la fin de chaque chapitre et, à la fin du livre, se trouvent des compélements d'informations au sujet de chaque chapitre, nommés "cartes d'étude". [less ▲]

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