References of "CAVALIER, Etienne"
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See detailVitamin D nutritional status and bone turnover markers in childhood acute lymphoblastic leukemia survivors: A PETALE study
Delvin, E.; Alos, N.; Rauch, F. et al

in Clinical Nutrition (in press)

Background: The remarkable progress in the treatment of childhood acute lymphoblastic leukemia (cALL) has led to a survival rate reaching 90%. This success story is unfortunately linked to increased risk ... [more ▼]

Background: The remarkable progress in the treatment of childhood acute lymphoblastic leukemia (cALL) has led to a survival rate reaching 90%. This success story is unfortunately linked to increased risk of impaired skeletal mass accumulation during childhood and adolescence, predisposing the patients to osteoporosis and pathological fractures at adulthood. Objective: This study aims at characterizing the vitamin D status and bone health biomarkers in a well-characterized cohort of cALL survivors. Results: Food frequency questionnaires reveal that (i) the total vitamin D intake varies greatly (44–2132 IU/d), (ii) only 16.8% of the participants consume vitamin D supplements, and (iii) 74% of survivors' intakes are below the Recommended Daily Intakes (400 IU/d). For the 42 participants taking vitamin D supplements, the median (2.5–97.5%iles) intake is 600 IU/d (21.2–1972 IU/d). Sixteen participants are vitamin D deficient (<30 nM) and 66 insufficient (≥30 – <50 nM). Serum 24,25(OH)2D3 concentrations are directly related to those of 25OHD3, and those of 3-epi-25OHD3 below the Lower Limit of Quantification in most samples. The participants' serum concentrations of cross-linked C-telopeptide of type-I collagen and intact amino-terminal pro-peptide of type-I collagen decrease steadily with age, leveling at adulthood, and are at all times higher in males. Conclusion: The present study shows that the prevalence of vitamin D insufficiency or deficiency is not greater in cALL survivors compared to the general Canadian population despite low vitamin D food and supplement intakes. Furthermore, there seem to be no overt imbalance in the gender- and age-adjusted serum bone turnover marker concentrations. © 2018 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism [less ▲]

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See detailDiagnostic accuracy of three automated urine analyzers compared to urine culture
Castiglione, Vincent ULiege; GADISSEUR, Romy ULiege; Bernard, Maxime ULiege et al

Poster (2018, November 16)

Urine culture is an important diagnosis method of urinary tract infection. However, it is time-consuming and results aren’t available quickly. Automated urine analyzers have been developed to screen urine ... [more ▼]

Urine culture is an important diagnosis method of urinary tract infection. However, it is time-consuming and results aren’t available quickly. Automated urine analyzers have been developed to screen urine samples more rapidly. The goal of this study was to compare three automated analyzers to urine culture: the Atellica UAS 800 (Siemens, Munich, Germany), the UF-4000 (Sysmex, Kobe, Japan) and the SediMAX (Menarini, Florence, Italy). We first validated each analyzer. We analyzed then 318 samples with the three analyzers within 2 hours after sample reception. An urine aliquot was collected before sediment analysis for bacteria culture. Ten microliters of un-centrifuged urine was inoculated on blood agar and CLED agar plates, then they were incubated aerobically at 36°C for 24 h. Bacteria count of each analyzer was compared to urine culture to determine diagnostic accuracy. The result was considered positive when the bacteria growth reached 104 CFU/ml. We also used the results of leukocytes and nitrites results from the strip measurement to improve the accuracy. The abilities of the UF-4000 to distinguish Gram positive (GP) from Gram negative (GN) bacteria, and of the UAS 800 to identify rod and cocci, were determined. [less ▲]

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See detailUrine sediment analysis: comparison of three automated analyzers to manual microscopy
Castiglione, Vincent ULiege; GADISSEUR, Romy ULiege; Bernard, Maxime ULiege et al

Poster (2018, November 16)

Urine microscopic analysis is an old method that reveals information about kidney health. Several automated analyzers, that are less time-consuming, are currently available, but manual microscopy is still ... [more ▼]

Urine microscopic analysis is an old method that reveals information about kidney health. Several automated analyzers, that are less time-consuming, are currently available, but manual microscopy is still the gold-standard method. The goal of this study was to validate and compare three automated analyzers to manual microscopy: the Atellica UAS800 (Siemens, Munich, Germany), the UF-4000 (Sysmex, Kobe, Japan) and the SediMax (Menarini, Florence, Italy). We first validated each analyzer. A total of 359 samples were analyzed with the three analyzers and with a manual microscope within 2 hours. Two trained reviewers used a microscope with bright field, contrast phase and polarized light to identify urine elements. The diagnostic accuracy was determined thanks to microscopy results. [less ▲]

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See detailComparison of three automated strip analyzers to Cobas 800 for the analysis of glucose, proteins, albumin and creatinine
Castiglione, Vincent ULiege; GADISSEUR, Romy ULiege; Bernard, Maxime ULiege et al

Poster (2018, November 16)

Urine glucose, proteins, albumin and creatinine are measured for the screening of diabetes and renal diseases. The automated strip analyzers are used for quick screening of large populations. The goal of ... [more ▼]

Urine glucose, proteins, albumin and creatinine are measured for the screening of diabetes and renal diseases. The automated strip analyzers are used for quick screening of large populations. The goal of this study was to compare a more accurate method, the Cobas 8000 (Roche, Bale, Switzerland) to three automated analyzers: the Clinitek Novus (Siemens, Munich, Germany), the UC-3500 (Sysmex, Kobe, Japan) and the AutionMax (Menarini, Florence, Italy). A total of 284 urine samples were prospectively collected for the comparison. The samples were analyzed on the three analyzers within 2 hours. Before the analysis of samples by each method, an aliquot was frozen at -80°C. All samples were then defrost and analyzed in one batch with the Cobas 8000 (Roche, Bale, Switzerland) within the month. The diagnostic accuracy was determined thanks to the results of the Cobas 8000. However, the creatinine and albumin cannot be assessed with the AutionMax. [less ▲]

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See detailThematic Approaches: the exemple of Bone metabolism
Cavalier, Etienne ULiege

Conference (2018, November 11)

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See detailClinical data on rare Sulfamethoxazole crystalluria assessed by Fourier Transform Infrared Spectrophotometry
CASTIGLIONE, Vincent ULiege; CAVALIER, Etienne ULiege; GADISSEUR, Romy ULiege

in Data in Brief (2018)

The data contained in this article are related to the article entitled “Case report: Uncommon Sulfamethoxazole Crystalluria” [1]. Sulfamethoxazole crystalluria is very rare and crystals identification is ... [more ▼]

The data contained in this article are related to the article entitled “Case report: Uncommon Sulfamethoxazole Crystalluria” [1]. Sulfamethoxazole crystalluria is very rare and crystals identification is complex [2], [3]. We identified seven patients with uncommon urine crystals that were composed of N-Acetyl-Sulfamethoxazole. Three of the patients developed an acute renal failure simultaneously to crystalluria. Hence, this article describes the method of crystals identification thanks to infrared spectroscopy. The relevant clinical data of patients, including medical history, drug dosage and urine parameters related to the crystalluria are presented. [less ▲]

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See detailMatrix-Gla-Protein: un nouveau marqueur de lithiase urinaire?
Castiglione, Vincent ULiege; DELANAYE, Pierre ULiege; LUKAS, Pierre ULiege et al

Conference (2018, October 19)

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See detailCESI-MS Workflow for Protein Quantification
Nyssen, Laurent ULiege; Fillet, Marianne ULiege; CAVALIER, Etienne ULiege et al

Poster (2018, October)

Introduction: Sheathless CE-MS interfaces allow increase in sensitivity by coupling low-flow electrospray ionization and tandem MS detection. Peak intensity will depend on spray voltage as well as ... [more ▼]

Introduction: Sheathless CE-MS interfaces allow increase in sensitivity by coupling low-flow electrospray ionization and tandem MS detection. Peak intensity will depend on spray voltage as well as migration and injection conditions. Nevertheless, these parameters influence each other and require methodical optimization to get the most of each instrument. In the present work we share our experience with sheathless CE-MS and neutral coating to analyze peptide and protein samples. Methods: Experiments were conducted on a CESI 8000 capillary electrophoresis holding a neutral coated OptiMS cartridge and coupled to a QT 6500 mass spectrometer. Separation buffer and voltage, curtain gas and source temperature were conserved through experiments. Separation pressure and source voltage were optimized while applying voltage and pressure on separation buffer spiked with the peptide used (pI 9.5 marker from the Advance cIEF starter kit by Sciex). A daily reference run was used to compare modifications to the injection despite variable capillary performance. Finally, shifts in spray voltage due to injection parameters were determined using sequences of runs with different spray voltages. Preliminary results: Decreasing separation pressure from 5 to 1.5 psi increased peptide intensity; electrokinetic injection (EKI) increased peak intensity compared to hydrodynamic injection (HDI); the HDI of a water plug before the EKI increased peak intensity further, as well as a high percentage of acetonitrile in the sample medium. Finally, we compared our initial and our final conditions. In both cases, a positive Q1 scan of 1000 Da/s for m/z 300 to 1000 was acquired, and the electropherograms display the extracted ion current for a 1 m/z interval centered on the m/z of the doubly charged peptide. In the initial method, the peptide was diluted in BGE and was introduced by HDI (1 % of total length); 5 psi pressure were applied to both inlet and outlet; source voltage was 1800 V. When analyzing a 1:160 (v/v) dilution of the peptide, the intensity recorded for [M+2H]2+ was 9.3e7 counts. In the final method, the peptide was diluted in 75:25 acetonitrile:water (v/v) and was introduced by EKI (+ 10 kV 100s). Before the EKI, a HDI of water (0.5 % of total length) was performed, and after the EKI, separation buffer was introduced by HDI (0.5 % of total length). The separation pressure was changed to 1.5 psi and the spray voltage adjusted to 1600 V. When analyzing a 1:160000 (v/v) dilution of the peptide, the recorded intensity of [M+2H]2+ was 9e8 counts. Therefore, following these guidelines, we were able to increase intensity by a 10000 factor. Novel aspect: Frequent monitoring of spray voltage and peak intensity in similar conditions allows good inter-run comparison and troubleshooting. [less ▲]

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See detailValidation of 24 blood gas analyzers, GEM 5000 Premier
GADISSEUR, Romy ULiege; MUSSO, Giuseppe ULiege; Cavalier, Etienne ULiege et al

Poster (2018, September 28)

Introduction: Point-of-care blood gas test results may help to take therapeutic decision by their immediate impact on patient care. Recently, a novel cartridge-type blood gas analyzer, the GEM Premier ... [more ▼]

Introduction: Point-of-care blood gas test results may help to take therapeutic decision by their immediate impact on patient care. Recently, a novel cartridge-type blood gas analyzer, the GEM Premier 5000 (IL-Werfen) was commercialized and 24 analyzers were installed at the University Hospital of Liège. One of them was installed in the main central laboratory, the 23 other ones were installed into 19 patient care units. Before the implementation, we evaluated the analytical performance of all the 24 GEM Premier 5000, for the determination of whole blood pH, pCO2 and pO2, electrolytes (Na+, K+, Cl−), ionized calcium (iCa2+), glucose, lactate and co-oximetry parameters ((total hemoglobin (tHb), oxyhemoglobin (O2Hb), carboxyhemoglobin (COHb), methemoglobin (metHb), deoxyhemoglobin (HHb)). Method: First, we evaluated the performance of the GEM Premier 5000 dedicated to the central lab, so-called “referent analyzer”, with 3 levels of External Quality Controls material (EQC RNA Medicals). CLSI EP5 recommends 2 replicates per run, 1 or 2 runs per day, for a minimum of 20 days. Nevertheless, we analyzed the 3 levels of EQC, 2 replicates per run, 2 runs per day during 5 consecutive days. Afterwards, on the whole 24 GEM Premier 5000, we analyzed aqueous QC material (Werfen GEM System Evaluator, level 1-2), 3 replicates within a single run, once per day, during 5 consecutive days. We determined the manufacturer's claim for Within-Run and Total precisions for each. Co-oxymetry parameters were not evaluated on 4 analyzers. Then, we compared the all 23 analyzers to the “referent analyzer” of the central lab. Therefore, for each parameter, we showed in a Youden diagram all the results obtained by 23 analyzers. The position of the acceptance ranges were shown graphically using the specifications for Acceptable(%) Root Mean Standard Deviation (RMSD) proposed by the German Guidelines for Quality (RILIBAK) for whole blood parameter, for different ranges of parameters. Results: The results showed a good correlation between analyzers excepted for some parameters. Lactate and MetHb: level 2 were often over-estimated when compared to “Reference Analyzer”. It could be explained by the fact that this IQC level contains very low Lactate and MetHb rates. The pO2 level2: some results were over estimated (random errors >< cassette reagents >< low values). The pO2 level1: over-estimated with 8 analyzers letting us think that the cassette reagent of our “Reference Analyzer” had a bias in the lower range. Conclusion: Performance evaluation of a large cluster of Blood Gas Analyzers is always a challenge for a Hospital. Accreditation is one of the main goal in each Belgian laboratory. Hospital Accreditation is also discussed in Belgium. This study shows an interesting approach to validate Blood Gas Analyzers for highlighting data. Based on our study results, we estimated that the evaluated instrument are a suitable blood gas analyzer for both POCT and laboratory use. [less ▲]

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See detailCortisol everywhere!
Arias-Carnero, Benjamin; PEETERS, Stéphanie ULiege; DEVILLE, Marine ULiege et al

Poster (2018, September 12)

Our problem with a routine LC-MS/MS system is related to the presence of an isobaric interference of cortisol from an unknown source. After cleaning and checking the proper function of the LC system, we ... [more ▼]

Our problem with a routine LC-MS/MS system is related to the presence of an isobaric interference of cortisol from an unknown source. After cleaning and checking the proper function of the LC system, we performed an exhaustive study of all the possible contamination sources that can affect the method performance. Bovine Serum Albumin (BSA) seems the critical reactive that contained the highest amount of this interference, among other sources tested. Identity of this compound was obtained by QTOF analysis and resulted to be cortisol. [less ▲]

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See detailDevelopment and Validation of the Simultaneous Measurement of Estrone and 17β-Estradiol in Serum by LC-MS/MS for Clinical Laboratory Applications
Pitarch-Motellón, Jorge; Cavalier, Etienne ULiege; LE GOFF, Caroline ULiege et al

Poster (2018, September 12)

A straightforward analytical method for the determination of estrone (E1) and 17β-estradiol (E2) at ultra-low levels in serum samples by LC-MS/MS has been developed and validated. This method entails an ... [more ▼]

A straightforward analytical method for the determination of estrone (E1) and 17β-estradiol (E2) at ultra-low levels in serum samples by LC-MS/MS has been developed and validated. This method entails an extraction and derivatization with dansyl chloride followed by separation with a C18 column. A comparison of the developed LC-MS/MS method against our routine immunoassay shows a good correlation for E2 while an important negative bias is observed for E1 by RIA. The later comparison for E1 shows the need to switch from the current routine automated immunoassays to highly-sensitive LC-MS/MS quantifications in order to provide accurate and reliable clinical results, especially at very low levels. [less ▲]

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See detailRaman Chemical Imaging, a new tool in kidney stone structure analysis: Case-study and comparison to Fourier Transform Infrared spectroscopy
Castiglione, Vincent ULiege; Sacre, Pierre-Yves ULiege; CAVALIER, Etienne ULiege et al

in PLoS ONE (2018), 13(8), 0201460

Background and objectives: The kidney stone’s structure might provide clinical information in addition to the stone composition. The Raman chemical imaging is a technology used for the production of two ... [more ▼]

Background and objectives: The kidney stone’s structure might provide clinical information in addition to the stone composition. The Raman chemical imaging is a technology used for the production of two-dimension maps of the constituents' distribution in samples. We aimed at determining the use of Raman chemical imaging in urinary stone analysis. Material and methods: Fourteen calculi were analyzed by Raman chemical imaging using a confocal Raman microspectrophotometer. They were selected according to their heterogeneous composition and morphology. Raman chemical imaging was performed on the whole section of stones. Once acquired, the data were baseline corrected and analyzed by MCR-ALS. Results were then compared to the spectra obtained by Fourier Transform Infrared spectroscopy. Results: Raman chemical imaging succeeded in identifying almost all the chemical components of each sample, including monohydrate and dihydrate calcium oxalate, anhydrous and dihydrate uric acid, apatite, struvite, brushite, and rare chemicals like whitlockite, ammonium urate and drugs. However, proteins couldn't be detected because of the huge autofluorescence background and the small concentration of these poor Raman scatterers. Carbapatite and calcium oxalate were correctly detected even when they represented less than 5 percent of the whole stones. Moreover, Raman chemical imaging provided the distribution of components within the stones: nuclei were accurately identified, as well as thin layers of other components. Conversion of dihydrate to monohydrate calcium oxalate was correctly observed in the centre of one sample. The calcium oxalate monohydrate had different Raman spectra according to its localization. Conclusion: Raman chemical imaging showed a good accuracy in comparison with infrared spectroscopy in identifying components of kidney stones. This analysis was also useful in determining the organization of components within stones, which help locating constituents in low quantity, such as nuclei. However, this analysis is time-consuming, making it more suitable for research studies rather than routine analysis. [less ▲]

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See detailCARDIAC BIOMARKERS FLUCTUATION IN RUNNERS OF MARATHONS, SEMI-MARATHONS AND UNTRAINED RUNNERS
Le Goff, Caroline ULiege; VRANKEN, Laura ULiege; Kaux, Jean-François ULiege et al

in 23rd Annual Congress of the ECSS (2018, July)

INTRODUCTION: Regular exercise like running is one important part of the prevention program of cardiovascular disease. There are several studies on biomarker changes during marathons especially cardiac ... [more ▼]

INTRODUCTION: Regular exercise like running is one important part of the prevention program of cardiovascular disease. There are several studies on biomarker changes during marathons especially cardiac biomarkers have been studied and mild to moderate elevations have been described as a results of a running exercise Exact underlying mechanism for these biomarker elevations reflecting physiological or even pathobiological changes is unknown and less trained athletes might exhibit a higher risk compared to well trained. The aim of our study was to compare three cardiac biomarkers for ischemic condition , cardiac stretch and fibrotic processes were tested in different type of runners, trained marathon and semi-marathon runners and untrained runners before, directly after and 3 hours after the running exercise. METHODS: 23 marathon runners, 15 semi-marathon runners <44.1±8.4yo> and 17 healthy sedentary subjects < 37± 4.4 yo> were enrolled in our study. Blood samples were taken just before , just after and 3 hours after the race, centrifuged, aliquoted and stored frozen at -80°C before further analysis. The study was approved by the Ethical Committee of our University Hospital. The analyses were performed on the Abbott ARCHITECT i2000SR for the hs cTnI, BNP and Gal-3 and on the C8000 for hs-cTnT and NTproBNP according to the manufacturer’s instructions for use. RESULTS: In all 3 running groups there is an increase of cardiac biomarkers Troponin I, BNP, Galectin-3 and NT-ProBNP after completion of the physical exercise. Biomarkers increase is depending on the intensity and duration of the exercise and is higher in long distance marathon and semi-marathon runners compared to the control group with a 1 hour run. Cardiac biomarker levels between trained marathon and semi-marathon runners were not statistically different in the pre-exercise baseline samples for BNP, NT-Pro-BNP and Galectin-3. Compared to untrained runners only Troponin I levels were higher in baseline sample of marathon runners when compared to controls, cardiac Troponin T was less significant. CONCLUSION: In conclusion, the question whether running exercise of different intensity could be harmful to the heart has no simple answer. We could show that running exercise can be associated with biochemical abnormalities that may reflect adverse consequences on the heart like possible micro necrosis, oxidative stress, fibrosis and myocardial stretch. With exception of Troponin where levels continue to raise after end of running, NPs and Gal-3 levels normalized relatively fast after the exercise. The possible harmful effect of longer term cardiac consequences of repeated intensive sport acitvities still needs to be demonstrated. [less ▲]

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See detailMeasured (and estimated) glomerular filtration rate: reference values in West Africa.
Yayo, Eric; Aye, Mireille; Yao, Carine et al

in Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association (2018), 33(7), 1176-1180

Background: Establishment of normal reference values for glomerular filtration rate (GFR) is mandatory in nephrology. However, no data are available for measured GFR (mGFR) in Africa. Methods: GFR was ... [more ▼]

Background: Establishment of normal reference values for glomerular filtration rate (GFR) is mandatory in nephrology. However, no data are available for measured GFR (mGFR) in Africa. Methods: GFR was measured in 237 healthy adult subjects (103 women and 134 men, mean age 34 +/- 10 years) by iohexol plasma clearance. Results: The mean mGFR was 103 +/- 17 mL/min/1.73 m 2 and the median value was 103 mL/min/1.73 m 2 (2.5th and 97.5th percentiles are 76 and 137 mL/min/1.73 m 2 , respectively). No significant difference in mGFR results was observed in patients < 40 years of age, whereas a significant decline in mGFR was observed after 40 years of age. There was no significant difference between mGFR in men and women. Conclusions: Normal GFR values and descriptions of percentiles are now available for West Africa. As in Caucasians, no significant difference was observed between men and women. Moreover, the same age-associated decline in mGFR is also observed after 40 years of age, as in Caucasians. [less ▲]

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See detailA Randomized Study to Compare a Monthly to a Daily Administration of Vitamin D(3) Supplementation.
De Niet, Sophie; Coffiner, Monte; Da Silva, Stephanie et al

in Nutrients (2018), 10(6),

We aimed to determine whether a cumulative dose of vitamin D(3) produces the same effects on the serum concentration of 25(OH)D(3) if it is given daily or monthly. This is a monocentric, two-armed ... [more ▼]

We aimed to determine whether a cumulative dose of vitamin D(3) produces the same effects on the serum concentration of 25(OH)D(3) if it is given daily or monthly. This is a monocentric, two-armed, randomized, interventional, open, and parallel study conducted from November 2016 to March 2017 in Belgium. We randomized 60 subjects with vitamin D deficiency to receive 2000 IU vitamin D(3) daily or 50,000 IU monthly. The same cumulative dose of vitamin D(3) was given to each treatment group (150,000 IU). The 25(OH)D(3) serum concentrations from baseline to day 75 were 14.3 +/- 3.7 to 27.8 +/- 3.9 ng/mL in the monthly group and 14.1 +/- 3.4 to 28.8 +/- 5.4 ng/mL in the daily group. The mean change versus the baseline level was significantly different between the groups at day 2, 4, 7, and 14 and no longer different from day 25. One day after the intake of vitamin D(3), as expected, serum 25(OH)D(3) and 1,25(OH)(2)D(3) increased significantly in the monthly group, whereas they did not change significantly in the daily group. The median time to reach the 20 ng/mL target concentration was significantly different in the two groups, in favor of the monthly regimen (1 day versus 14 days; p = 0.02). In conclusion, a monthly administration of 50,000 IU vitamin D(3) provides an effective tool for a rapid normalization of 25(OH)D(3) in deficient subjects. A daily administration of the same cumulative dose is similarly effective but takes two weeks longer to reach the desirable level of 20 ng/mL. [less ▲]

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