References of "Durenne, Bastien"
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See detailA laboratory high-throughput glass chamber using dynamic headspace TDGC/ MS method for the analysis of whole Brassica napus L. plantlet volatiles under cadmium-related abiotic stress
Durenne, Bastien ULiege; Blondel, Alodie; Druart, Philippe et al

in Phytochemical Analysis (in press)

The dynamic headspace sampling technique using thermal desorption, gas chromatography and mass spectrometry (TD-GC/MS) is a powerful method for analysing plant emissions of volatile organic compounds ... [more ▼]

The dynamic headspace sampling technique using thermal desorption, gas chromatography and mass spectrometry (TD-GC/MS) is a powerful method for analysing plant emissions of volatile organic compounds (VOCs). The main purpose of this study was to set-up a laboratory high-throughput glass chamber for whole plant volatiles analysis. The trapping method produced reliable qualitative profiles of oilseed rape VOCs. Moreover, this inexpensive and non-invasive dynamic sampling technique has potential for wide application in sterile approach and replicated research. [less ▲]

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See detailPhenotyping of Brassica napus L. plantlets affected during in vitro growth by the presence of epoxiconazole
Durenne, Bastien ULiege; Blondel, Alodie; Ducat, Nathalie et al

in Acta Horticulturae (in press)

Epoxiconazole like others triazole fungicides are known to be persistent in the soil. Several studies using foliar application experiments demonstrated the effect of its triazole metabolite as plant ... [more ▼]

Epoxiconazole like others triazole fungicides are known to be persistent in the soil. Several studies using foliar application experiments demonstrated the effect of its triazole metabolite as plant growth regulator through the anti-gibberellin activity. And notably, the reduction of Brassica napus L. growth can be attributed to the inhibition of gibberellin biosynthesis at the stage of conversion of ent-kaurene to ent-kaurenoic acid. We describe here an in vitro experiment studying the relationship between epoxiconazole in culture medium (0 mg L⁻¹, 0.120 mg L⁻¹ and 0.200 mg L⁻¹) and the phenotyping (root and shoot growth) of three varieties of winter rapeseeds (Brassica napus L. var. Catalina, var. ES Astrid and var. Toccata). Plantlets fungicide content was quantified using the QuEChERS extraction method following by an automated UHPLC-MS/MS analysis. Results showed that the shoots and roots growth of Brassica napus L. plantlets was significantly inhibited by epoxiconazole at 0.120 mg L⁻¹ independently of the variety. The concentration of 0.200 mg L⁻¹ leaded to necrosis and anthocyanosis symptoms and can be considered as lethal for in vitro growing explants. The huge epoxiconazole absorption by rapeseed plantlets clearly showed a dose-dependent relationship and was closely similar for the three varieties. [less ▲]

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See detailUsing phenotyping and complementary VOC profiling approach to investigate physiological response of Brassica napus L. plantlets under cadmium and epoxiconazole abiotic stresses
Durenne, Bastien ULiege; Geerts, Pascal; Druart, Philippe et al

Poster (2017, November)

Thanks to a laboratory, high-throughput and sterile homemade glass chambers system giving us the capacity to follow VOC (Volatile Organic Compounds) emissions at any time during plant growth, we are able ... [more ▼]

Thanks to a laboratory, high-throughput and sterile homemade glass chambers system giving us the capacity to follow VOC (Volatile Organic Compounds) emissions at any time during plant growth, we are able to compare the phenotype of the plant and its VOC emission in the same time. Although the system could be used for biotic stress too, we decided to investigate the physiological response of oilseed rape plantlets such as model plant concerning two atypical abiotic stresses: cadmium and epoxiconazole. Experiments were achieved under several concentrations corresponding to a gradient from a tolerant to a sublethal dose. Oilseed rape plantlets phenotyping consisted in daily symptoms observation (chlorosis for cadmium stress and shorter internodes length for expoxiconazole stress), shoots and roots length recording and finally, fresh and dry weight assessment. The main goal was therefore to analyse VOC profile with a non-destructive method in parallel to this phenotype characterization in absence of interferences with uncontrolled environmental conditions. Cadmium was chosen because it can be problematic within agricultural soils (phosphate fertilizers among other things) and epoxiconazole because it is a systemic fungicide used in cereals known to be hugely persistent into agricultural soils. [less ▲]

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See detailPlant phenotyping activities at the Walloon Agricultural Research Centre
Vincke, Damien; Durenne, Bastien ULiege; Mingeot, Dominique et al

Poster (2017, November)

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See detailCould indolic glucosinolates root profiling be correlated to Brassica napus L. cadmium stress tolerance?
Durenne, Bastien ULiege; Blondel, Alodie; Druart, Philippe et al

Poster (2017, September)

Glucosinolates profiling among Brassicaceae and Brassica napus L. especially is largely studied regarding biotic stress. Nevertheless literature review could lead to suppose that theses secondary ... [more ▼]

Glucosinolates profiling among Brassicaceae and Brassica napus L. especially is largely studied regarding biotic stress. Nevertheless literature review could lead to suppose that theses secondary metabolites hugely produced within Brassicaceae family can interact in regards to cadmium stress as well. Sulfate primary metabolism plays an important role to Brassica napus L. cadmium stress tolerance and glucosinolates could serve as sulfate pool under such stress conditions. We describe here an in vitro experiment performed under sterile and controlled conditions showing the relationship between Brassica napus L. var. Es Astrid tolerance to a low concentration of cadmium and a decrease of a major indolic glucosinolates produced by the roots of this variety. Phenotyping results obtained from these cadmium stress conditions confirm also that Brassica napus L. remains a highly cadmium tolerant species probably due to glucosinolates root metabolism particularly. [less ▲]

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See detailOral communication: Brassica napus L. plantlets affected during in vitro growth by the presence of epoxiconazole.
Durenne, Bastien ULiege; Blondel, Alodie; Ducat, Nathalie et al

Conference (2017, May 24)

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See detailSolindic: Etude de l'activité biologique du système "Sol-Plante" en vue de préserver les fonctionnalités des sols agricoles
Blondel, Alodie; Durenne, Bastien ULiege; Mingeot, Dominique et al

Report (2016)

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See detailEtude de COVs émis par des lignées de colza in vitro
Durenne, Bastien ULiege

Scientific conference (2016, February)

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See detailUSING TDU-GC-MS TO INVESTIGATE THE VOCS EMISSION OF BRASSICA NAPUS L. PLANTLETS CULTIVATED IN VITRO AND EXPOSED TO CADMIUM ABIOTIC STRESS.
Durenne, Bastien ULiege; Blondel, Alodie; Druart, Philippe et al

in 40th ISCC and 13th GCxGC Symposium Abstracts book (2016)

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See detailA MLPA-oligochromatographic multiplex assay for identification of the toxin genotype and the hypervirulent ribotype O27 of Clostridium difficile
Durenne, Bastien ULiege; Laurent, Thierry; Leclipteux, Thierry et al

in Clinical Microbiology & Infection (2009, May), 15(s4),

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See detailStudy of the usefulness of the data of Bacillus cereus outbreaks in Belgium (2005-2008) for a dose-response modelling purpose
Durenne, Bastien ULiege; Denayer, Sarah; Botteldoorn, Nadine et al

Poster (2009)

Detailed reference viewed: 71 (10 ULiège)