References of "Chichiricco, Pauline Marie"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailIn situ photochemical crosslinking of hydrogel membrane for guided tissue regeneration
Chichiricco, Pauline Marie ULiege; Riva, Raphaël ULiege; Thomassin, Jean-Michel ULiege et al

in Dental Materials (2018), 34(12), 1769-1782

Periodontitis is an inflammatory disease that destroys the tooth-supporting attachment apparatus. Guided tissue regeneration (GTR) is a technique based on a bar- rier membrane designed to prevent wound ... [more ▼]

Periodontitis is an inflammatory disease that destroys the tooth-supporting attachment apparatus. Guided tissue regeneration (GTR) is a technique based on a bar- rier membrane designed to prevent wound space colonization by gingival cells. This study examined a new formulation composed of two polymers that could be photochemically cross-linked in situ into an interpenetrated polymer network (IPN) forming a hydrogel mem- brane. Methods. We synthetized and characterized silanized hydroxypropyl methylcellulose (Si- HPMC) for its cell barrier properties and methacrylated carboxymethyl chitosan (MA-CMCS) for its degradable backbone to use in IPN. Hydrogel membranes were cross-linked using riboflavin photoinitiator and a dentistry visible light lamp. The biomaterial’s physicochem- ical and mechanical properties were determined. Hydrogel membrane degradation was evaluated in lysozyme. Cytocompatibility was estimated by neutral red uptake. The cell bar- rier property was studied culturing human primary gingival fibroblasts or human gingival explants on membrane and analyzed with confocal microscopy and histological staining. Results. The IPN hydrogel membrane was obtained after 120 s of irradiation. The IPN showed a synergistic increase in Young moduli compared with the single networks. The CMCS addition in IPN allows a progressive weight loss compared to each polymer network. Cyto- compatibility was confirmed by neutral red assay. Human cell invasion was prevented by hydrogel membranes and histological sections revealed that the biomaterial exhibited a barrier effect in contact with soft gingival tissue. [less ▲]

Detailed reference viewed: 38 (7 ULiège)
See detailPhoto-crosslinkable hydrogel for guided periodontal tissue regeneration
Chichiricco, Pauline Marie ULiege; Riva, Raphaël ULiege; Thomassin, Jean-Michel ULiege et al

Conference (2018, May 29)

Periodontitis is an inflammatory disease which destroys the tooth-supporting attachment apparatus. Guided Tissue Regeneration (GTR) is a technique based on a barrier membrane designed to prevent the wound ... [more ▼]

Periodontitis is an inflammatory disease which destroys the tooth-supporting attachment apparatus. Guided Tissue Regeneration (GTR) is a technique based on a barrier membrane designed to prevent the wound space colonization by gingival cells. Indeed, these cells, characterized by a faster migration and proliferation rate compared to bone and periodontal ligament cells, could interfere with the regeneration process. In this work, we developed a liquid formulation composed of a mixture of silanized hydroxypropylmethyl cellulose (Si-HPMC) and methacrylated carboxymethyl chitosan (MA-CMCS) able to be photochemically crosslinked in situ leading to hydrogel membrane made of an interpenetrated polymer network (IPN) with a synergistic combination of favorable properties of each polymer network. Si-HPMC was selected to assess the barrier effect against soft tissue gingival invasion and CMCS to control the membrane degradation due to its degradable backbone. The methacrylate grafted chains on CMCS allows a photo-curing in short time using visible light lamp (λ 420-480 nm) already used in dentistry and when vitamin B2 (or riboflavin) is used as photoinitiator. The gel point of the IPN precursor formulation was assessed by rheological measurement under lamp irradiation. Mechanical properties after photocrosslinking were studied. Hydrogel membrane degradation was evaluated by soaking hydrogels in 10 mg/ml lysozyme buffer at 37 °C, which confirmed that the addition of CMCS modified the degradation profile of Si-HPMC hydrogel, as evidenced by the recorded weight loss. The in vitro cytocompatibility was evaluated by neutral red uptake using murine fibroblast (L929) in accordance to ISO 10993-5 specifications. Finally, we studied the barrier effect by culturing human primary gingival fibroblasts or human gingival explants on top of the membrane. Cell infiltration was analyzed with confocal microscopy and histological staining (hematoxylin, eosin Y and safranin (HES)). In the next future, in vivo experiment will be performed to further characterize the material for periodontal regeneration. [less ▲]

Detailed reference viewed: 16 (2 ULiège)
See detailSelf-setting hydrogel for 3D bioprinting
Chichiricco, Pauline Marie ULiege; Rosa, B.; De Villemagne, P. et al

Conference (2017, July 05)

Detailed reference viewed: 35 (6 ULiège)
See detailInterpenetrated polymer network hydrogel for biomedical applications
Chichiricco, Pauline Marie ULiege; Rethore, Gildas; Boyer, Cecyle et al

Poster (2017, June 28)

Detailed reference viewed: 42 (7 ULiège)
See detailPhoto-crosslinkable hydrogel for guided periodontal tissue regeneration
Chichiricco, Pauline Marie ULiege; Riva, Raphaël ULiege; Thomassin, Jean-Michel ULiege et al

Poster (2017, May 04)

Periodontitis is an inflammatory disease resulting from the presence of oral bacteria biofilm in periodontal tissue, which destroys the tooth-supporting attachment apparatus. Untreated inflammation can ... [more ▼]

Periodontitis is an inflammatory disease resulting from the presence of oral bacteria biofilm in periodontal tissue, which destroys the tooth-supporting attachment apparatus. Untreated inflammation can spread to the gum tissue and lead, ultimately, to the loosening of the supporting tooth bone, with the risk that the tooth eventually falls. Guided Tissue Regeneration is a technique based on the application of a barrier membrane designed to prevent colonization of the wound space by epithelial cells from soft tissues. Indeed, these cells, characterized by a faster migration and proliferation rate compared to bone and periodontal ligament cells, could interfere with the regeneration process. In previously work Struillou et al. demonstrated the benefit effect of silated hydroxypropylmethylcellulose (Si HMPC)-based hydrogel can act as an efficient physical barrier in periodontal defect. Typically, this material is able to form a 3D network through the condensation of silanoate groups at physiological pH. However, a decrease of gelation time is necessary to assure the stability in peripheral part of the wound. In this project, we developed an injectable photo-crosslinkable membrane based on methacrylated carboxymethyl chitosan (CMCs) and Si HPMC that can be applied as a viscous solution and cured in situ in presence of a photoinitiator system made of riboflavin and triethanolamine. A visible light lamp (λ 420-480 nm), already used in dentistry, was preferred over a UV lamp. The addition of methacrylated polymer increase the stability of the material and increase the mass loss, in order to improve the bioresorption of the membrane. The chemical grafting of methacrylated carboxymethyl chitosan was characterized by 1H NMR and Infrared Spectroscopy. The gel point of the solution was determined by rheology and remained compatible with a clinical application. Moreover, the biocompatibility of this biomaterials was tested using murine cells using two assay: Neutral Red assay and MTT Cell Proliferation Assay. The in vitro tests validate the chemical synthesis in a biological point of view. The irradiation on cells and the direct contact with hydrogel doesn’t have an impact on cells viability. The capability of this material to act as a physical barrier was also evaluated using human gingival fibroblast. The cells were isolated from human gum explant before being put in contact with the hydrogel. After four days of contact no cells invasion was observed in the hydrogel using confocal microscopy. These preliminary results are quite promising for the development of novel injectable systems for Guided Periodontal Regeneration. In the future work, in vivo assays will be performed in Periodontal defect in a canine model. [less ▲]

Detailed reference viewed: 97 (3 ULiège)
See detailPhotocrosslinked hydrogel for periodontal tissue regeneration
Chichiricco, Pauline Marie ULiege; Riva, Raphaël ULiege; Thomassin, Jean-Michel ULiege et al

Conference (2016, December 15)

Detailed reference viewed: 21 (4 ULiège)
See detailPhotocrosslinked hydrogels for guided periodontal tissue regeneration
Chichiricco, Pauline Marie ULiege; Weis, Pierre; Struillou, Xavier et al

Conference (2015, October)

Detailed reference viewed: 38 (4 ULiège)