References of "Clercx, Cécile"
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See detailClostridium difficile beyond stools: dog nasal discharge as a possible new vector of bacterial transmission
Rodriguez, Cristina; Taminiau, Bernard ULiege; Bouchafa, L. et al

in Heliyon (in press)

Zoonotic transmission of Clostridium difficile has been largely hypothesised to occur after direct or indirect contact with contaminated animal faeces. Recent studies have reported the presence of the ... [more ▼]

Zoonotic transmission of Clostridium difficile has been largely hypothesised to occur after direct or indirect contact with contaminated animal faeces. Recent studies have reported the presence of the bacterium in the natural environment, including in soils and rivers. If C. difficile spores are scattered in the environment, they can easily enter the respiratory tract of dogs, and therefore, dog nasal discharge could be a direct route of transmission not previously investigated. This study reports for the first time the presence of C. difficile in the respiratory tracts of dogs. The bacterium was isolated from 6 (17.1%) out of 35 nasal samples, with a total of 4 positive dogs (19%). C. difficile was recovered from both proximal and distal nasal cavities. All isolates were toxigenic and belonged to PCR-ribotype 014, which is one of the most predominant types in animals and in community-acquired C. difficile infections in recent years. The findings of this study demonstrate that the nasal cavity of dogs is contaminated with toxigenic C. difficile, and therefore, its secretions could be considered as a new route by which bacteria are spread and transmitted. [less ▲]

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See detailA questionnaire-based survey of owner-reported environment and care of West Highland white Terrier with or without idiopathic pulmonary fibrosis
Roels, Elodie ULiege; Fastrès, Aline ULiege; McGeown et al

Poster (2018, October 12)

Canine idiopathic pulmonary fibrosis (CIPF) is a progressive parenchymal lung disease of unknown origin and poorly understood pathophysiology that mainly occurs in old West Highland white terriers (WHWTs ... [more ▼]

Canine idiopathic pulmonary fibrosis (CIPF) is a progressive parenchymal lung disease of unknown origin and poorly understood pathophysiology that mainly occurs in old West Highland white terriers (WHWTs). Not all dogs from the WHWT breed develop CIPF, which suggests the involvement of triggering factors in the onset and/or progression of the disease. To assess potential triggers, an online questionnaire–based survey was submitted to WHWTs owners. The questionnaire was accessible during a 3-month period in 3 different languages (English, French, and Dutch) and was distributed to WHWTs owners and breeders through social medial and emails. Questions were divided into sections regarding owner, dog (clinical and diagnostic data for CIPF), environment (house and surroundings, compost, ventilation, air conditioning, humidification, asbestosis, smoking, and household maintenance), grooming, and veterinary care (vaccination, deworming, and comorbidities). Completed questionnaires were received from 458 WHWTs owners from various countries including principally USA (n=193), Australia (n=62), France (n=56), UK (n=39), and Belgium (n=30). Thirty-eight questionnaires were discarded due to lack of essential data resulting in a total amount of 420 exploitable responses, 138 of which concerning a CIPF affected WHWT. Median age reported at CIPF diagnosis was 11.5 years (range 2– 6.8). Inspiratory crackles were noticed in 82.3% of dogs. Seventy-three out of 138 (52.9%) CIPF WHWTs were dead at the time of questionnaire completion. The overall survival time after diagnosis was 1.4 years (0–8.5). Cause of death was CIPF-related in 76.7% of cases. In order to assess potential triggers associated with CIPF, each CIPF dog was matched to one unaffected WHWT by age. Univariate logistic regression analysis was used for group comparison (CIPF vs. CTRL). Statistical significance was set at a P-value ≤ 0.05. Parameters significantly associated with CIPF included genetic relationship with another dog affected with CIPF (P=0.025), living in an old house (P=0.012), absence of a ventilation system (P<0.0001), and frequent grooming in dedicated facilities (P=0.001). CIPF dogs were at increased risk of cardiac disease (P=0.001), most likely due to the development of secondary pulmonary hypertension, and at decreased risk of endocrine disease (P=0.01) compared with CTRL. The preliminary results of this survey suggest an association between CIPF, genetic background and environmental factors such as old nonventilated houses or grooming habits. Further analysis of the results of the questionnaire are ongoing [less ▲]

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See detailEffect of antimicrobial drug on lung microbiota in healthy dogs.
Fastrès, Aline ULiege; Vangrinsven, Emilie ULiege; Tutunaru, Alexandru-Cosmin ULiege et al

in Journal of Veterinary Internal Medicine (2018, October 12)

In human and dogs, baseline differences in lung microbiota (LM) have been associated with important clinical features in chronic lung diseases (CLD) and there is growing evidence that an altered LM ... [more ▼]

In human and dogs, baseline differences in lung microbiota (LM) have been associated with important clinical features in chronic lung diseases (CLD) and there is growing evidence that an altered LM contributes to disease pathogenesis. The common use of antibiotic drugs throughout the management of CLD likely represents a major confounding factor in the study of the LM. However, the effect of antibiotic treatment on the LM in healthy individuals has not been specifically investigated yet. The aim of the present study was to assess the short and medium-term effect of an oral treatment with a large spectrum antimicrobial drug on the LM in healthy dogs. Six healthy experimental beagle dogs were included. Amoxycillin/clavulanic acid (AC) was administered at a dose of 20 mg/kg twice daily for 10 days. In each dog, bronchoalveolar lavage fluid (BALF) was collected at 3 different timepoints: before administration of AC (J0) and immediately (J10) as well as 16 days (J26) after interruption of AC. In each BALF, total and differentiated cell counts were obtained and metagenetic analyses were performed on the V1-V3 hypervariable region of 16S rDNA after total bacterial DNA extraction and sequencing on a MiSeq Illumina sequencer. Taxonomical assignation and microbiota community analysis were done with MOTHUR V1.35 with an OTU clustering distance of 0.03. Statistical comparisons between events for microbiota community and BALF cell counts were made using Friedman test and post-hoc t-tests with Bonferroni correction. Statistical differences in bacterial population relative abundance between timepoints were assessed using a mixed linear model with FDR correction for multiple comparisons. Administration of AC did not induce significant changes in BALF cellular counts and had no effect on the richness, evenness and alpha diversity. Bacteroidetes and Proteobacteria abundance increased from J0 to J10 (mean: 12.0 to 24.1, and 12.3 to 26.2%, respectively; p<0.001) and decreased at J26 (13.1 and 9.3%; p<0.001). Firmicutes abundance decreased from J0 to J10 (45.6 to 18.3%; p<0.001) and increased at J26 (28.8%; p<0.001). Actinobacteria abundance increased at J26, compared with J0 and J10 (43.7 compared with 23.2 and 23.5% respectively; p<0.001). We failed to show significant differences between timepoints at the level of genera and species. In conclusion, in healthy dog, oral administration of a commonly used large spectrum antimicrobial drug induces significant changes in microbial population abundance at the phyla level. Most but not all of these changes normalize within 2 weeks after discontinuation of the drug. [less ▲]

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See detailAnalysis of the lung microbiota in healthy dogs and in canine idiopathic pulmonary fibrosis, a possible spontaneous model for human IPF.
Fastrès, Aline ULiege; Roels, Elodie ULiege; Taminiau, Bernard ULiege et al

Poster (2018, September 16)

Canine idiopathic pulmonary fibrosis (CIPF) mimics human IPF and affects old dogs from one breed: the West Highland white terrier (WHWT). In human IPF, lung microbiota is suspected to be associated with ... [more ▼]

Canine idiopathic pulmonary fibrosis (CIPF) mimics human IPF and affects old dogs from one breed: the West Highland white terrier (WHWT). In human IPF, lung microbiota is suspected to be associated with disease pathogenesis and might serve as a therapeutic target. The aim of the study was to characterize microbial alteration associated with breed, environment and CIPF disease. Bronchoalveolar lavage fluid was sampled from 4 groups of dogs: client-owned WHWTs affected with CIPF from Belgium-BE (n=7, 11.6y), healthy client-owned BE-WHWTs (n=5, 11.2y), healthy client-owned WHWTs from Finland-FI (n=5, 11.0y) and healthy experimental BE-beagle dogs (n=6, 8.8y). Metagenetic analysis was performed on V1-V3 hypervariable region of 16S rDNA after total bacterial DNA extraction and sequencing on a MiSeq Illumina sequencer. Data were compared between healthy BE-WHWTs and BE-beagles, healthy BE-WHWTs and FI-WHWTs, and healthy BE-WHWTs and CIPF BE-WHWTs. Data analyses demonstrated that the same phyla (Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes) predominated in all groups. Significant differences (p<005) were mainly noted for species with relative abundance of less than 1%, except for Micrococcus luteus, higher in FI-WHWTs (7.4%) compared to BE-WHWTs (0%) and for Pseudomonas JF766687 and EU373411, Serratia and Hydrogenophilus spp, higher in BE-beagles (15.3, 8.1, 5.0 and 1.5%) compared with BE-WHWTs (0.3, 0.3, 0, 0.1%, respectively). Results indicate that further analysis of the impact of breed or environment and inclusion of larger amounts of dogs are needed before investigation of lung microbiota as a biomarker or therapeutic target in CIPF. [less ▲]

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See detailInvestigation of serum Krebs Von den Lungen 6 concentration as a predisposing factor and in the diagnosis of canine idiopathic pulmonary fibrosis in the West Highland white terrier.
Fastrès, Aline ULiege; Roels, Elodie ULiege; Bolen, Géraldine ULiege et al

Poster (2018, September 06)

Canine idiopathic pulmonary fibrosis (CIPF) mimics human idiopathic pulmonary fibrosis (IPF) and affects old dogs from the West Highland white terrier (WHWT) breed. Due to difficulties to both diagnose ... [more ▼]

Canine idiopathic pulmonary fibrosis (CIPF) mimics human idiopathic pulmonary fibrosis (IPF) and affects old dogs from the West Highland white terrier (WHWT) breed. Due to difficulties to both diagnose early and follow the course of pulmonary fibrosis, numerous biomarkers have been studied in both species. In humans, the mucin Krebs Von den Lungen 6 (KL-6) has consistently been found to be increased in the serum from patients with interstitial lung disease, including IPF and is possibly involved in the IPF pathophysiological process. In CIPF, it has not yet been assessed. In this study, we assessed the diagnostic value of serum KL-6 concentration in CIPF as well as the association between serum KL-6 concentrations and breed predisposition for the disease. Blood was obtained from 5 groups of dogs: WHWTs affected with CIPF (n=20, 14 females (F)/6 males (M), mean age=11.0y, range [5.2-14.5]), healthy aged-matched WHWTs (n=18, 11F/7M, 10.5y [5.3-15.3]), and healthy dogs from other breeds not predisposed for CIPF: Jack Russel terriers (JR) (n=13, 8F/5M, 6.0y [3.0-9.1]), Malinois Belgian shepherds (MBS) (n=13, 5F/8M, 6.5y [2.8-10.8]) and King Charles spaniels (KCS) (n=13, 9F/4M, 6.5y [3.6-9.3]). Serum was stored at -80°C until batched analysis. KL-6 concentration was measured with a commercially available ELISA test (Canine Krebs Von den Lungen 6 Elisa kit, amsbio). Results were compared using a covariance analysis including the 5 groups defined above, gender and an interaction between groups and gender together with the effect of the age. The analysis was followed by post-hoc comparisons between groups using t-tests. Results show that only the effect of groups was significant (p=0.010). Serum KL-6 concentration was higher in healthy WHWTs (4.8+/-4.0 ng/ml) compared with KCS (1.6+/-1.8 ng/ml) and MBS (2.2+/-1.0 ng/ml) (p=0.001 and 0.013 respectively) and higher in JR (4.1+/-2.9 ng/ml) compared with KCS (p=0.016). There was no difference between CIPF (3.2+/-1.9 ng/ml) and healthy WHWTs (p=0.072). In conclusion, differences in serum KL-6 concentrations were found amongst breeds in healthy dogs, with higher concentrations observed in terrier breeds. Whether this may reflect a predisposing factor for CIPF development merits further investigations. Unlike what has been found in human IPF, KL-6 is not a good serum biomarker for diagnosis as there was no difference between CIPF and unaffected WHWTs. [less ▲]

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See detailComparison of two minimally invasive enilconazole perendoscopic infusion protocols for the treatment of canine sinonasal aspergillosis.
Vangrinsven, Emilie ULiege; Girod, Maud; Goossens, Damien et al

in Journal of Small Animal Practice (2018)

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See detailLa souris, le patient, et le faux expert. Décryptage d'une mystification.
Bakker, Julie ULiege; Balthazart, Jacques ULiege; Baron, Frédéric ULiege et al

Article for general public (2018)

La recherche sur animaux est actuellement encadrée de façon stricte en Wallonie comme dans toute l'Union Européenne (voir l'article de Marc Vandenheede publié dans le Vif). Cette législation et les ... [more ▼]

La recherche sur animaux est actuellement encadrée de façon stricte en Wallonie comme dans toute l'Union Européenne (voir l'article de Marc Vandenheede publié dans le Vif). Cette législation et les contrôles qui y sont associés induisent de nombreuses contraintes pratiques, des charges administratives et des coûts financiers importants que les chercheurs seraient certainement heureux d'éviter s'il existait une alternative à l'expérimentation animale. [less ▲]

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See detailThe lung microbiome in idiopathic pulmonary fibrosis: A promising approach for targeted therapies
Fastrès, Aline ULiege; Felice, Florence ULiege; Roels, Elodie ULiege et al

in International Journal of Molecular Sciences (2017), 18(12), 2735

This review focuses on the role of the lung microbiome in idiopathic pulmonary fibrosis. Although historically considered sterile, bacterial communities have now been well documented in lungs both in ... [more ▼]

This review focuses on the role of the lung microbiome in idiopathic pulmonary fibrosis. Although historically considered sterile, bacterial communities have now been well documented in lungs both in healthy and pathological conditions. Studies in idiopathic pulmonary fibrosis (IPF) suggest that increased bacterial burden and/or abundance of potentially pathogenic bacteria may drive disease progression, acute exacerbations, and mortality. More recent work has highlighted the interaction between the lung microbiome and the innate immune system in IPF, strengthening the argument for the role of both host and environment interaction in disease pathogenesis. Existing published data suggesting that the lung microbiome may represent a therapeutic target, via antibiotic administration, immunization against pathogenic organisms, or treatment directed at gastroesophageal reflux. Taken altogether, published literature suggests that the lung microbiome might serve in the future as a prognostic biomarker, a therapeutic target, and/or provide an explanation for disease pathogenesis in IPF. [less ▲]

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See detailPossible effect of environment on lung microbiota in the healthy West Highland white terrier.
Fastrès, Aline ULiege; Roels, Elodie ULiege; Taminiau, Bernard ULiege et al

Diverse speeche and writing (2017)

Over the past few years, the number of publications about lung microbiota has considerably increased due to the advancement of new molecular technologies. In dogs, only two studies in healthy experimental ... [more ▼]

Over the past few years, the number of publications about lung microbiota has considerably increased due to the advancement of new molecular technologies. In dogs, only two studies in healthy experimental beagles have been published. Interestingly, we recently reported major differences in the lung microbiota composition and diversity between experimental healthy beagles and healthy client-owned West Highland white terriers (WHWT) suggesting an impact of the breed or of the living environment on lung microbiota composition. Accordingly, to further assess the effect of living environment, the aim of the present study was to compare lung microbiota data obtained in healthy WHWT (n = 5) living in Finland with those previously obtained in healthy WHWT (n = 5) living in Belgium. For this purpose, bronchoalveolar lavage was retrospectively selected. Metagenetic analysis were performed after DNA extraction and sequencing on a MiSeq Illumina sequencer. Taxonomical assignation and microbiota community analysis were done with MOTHUR V1.35 with an OTU clustering distance of 0.03. Data analyses demonstrated that the same 4 major phyla Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes predominated in both groups of dogs. Significant but subtle differences were observed between groups. Results of the present study do not demonstrate a clear impact of the living environment on lung microbiota composition. In conclusion, analysis of larger cohorts of healthy dogs with various living conditions appears necessary. [less ▲]

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See detailComparison between sedation and general anesthesia for high resolution computed tomographic characterization of canine idiopathic pulmonary fibrosis in west highland white terriers
Roels, Elodie ULiege; Couvreur, Thierry; Farnir, Frédéric ULiege et al

in Veterinary Radiology and Ultrasound (2017)

Canine idiopathic pulmonary fibrosis (CIPF) is a progressive interstitial lung disease mainly affecting West Highland white terriers (WHWTs). Thoracic high-resolution computed tomographic (T-HRCT ... [more ▼]

Canine idiopathic pulmonary fibrosis (CIPF) is a progressive interstitial lung disease mainly affecting West Highland white terriers (WHWTs). Thoracic high-resolution computed tomographic (T-HRCT) findings for CIPF acquired under general anesthesia have been described previously. However, the use of general anesthesia may be contraindicated for some affected dogs. Sedation may allow improved speed and safety, but it is unknown whether sedation would yield similar results in identification and grading of CIPF lesions. The aim of this prospective, observational, method-comparison, case-control study was to compare findings from T-HRCT images acquired under sedation versus general anesthesia for WHWTs affected with CIPF (n=11) and age-matched controls (n=9), using the glossary of terms of the Fleischner Society and a scoring system. Ground-glass opacity (GGO) was identified in all affected WHWTs for both sedation and general anesthesia acquisitions, although the GGO extent varied significantly between the two acquisitions (P<0.001). Ground-glass opacity was the sole lesion observed in control dogs (n=6), but was less extensive compared with affected WHWTs. Identification and grading of a mosaic attenuation pattern differed significantly between acquisitions (P<0.001). Identification of lesions such as consolidations, nodules, parenchymal and subpleural bands, bronchial wall thickening, and bronchiectasis did not differ between acquisitions. The present study demonstrated that T-HRCT obtained under sedation may provide different information than T-HRCT obtained under general anesthesia for identification and grading of some CIPF lesions, but not all of them. These differences should be taken into consideration when general anesthesia is contraindicated and sedation is necessary for evaluating WHWTs with CIPF. [less ▲]

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See detailDiagnostic Value of PV/PA ratio in dogs with PH of pre-capillary origin
Roels, Elodie ULiege; Merveille, Anne-Christine ULiege; Malaize, Pierre-Louis et al

in Journal of Veterinary Internal Medicine (2016, September)

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See detailInvestigation of the coagulation system in canine idiopathic pulmonary fibrosis
Roels, Elodie ULiege; Bauer, N.; Lecut, Ch. et al

Poster (2016, September)

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See detailComparative analysis of the respiratory microbiota of healthy dogs and dogs with canine idiopathic pulmonary fibrosis
Roels, Elodie ULiege; Taminiau, Bernard ULiege; Darnis, Elodie ULiege et al

in Journal of Veterinary Internal Medicine (2016, September)

Detailed reference viewed: 33 (4 ULiège)