References of "Defraigne, Jean-Olivier"
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See detailExtracorporeal CO2 removal and regional citrate anticoagulation in an experimental model of hypercapnic acidosis.
Morimont, Philippe ULiege; Habran, Simon; Desaive, Thomas ULiege et al

in Artificial Organs (2019)

Low flow extracorporeal veno-venous CO2 removal (ECCO2 R) therapy is used to remove CO2 while reducing ventilation intensity. However, the use of this technique is limited because efficiency of CO2 ... [more ▼]

Low flow extracorporeal veno-venous CO2 removal (ECCO2 R) therapy is used to remove CO2 while reducing ventilation intensity. However, the use of this technique is limited because efficiency of CO2 removal and potential beneficial effects on pulmonary hemodynamics are not precisely established. Moreover, this technique requires anticoagulation that may induce severe complications in critically ill patients. This study aimed at determining precisely efficiency of CO2 extraction and its effects on right ventricular (RV) afterload, and comparing regional anticoagulation with citrate to systemic heparin anticoagulation during ECCO2 R. ECCO2 R was highly efficient to normalize pH and PaCO2 and to reduce RV afterload resulting from hypercapnic acidosis. Regional anticoagulation with citrate solution was as effective as standard heparin anticoagulation but did not improve CO2 removal and lead to more hypocalcemia and hypotension. [less ▲]

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See detailEx-vivo vasorelaxation induced by polyphenol-rich extracts from plant, fruits and berries on porcine coronary artery rings
Ebabe Elle, Etienne ULiege; Defraigne, Jean-Olivier ULiege; Schini-Kerth, Valérie et al

in Free Radical Biology and Medicine (2018, June), 120

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See detailWhat is the risk of cancer transmission with heart transplantation
Stiennon, Laurie ULiege; TCHANA-SATO, Vincent ULiege; ANCION, Arnaud ULiege et al

Poster (2018, March 16)

The organ donor shortage is particularly severe for heart grafts. Donor history of malignancy is a contra-indication for organ donation, but as the heart is an organ at lower risk of metastasis compared ... [more ▼]

The organ donor shortage is particularly severe for heart grafts. Donor history of malignancy is a contra-indication for organ donation, but as the heart is an organ at lower risk of metastasis compared to the liver or the lungs, the authors aimed to better determine this risk with heart transplantation (HTx) through an extensive review of the literature. Results: 7 cases (melanoma, glioblastoma (2), renal carcinoma, choriocarcinoma, hypernephroma, prostate) of transmission of cancer with HTx were reported. All recipients died from transmitted cancer. Interestingly, in 9 other cases, HTx did not induce cancer transmission at long term, despite the evidence of active cancer in the donor proven by autopsy or by cancer transmission in recipients of other organs from the same donor. Reports of UNOS described 274 heart donors with history of various cancers, without transmission to the recipients. In another report, UNOS reported 212 HTx from donor with brain cancer without cancer transmission to the recipients. Conclusion: HTx is not protected from the risk of donor cancer transmission, but very few cases were reported so far. This fact might indicate that donors with past history of cancer might be considered for heart donation in selected cases, considering the actual organ donor shortage. [less ▲]

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See detailL'ischemie mesenterique aigue.
KERZMANN, Arnaud ULiege; HAUMANN, Alexandre ULiege; BOESMANS, Evelyne ULiege et al

in Revue Medicale de Liege (2018), 73(5-6), 300-303

Acute mesenteric ischemia is a real life-threatening emergency. Mode of presentation is severe abdominal pain with sudden onset. The etiology is most of the time embolus coming from the heart or ... [more ▼]

Acute mesenteric ischemia is a real life-threatening emergency. Mode of presentation is severe abdominal pain with sudden onset. The etiology is most of the time embolus coming from the heart or thrombosis of atherosclerotic vessel wall concerning the superior mesenteric artery. The best investigation is computed tomography with intravenous contrast media injection. Treatment consists of revascularization by open surgery or endovascular approach. Bowel resection may be necessary, as well as delayed second look surgery. This paper aims to review etiologies and therapeutics options for acute mesenteric ischemia. [less ▲]

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See detailWhat we need to know about lipid-associated injury in case of renal ischemia/reperfusion.
ERPICUM, Pauline ULiege; Rowart, Pascal ULiege; Defraigne, Jean-Olivier ULiege et al

in American Journal of Physiology. Renal Physiology (2018)

Renal segmental metabolism is reflected by the complex distribution of the main energy pathways along the nephron, with fatty acid oxidation preferentially used in the cortex area. Ischemia/reperfusion ... [more ▼]

Renal segmental metabolism is reflected by the complex distribution of the main energy pathways along the nephron, with fatty acid oxidation preferentially used in the cortex area. Ischemia/reperfusion injury (IRI) is due to the restriction of renal blood flow, rapidly leading to a metabolic switch towards anaerobic conditions. Subsequent unbalance between energy demand and oxygen/nutrient delivery compromises kidney cell functions, resulting to a complex inflammatory cascade including the production of reactive oxygen species (ROS). Renal IRI especially involves lipid accumulation. Lipid peroxidation is one of the major events of ROS-associated tissue injury. Here, we briefly review the current knowledge of renal cell lipid metabolism in normal and ischemic conditions. Next, we focus on renal lipid-associated injury, with emphasis on its mechanisms and consequences during the course of IRI. Finally, we discuss preclinical observations aiming at preventing and/or attenuating lipid-associated IRI. [less ▲]

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See detailLes urgences cardiaques et vasculaires : du diagnostic au traitement.
Scheen, André ULiege; Lancellotti, Patrizio ULiege; Defraigne, Jean-Olivier ULiege et al

in Revue Médicale de Liège (2018), 73(5-6), 221-223

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See detailThe potency of commercial blackcurrant juices to induce relaxations in porcine coronary artery rings is not correlated to their antioxidant capacity but to their anthocyanin content
Tabart, Jessica ULiege; Auger, Cyril; Kevers, Claire ULiege et al

in Nutrition (2018), 51-52

Objective: Polyphenol-rich products such as fruit juices have been shown to have strong antioxidant capacities and to induce potent endothelium-dependent relaxations. We have evaluated whether the ... [more ▼]

Objective: Polyphenol-rich products such as fruit juices have been shown to have strong antioxidant capacities and to induce potent endothelium-dependent relaxations. We have evaluated whether the commercial blackcurrant juices induced endothelium-dependent relaxation of isolated coronary arteries can be related to their antioxidant capacity and/or phenolic content. Research Methods & Procedures: Six different commercial blackcurrant juices were selected. Their main phenolic compounds were measured by UPLC and antioxidant capacity was evaluated by spectrometric methods. Vascular reactivity studies with these juices were done using isolated porcine coronary arteries. Results: The six different commercial blackcurrant juices induced relaxations ranging from 21 to 100 % at the concentration of 0.5% v/v. The relaxation induced at 0.5 % v/v was not correlated to their antioxidant capacity measured by either ORAC or DPPH assays, and also not to the ascorbic acid, total polyphenols, total flavonols and total phenolic acid contents. In contrast, the amplitude of the relaxation was correlated to the total anthocyanins content and the individual anthocyanin concentration. Conclusions: Correlations between relaxation amplitude and total anthocyanin or individual anthocyanin contents are of interest for the development of functional blackcurrant beverages with the potential to promote vascular protection. [less ▲]

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See detailValidation d’une méthode UHPLC/MS-MS pour le dosage plasmatique du 15-F2t-isoprostane, biomarqueur du stress oxydant, en vue de l’établissement de valeurs de référence en routine clinique
Dubrowski, Thomas ULiege; PINCEMAIL, Joël ULiege; Vigor, Claire et al

Poster (2017, October)

Objectif : Cela fait des décennies que l’implication des isoprostanes dans le stress oxydant est mise en évidence dans la littérature. Malgré cela, il y a peu de concordances entre les différentes valeurs ... [more ▼]

Objectif : Cela fait des décennies que l’implication des isoprostanes dans le stress oxydant est mise en évidence dans la littérature. Malgré cela, il y a peu de concordances entre les différentes valeurs de référence publiées dans ces mêmes articles. Cela peut s’expliquer par le très grand nombre de composés apparentés à cette famille de molécules. L’Unité GLP-AEPT s’est concentrée sur le 15-F2t-isoprostane, biomarqueur « gold standard » de la peroxydation lipidique, afin de développer et valider une méthode de dosage fiable et utilisable en routine clinique. Cette méthode a ensuite été appliquée à l’analyse du plasma prélevé chez 61 volontaires sains en vue de déterminer les valeurs de référence pour ce biomarqueur du stress oxydant. Méthodes : Les échantillons plasmatiques sont purifiés par extraction liquide-solide avant d’être analysés par chromatographie liquide à ultra haute pression couplée à la spectrométrie de masse en tandem (UHPLC/MS-MS) au moyen du système UPLC Xevo TQ-S (Waters). Résultats : La méthode a été validée avec succès sur une gamme de concentrations allant de 25 à 2000 pg/ml en appliquant une stratégie basée sur l’erreur totale de mesure (incluant l’expression de la justesse et de la fidélité intermédiaire) et les profils d’exactitude. Les limites d’acceptation ont été fixées à ± 20 % pour les concentrations inférieures à 30 pg/ml et ± 15 % pour les concentrations égales ou supérieures à cette valeur. Cette approche garantit que seules 10 % des futures mesures d’échantillons inconnus seront en dehors de ces limites. Les concentrations obtenues pour les 61 volontaires sains sont distribuées selon une courbe gaussienne classique. L’intervalle de valeurs de référence pour le 15-F2t-isoprostane a été calculé et est compris entre 152 et 368 pg/ml [1]. Plusieurs corrélations ont ensuite pu être mises en évidence entre ce biomarqueur et d’autres paramètres biologiques connus pour affecter le niveau de stress oxydant de l’organisme (rapport Cu/Zn et glutathion total entre autres) [1]. Conclusion : Une méthode d’analyse fiable et compatible avec une application en routine clinique est à présent disponible afin de mesurer la concentration plasmatique en 15-F2t-isoprostane. Grâce à l’intervalle de valeurs de référence qui a été déterminé pour ce biomarqueur, le niveau de stress oxydant du patient peut être évalué. Références : 1. Pincemail J. et coll. Validated routine-ready UHPLC/MS-MS method for the reference range determination in human plasma of 15-F2t-isoprostane, biomarker of the oxidative stress. FRBM. 2017; 108 (1): page S42. [less ▲]

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See detailIgG4-related disease causing rapid evolution of a severe aortic valvular stenosis
BRULS, Samuel ULiege; Courtois, Audrey ULiege; DELVENNE, Philippe ULiege et al

in Annals of Thoracic Surgery (2017)

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See detailImplications of the calcium-sensing receptor in ischemia/reperfusion.
Paquot, Francois ULiege; Huart, Justine; Defraigne, Jean-Olivier ULiege et al

in Acta Cardiologica (2017), 72(2), 125-131

The calcium-sensing receptor (CaSR) is a G protein-coupled receptor (GPCR) which was first isolated from bovine parathyroid glands. Its complex structure has been well characterized, which helped to ... [more ▼]

The calcium-sensing receptor (CaSR) is a G protein-coupled receptor (GPCR) which was first isolated from bovine parathyroid glands. Its complex structure has been well characterized, which helped to better understand its function. The CaSR activity can be modulated by various ligands, either activators (also called "calcimimetics") or inhibitors (or "calcilytics"). The main role of the CaSR concerns Ca2+ homeostasis. In bone, intestine and kidney, the CaSR acts as a sensor for extracellular ionized Ca2+ concentration ([Ca2+]e) to keep it stable. Such a homeostatic function is well illustrated by human inherited diseases caused by mutations in CASR gene, characterized by Ca2+ balance disturbances. Interestingly, the CaSR is also expressed in numerous tissues which are not directly involved in Ca2+ regulation. There, the CaSR has been implicated in regulatory pathways, including cell proliferation, differentiation and apoptosis. Moreover, recent observations suggest that the CaSR may be involved in ischaemia/reperfusion (I/R) cascades. In cardiomyocytes, the expression and activation of the CaSR are significantly induced at the time of I/R, which induces apoptotic pathways. Likewise, the activation of the CaSR in I/R in brain, liver and kidney has been associated with increased cell death and aggravated structural and functional damage. The present review summarizes these observations and hypothesizes a novel therapeutic option targeting the CaSR in I/R. [less ▲]

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See detailA novel SMAD3 mutation caused multiple aneurysms in a patient without osteoarthritis symptoms
Courtois, Audrey ULiege; Coppieters, Wouter ULiege; BOURS, Vincent ULiege et al

in European Journal of Medical Genetics (2017)

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See detailThoracic endovascular aortic repair (TEVAR) in proximal (type A) aortic dissection: Ready for a broader application?
Nienaber, Christoph A.; Sakalihasan, Natzi ULiege; Clough, Rachel E. et al

in Journal of Thoracic and Cardiovascular Surgery (2017)

OBJECTIVE: Thoracic endovascular aortic repair (TEVAR) has demonstrated encouraging results and is gaining increasing acceptance as a treatment option for aortic aneurysms and dissections. Yet, its role ... [more ▼]

OBJECTIVE: Thoracic endovascular aortic repair (TEVAR) has demonstrated encouraging results and is gaining increasing acceptance as a treatment option for aortic aneurysms and dissections. Yet, its role in managing proximal aortic pathologies is unknown-this is important because in proximal (Stanford type A) aortic dissections, 10% to 30% are not accepted for surgery and 30% to 50% are technically amenable for TEVAR. We describe our case series of type A aortic dissections treated by using TEVAR. METHODS: Between year 2009 and 2016, 12 patients with acute, subacute, or chronic type A aortic dissection with the proximal entry tear located between the coronaries and brachiocephalic artery were treated with TEVAR at 3 centers. Various stent-graft configurations were used to seal the proximal entry tear in the ascending aorta under rapid pacing. RESULTS: A total of 12 patients (9 male, 3 female), mean age 81 +/- 7 years, EuroSCORE II 9.1 +/- 4.5, underwent TEVAR for the treatment of type A aortic dissection. Procedural success was achieved in 11 of 12 patients (91.7%). There was 1 minor stroke and 1 intraprocedural death. No additional deaths were reported at 30 days. At 36 months, there were 4 further deaths (all from nonaortic causes). The mean survival of these 4 deceased was 23 months (range 15-36 months). Follow-up computed tomography demonstrated favorable aortic remodeling. CONCLUSIONS: TEVAR is feasible and reveals promising early results in selected patients with type A aortic dissection who are poor candidates for surgical repair. The current iteration of stent-graft technology, however, needs to be adapted to features specific to the ascending aorta. [less ▲]

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See detailPolyphenol content and antioxidant capacity in various commercial juices
Matute Matute, Alexis Fernando ULiege; Tabart, Jessica; CHERAMY-BIEN, Jean-Paul ULiege et al

Poster (2016, November 16)

Antioxidants are enzymes, non-enzymatic proteins, minerals micronutrients, vitamins, phenolic compounds, pigments and others. Polyphenols have the ability to reduce or prevent damage caused by free ... [more ▼]

Antioxidants are enzymes, non-enzymatic proteins, minerals micronutrients, vitamins, phenolic compounds, pigments and others. Polyphenols have the ability to reduce or prevent damage caused by free radicals promoting positive effects on human health due to their antioxidant capacity. In this study we evaluated the content of polyphenols from several commercial biological (n = 6) and conventional juices (n = 6 ) (orange, blackcurrant, apple, grapes, tomato, carrot, pomegranate, lemon, grapefruit juices) and we determined the antioxidant effect by inhibition of the superoxide anion production measured by chemiluminescence technology. Conventional juices (orange, apple, tomato, pomegranate, lemon, grapefruit juices) showed more total polyphenolics (+ 32%), more flavan-3-ols (+ 67%) and more flavonols (+ 42%) than the same biological juices. The presence of anthocyanins was reported in juices made with blackcurrant, pomegranate and grapes only, with values above 50 µg/mL, regardless of their nature. Surprisingly, chemiluminescence results showed an inhibition in superoxide anion production by 51% for biological juices vs 23% in conventional ones. The correlation between total polyphenol content and antioxidant capacity of all the juices was r = 0.83 (r2 = 0.7). The fact that there is less polyphenols and higher antioxidant activity in bio juices could be related to the concentration and the type of polyphenol present. [less ▲]

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See detailNuclear Magnetic Resonance Metabolomic Profiling of Mouse Kidney, Urine and Serum Following Renal Ischemia/Reperfusion Injury.
Jouret, François ULiege; Leenders, Justine ULiege; Defraigne, Jean-Olivier ULiege et al

in PLoS ONE (2016), 11(9), 1-14

Abstract BACKGROUND: Ischemia/reperfusion (I/R) is the most common cause of acute kidney injury (AKI). Its pathophysiology remains unclear. Metabolomics is dedicated to identify metabolites involved in ... [more ▼]

Abstract BACKGROUND: Ischemia/reperfusion (I/R) is the most common cause of acute kidney injury (AKI). Its pathophysiology remains unclear. Metabolomics is dedicated to identify metabolites involved in (patho)physiological changes of integrated living systems. Here, we performed 1H-Nuclear Magnetic Resonance metabolomics using urine, serum and kidney samples from a mouse model of renal I/R. METHODS: Renal 30-min ischemia was induced in 12-week-old C57BL/6J male mice by bilaterally clamping vascular pedicles, and was followed by 6, 24 or 48-hour reperfusion (n = 12/group). Sham-operated mice were used as controls. Statistical discriminant analyses, i.e. principal component analysis and orthogonal projections to latent structures (OPLS-DA), were performed on urine, serum and kidney lysates at each time-point. Multivariate receiver operating characteristic (ROC) curves were drawn, and sensitivity and specificity were calculated from ROC confusion matrix (with averaged class probabilities across 100 cross-validations). RESULTS: Urine OPLS-DA analysis showed a net separation between I/R and sham groups, with significant variations in levels of taurine, di- and tri-methylamine, creatine and lactate. Such changes were observed as early as 6 hours post reperfusion. Major metabolome modifications occurred at 24h post reperfusion. At this time-point, correlation coefficients between urine spectra and conventional AKI biomarkers, i.e. serum creatinine and urea levels, reached 0.94 and 0.95, respectively. The area under ROC curve at 6h, 24h and 48h post surgery were 0.73, 0.98 and 0.97, respectively. Similar discriminations were found in kidney samples, with changes in levels of lactate, fatty acids, choline and taurine. By contrast, serum OPLS-DA analysis could not discriminate sham-operated from I/R-exposed animals. CONCLUSIONS: Our study demonstrates that renal I/R in mouse causes early and sustained metabolomic changes in urine and kidney composition. The most implicated pathways at 6h and 24h post reperfusion include gluconeogenesis, taurine and hypotaurine metabolism, whereas protein biosynthesis, glycolysis, and galactose and arginine metabolism are key at 48h post reperfusion. [less ▲]

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