References of "Durenne, Bastien"
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See detailEpoxiconazole exposure affects terpenoid profiles of oilseed rape plantlets based on a targeted metabolomic approach
Durenne, Bastien ULiege; Blondel, Alodie; Druart, Philippe et al

in Environmental Science and Pollution Research (2019)

Epoxiconazole is a broad-spectrum fungicide described as highly persistent in soil and as such can be considered as an abiotic agent like other problematic agrochemicals. Furthermore, the plant ... [more ▼]

Epoxiconazole is a broad-spectrum fungicide described as highly persistent in soil and as such can be considered as an abiotic agent like other problematic agrochemicals. Furthermore, the plant phenotyping tool involving non-invasive monitoring of plant-emitted volatile organic compounds (VOCs) may be useful in the identification of metabolic markers for abiotic stress. We therefore decided to profile the VOCs from secondary metabolism of oilseed rape through a dose-response experiment under several epoxiconazole concentrations (0, 0.01, 0.1 and 1 mg L−1). VOC collections of 35-day-old whole plantlets were performed through a dynamic headspace sampling technique under defined and controlled conditions. The plantlets grew freely within a home-made, laboratory and high-throughput glass chamber without any disturbance. Putative metabolic markers were analysed using a targeted metabolomic approach based on TD-GC-MS method coupled with data acquisition in SIM mode in order to focus on terpenes and sulphur-containing volatiles. Chromatograms of emitted terpenes were achieved accurately for the 35-day-old oilseed rape plantlets. We also analysed the presence of sulphur-containing volatiles in samples of shoot and root tissues using an innovative DHS-TD-GC-MS method, but no difference was found between qualitative profiles. Nevertheless, we demonstrated through this experiment that sesquiterpenes such as β-elemene and (E,E)-α-farnesene are involved in epoxiconazole dose-response. In particular, (E,E)-α-farnesene could serve as a metabolic marker of fungicide exposure for oilseed rape plantlets. [less ▲]

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See detailTargeted metabolomic study in Brassica napus L. under cadmium and epoxiconazole stress
Durenne, Bastien ULiege

Doctoral thesis (2018)

Investigations carried out during this thesis consisted in the research of volatile and non-volatile abiotic stress markers using winter oilseed rape (Brassica napus L.), a major crop worldwide. Two ... [more ▼]

Investigations carried out during this thesis consisted in the research of volatile and non-volatile abiotic stress markers using winter oilseed rape (Brassica napus L.), a major crop worldwide. Two specific abiotic stresses involved in current agricultural soil threats were studied such as cadmium (Cd), a carcinogen trace heavy metal, and epoxiconazole being a persistent systemic fungicide. A targeted metabolomic approach was therefore used through the analysis of volatile organic compounds (VOCs) profiles, targeting terpenoid compounds largely described in plant abiotic stress response, and through the profiling of glucosinolates (GSLs) as non-volatile sulfur-containing metabolites within Brassicaceae. A new glass device allowing the plantlets growth and the non-invasive dynamic sampling of emitted VOCs was successfully developed. It can be described as an innovative laboratory and high-throughput plant chambers system. Oilseed rape plantlets analysis was performed under sterile and controlled conditions, using in vitro medium in the case of experiments related to cadmium exposure and using perlite as a soil-similar substrate for testing epoxiconazole. A phenotyping based on plantlets observations and physiological measurements under cadmium and epoxiconazole stress: i) characteristic symptoms (chloroses), ii) root and shoot growth and iii) biomass was performed at vegetative stage, in complementarity with the targeted metabolomic approach. Different levels of stress were tested on oilseed rape plantlets through dose-response experiments in order to obtain a concentration gradient representing defined stress conditions (i.e. low, middle and severe). With respect to cadmium stress, the concentration of Cd and sulfur (S) has been also measured in the different plant organs such as roots and shoots. GSL profile and content in plantlet organs were also investigated in order to highlight their putative involvement in Cd stress tolerance. About epoxiconazole stress, a characterisation of molecule concentration in the plantlets was carried out before studying its impact on terpenes profiles and on the breakdown products of GSLs (i.e. isothiocyanates) as putative metabolic markers. Overall results showed that metabolic markers could be identified for both kinds of stress such as cadmium and epoxiconazole. These molecules were also involved in adaptive response of plantlets to stress. The sesquiterpenes clearly emerged from the experiments as VOC stress markers. In addition, the role of GSLs in the mechanisms of Cd-tolerance was highlighted with an emphasis on importance of oilseed rape primary S metabolism. The non-invasive method of rapid analysis of VOCs emitted by oilseed rape plantlets and terpenes quantitation could certainly be used for studying the relationships between plant-emitted VOCs and other abiotic or biotic stresses. Finally, this customised glass chambers system could be used in regards to other plants such as potatoes, sugar beets and vegetable crops using soil substrate in order to discover new putative metabolic markers. [less ▲]

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See detailHow cadmium affects the fitness and the glucosinolate content of oilseed rape plantlets
Durenne, Bastien ULiege; Druart, Philippe; Blondel, Alodie et al

in Environmental and Experimental Botany (2018)

Secondary metabolites such as glucosinolates (GSLs) are involved in plant response to biotic stress but can be significantly influenced by abiotic factors as well. Oilseed rape (Brassica napus L ... [more ▼]

Secondary metabolites such as glucosinolates (GSLs) are involved in plant response to biotic stress but can be significantly influenced by abiotic factors as well. Oilseed rape (Brassica napus L.) produces large quantities of several GSLs both in seeds and at the vegetative stage. These sulfur-containing compounds are known to play an important role in cadmium stress tolerance within the Brassicaceae family probably due to specific cross-talk between the S primary and secondary metabolism. Sulfur assimilation is in the middle of multiple metabolic pathways including Cd stress responses at physiological level. Our research focused on the assessment of GSL profiles and content in the roots and shoots of 28-day-old winter oilseed rape plantlets. The study was conducted under in vitro sterile conditions using concentration gradients of 0, 5, 15 and 45 µM of cadmium. A phenotypic analysis was carried out at the end of this experiment in order to evaluate the plantlets’ fitness. Our results described hormetic growth curves for root elongation, root biomass and shoot biomass at Cd concentrations of 5 µM and 15 µM respectively. Our experiment shows that a concentration of 5 µM can be considered as non-toxic, while one of 45 µM represents a lethal dose. Strong relationships were found between Cd accumulated in roots or translocated to shoots and the total sulfur accumulation in the plantlets’ different organs. A decrease of both indole and aliphatic GSL content associated with an increase of Cd accumulation and an increase of total sulfur accumulation was observed in the roots and shoots of the plantlets. It was also further demonstrated that Cd stress has a highly significant effect on roots’ and shoots’ GSL content bringing new insights into GSL’s possible role in the priming of Cd stress. [less ▲]

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See detailPhenotyping of Brassica napus L. plantlets affected during in vitro growth by the presence of epoxiconazole
Durenne, Bastien ULiege; Blondel, Alodie; Ducat, Nathalie et al

in Acta Horticulturae (2018), 1202

Epoxiconazole like others triazole fungicides are known to be persistent in the soil. Several studies using foliar application experiments demonstrated the effect of its triazole metabolite as plant ... [more ▼]

Epoxiconazole like others triazole fungicides are known to be persistent in the soil. Several studies using foliar application experiments demonstrated the effect of its triazole metabolite as plant growth regulator through the anti-gibberellin activity. And notably, the reduction of Brassica napus L. growth can be attributed to the inhibition of gibberellin biosynthesis at the stage of conversion of ent-kaurene to ent-kaurenoic acid. We describe here an in vitro experiment studying the relationship between epoxiconazole in culture medium (0 mg L⁻¹, 0.120 mg L⁻¹ and 0.200 mg L⁻¹) and the phenotyping (root and shoot growth) of three varieties of winter rapeseeds (Brassica napus L. var. Catalina, var. ES Astrid and var. Toccata). Plantlets fungicide content was quantified using the QuEChERS extraction method following by an automated UHPLC-MS/MS analysis. Results showed that the shoots and roots growth of Brassica napus L. plantlets was significantly inhibited by epoxiconazole at 0.120 mg L⁻¹ independently of the variety. The concentration of 0.200 mg L⁻¹ leaded to necrosis and anthocyanosis symptoms and can be considered as lethal for in vitro growing explants. The huge epoxiconazole absorption by rapeseed plantlets clearly showed a dose-dependent relationship and was closely similar for the three varieties. [less ▲]

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See detailA laboratory high-throughput glass chamber using dynamic headspace TDGC/ MS method for the analysis of whole Brassica napus L. plantlet volatiles under cadmium-related abiotic stress
Durenne, Bastien ULiege; Blondel, Alodie; Druart, Philippe et al

in Phytochemical Analysis (2018)

The dynamic headspace sampling technique using thermal desorption, gas chromatography and mass spectrometry (TD-GC/MS) is a powerful method for analysing plant emissions of volatile organic compounds ... [more ▼]

The dynamic headspace sampling technique using thermal desorption, gas chromatography and mass spectrometry (TD-GC/MS) is a powerful method for analysing plant emissions of volatile organic compounds (VOCs). The main purpose of this study was to set-up a laboratory high-throughput glass chamber for whole plant volatiles analysis. The trapping method produced reliable qualitative profiles of oilseed rape VOCs. Moreover, this inexpensive and non-invasive dynamic sampling technique has potential for wide application in sterile approach and replicated research. [less ▲]

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See detailIn vitro monitoring of Artemisia afra using a non-invasive phenotyping system based on VOCs tracking
Durenne, Bastien ULiege; Geerts, Pascal; Fauconnier, Marie-Laure ULiege

Poster (2018)

Secondary metabolites such as volatile organic compounds (VOCs) can be emitted by plants constitutively, with differentiation among and within species, or after de novo synthesis induced by different ... [more ▼]

Secondary metabolites such as volatile organic compounds (VOCs) can be emitted by plants constitutively, with differentiation among and within species, or after de novo synthesis induced by different stresses. VOC emissions could therefore represent an innovative tool as non-invasive marker of abiotic stress and, in particular to monitor phenotypic dynamics.1 We have compared the blend of volatiles emitted by several accessions of 21-day old Artemisia afra plantlets thanks to a laboratory, high-throughput and homemade glass chambers system.2 A. afra is one of the most widely used herbs in traditional medicines in the Asian and African region especially and, has attracted worldwide attention for its possible use in modern diseases in developed countries.The plantlets were propagated in vitro using axillary branching and cultivated under sterile and controlled conditions in a growth chamber equipped with new technology of LED lighting. The VOCs were trapped during 24 hours on Tenax® TA absorbent cartridges with a dynamic headspace sampling method and, were analysed using a thermal desorption unit and using gas chromatography‐mass spectrometry (TD‐GC/MS). Finally, the VOC detection was performed using a quadrupole‐type mass spectrometer (Agilent Technologies). We found very interesting results showing qualitative differences in VOC profiles among the several accessions of A. Afra. Monoterpenoids, sesquiterpenes and artemisia ketone were the major components analysed from chromatograms. In conclusion, this high-throughput and non-invasive system has a wide potential to detect small amounts of emitted VOCs, to discriminate plant genotypes and to monitor plant fitness under both biotic and abiotic stress. Moreover, the volatiles profiling could be also interesting in agricultural research related to other vegetable crops. [less ▲]

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See detailUsing phenotyping and complementary VOC profiling approach to investigate physiological response of Brassica napus L. plantlets under cadmium and epoxiconazole abiotic stresses
Durenne, Bastien ULiege; Geerts, Pascal; Druart, Philippe et al

Poster (2017, November)

Thanks to a laboratory, high-throughput and sterile homemade glass chambers system giving us the capacity to follow VOC (Volatile Organic Compounds) emissions at any time during plant growth, we are able ... [more ▼]

Thanks to a laboratory, high-throughput and sterile homemade glass chambers system giving us the capacity to follow VOC (Volatile Organic Compounds) emissions at any time during plant growth, we are able to compare the phenotype of the plant and its VOC emission in the same time. Although the system could be used for biotic stress too, we decided to investigate the physiological response of oilseed rape plantlets such as model plant concerning two atypical abiotic stresses: cadmium and epoxiconazole. Experiments were achieved under several concentrations corresponding to a gradient from a tolerant to a sublethal dose. Oilseed rape plantlets phenotyping consisted in daily symptoms observation (chlorosis for cadmium stress and shorter internodes length for expoxiconazole stress), shoots and roots length recording and finally, fresh and dry weight assessment. The main goal was therefore to analyse VOC profile with a non-destructive method in parallel to this phenotype characterization in absence of interferences with uncontrolled environmental conditions. Cadmium was chosen because it can be problematic within agricultural soils (phosphate fertilizers among other things) and epoxiconazole because it is a systemic fungicide used in cereals known to be hugely persistent into agricultural soils. [less ▲]

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See detailPlant phenotyping activities at the Walloon Agricultural Research Centre
Vincke, Damien; Durenne, Bastien ULiege; Mingeot, Dominique et al

Poster (2017, November)

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See detailCould indolic glucosinolates root profiling be correlated to Brassica napus L. cadmium stress tolerance?
Durenne, Bastien ULiege; Blondel, Alodie; Druart, Philippe et al

Poster (2017, September)

Glucosinolates profiling among Brassicaceae and Brassica napus L. especially is largely studied regarding biotic stress. Nevertheless literature review could lead to suppose that theses secondary ... [more ▼]

Glucosinolates profiling among Brassicaceae and Brassica napus L. especially is largely studied regarding biotic stress. Nevertheless literature review could lead to suppose that theses secondary metabolites hugely produced within Brassicaceae family can interact in regards to cadmium stress as well. Sulfate primary metabolism plays an important role to Brassica napus L. cadmium stress tolerance and glucosinolates could serve as sulfate pool under such stress conditions. We describe here an in vitro experiment performed under sterile and controlled conditions showing the relationship between Brassica napus L. var. Es Astrid tolerance to a low concentration of cadmium and a decrease of a major indolic glucosinolates produced by the roots of this variety. Phenotyping results obtained from these cadmium stress conditions confirm also that Brassica napus L. remains a highly cadmium tolerant species probably due to glucosinolates root metabolism particularly. [less ▲]

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See detailOral communication: Brassica napus L. plantlets affected during in vitro growth by the presence of epoxiconazole.
Durenne, Bastien ULiege; Blondel, Alodie; Ducat, Nathalie et al

Conference (2017, May 24)

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See detailSolindic: Etude de l'activité biologique du système "Sol-Plante" en vue de préserver les fonctionnalités des sols agricoles
Blondel, Alodie; Durenne, Bastien ULiege; Mingeot, Dominique et al

Report (2016)

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See detailEtude de COVs émis par des lignées de colza in vitro
Durenne, Bastien ULiege

Scientific conference (2016, February)

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See detailUSING TDU-GC-MS TO INVESTIGATE THE VOCS EMISSION OF BRASSICA NAPUS L. PLANTLETS CULTIVATED IN VITRO AND EXPOSED TO CADMIUM ABIOTIC STRESS.
Durenne, Bastien ULiege; Blondel, Alodie; Druart, Philippe et al

in 40th ISCC and 13th GCxGC Symposium Abstracts book (2016)

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See detailA MLPA-oligochromatographic multiplex assay for identification of the toxin genotype and the hypervirulent ribotype O27 of Clostridium difficile
Durenne, Bastien ULiege; Laurent, Thierry; Leclipteux, Thierry et al

in Clinical Microbiology and Infection (2009, May), 15(s4),

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See detailStudy of the usefulness of the data of Bacillus cereus outbreaks in Belgium (2005-2008) for a dose-response modelling purpose
Durenne, Bastien ULiege; Denayer, Sarah; Botteldoorn, Nadine et al

Poster (2009)

Detailed reference viewed: 78 (13 ULiège)