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See detailUnraveling the role of transient starch in the response of Arabidopsis to elevated CO 2 under long-day conditions
Jauregui, Ivan ULiege; Pozueta-Romero, Javier; Córdoba Jacoste, Francisco Javier ULiege et al

in Environmental and Experimental Botany (2018), 155

Previous studies on Arabidopsis under long-term exposure to elevated CO2 have been conducted using starch synthesis and breakdown mutants cultured under short day conditions. These studies showed that ... [more ▼]

Previous studies on Arabidopsis under long-term exposure to elevated CO2 have been conducted using starch synthesis and breakdown mutants cultured under short day conditions. These studies showed that starch synthesis can ameliorate the photosynthetic reduction caused by soluble sugar-mediated feedback regulation. In this work we characterized the effect of long-term exposure to elevated CO2 (800 ppm) on growth, photosynthesis and content of primary photosynthates in long-day grown wild type plants as well as the near starch-less (aps1) and the starch-excess (gwd) mutants. Notably, elevated CO2 promoted growth of both wild type and aps1 plants but had no effect on gwd plants. Growth promotion by elevated CO2 was accompanied by an increased net photosynthesis in WT and aps1 plants. However, the plants with the highest starch content (wild type at elevated CO2, gwd at ambient CO2, and gwd at elevated CO2) were the ones that suffered decreased in in vivo maximum carboxylation rate of Rubisco, and therefore, photosynthetic down-regulation. Further, the photosynthetic rates of wild type at elevated CO2 and gwd at elevated CO2 were acclimated to elevated CO2. Notably, elevated CO2 promoted the accumulation of stress-responsive and senescence-associated amino acid markers in gwd plants. The results presented in this work provide evidence that under long-day conditions, temporary storage of overflow photosynthate as starch negatively affect Rubisco performance. These data are consistent with earlier hypothesis that photosynthetic acclimation can be caused by accelerated senescence and hindrance of CO2 diffusion to the stroma due to accumulation of large starch granules. [less ▲]

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See detailAcclimation to elevated CO2 is improved by low Rubisco and carbohydrate content, and enhanced Rubisco transcripts in the G132 barley mutant
Córdoba Jacoste, Francisco Javier ULiege

in I Spanish Symposium on Cereal Physiology and Breeding (2018)

Variations induced by mutagenesis may identify targets for adapting to rising atmospheric CO2concentrations. G132 is a barley (Hordeum vulgare L.) mutant with strong decreases in photosynthesis,Rubisco ... [more ▼]

Variations induced by mutagenesis may identify targets for adapting to rising atmospheric CO2concentrations. G132 is a barley (Hordeum vulgare L.) mutant with strong decreases in photosynthesis,Rubisco and carbohydrate content, but increased Rubisco transcripts. We tested the hypothesis that G132will record a greater stimulation of photosynthesis and dry mass gain than the wild-type (WT) in responseto CO2 enrichment. A growth chamber experiment compared the mutant G132 and the Graphic WTdeveloped in ambient (390 μmol mol-1) and elevated (1200 μmol mol-1) CO2. Photosynthesis, protein andcarbohydrate contents, plant dry matter and leaf area were determined. The transcriptome responses toelevated CO2 were investigated using microarrays and qRT-PCR. CO2 enrichment increased leaf area inG132 but not in WT, and induced greater dry mass increases in the mutant, attributable to increases in totalgreen area in the former. Growth in elevated CO2 stimulated photosynthesis more in G132 than in WT, inassociation with a lack of Rubisco down-regulation and lower carbohydrate levels in G132. Elevated CO2induced more changes in gene expression in WT than G132. Clustering of the corresponding transcriptsshowed that the expression of genes involved in carbohydrate synthesis, in G132, cell wall synthesis andmodification, in WT, nitrate reductase, significantly more in G132 than WT, and jasmonate metabolism inG132 was enhanced, while that for stress-related genes was repressed in both genotypes by elevated CO2.Rising atmospheric CO2 decreased the transcript levels for a greater number of transcription factors in WTthan in G132. The low Rubisco and carbohydrate content and enhanced Rubisco transcripts recorded inG132 are traits that may improve adaptation to elevated CO2. [less ▲]

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See detailAnalysisi of Euglena gracilis proteome under mixotrophic conditions
Córdoba Jacoste, Francisco Javier ULiege; Perez, Emilie; Baurain, Denis ULiege et al

Conference (2017)

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See detailAnalysis of Euglena gracilis proteome under mixotrophic conditions
Córdoba Jacoste, Francisco Javier ULiege; Perez, Emilie; Baurain, Denis ULiege et al

Scientific conference (2017)

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See detailAcclimation to elevated CO2 is improved by low Rubisco and carbohydrate content, and enhanced Rubisco transcripts in the G132 barley mutant
Córdoba Jacoste, Francisco Javier ULiege; Pérez, P.; Morcuende, R. et al

in Environmental and Experimental Botany (2017), 137

Variations induced by mutagenesis may identify targets for adapting to rising atmospheric CO2 concentrations. G132 is a barley mutant with strong decreases in photosynthesis, Rubisco and carbohydrate ... [more ▼]

Variations induced by mutagenesis may identify targets for adapting to rising atmospheric CO2 concentrations. G132 is a barley mutant with strong decreases in photosynthesis, Rubisco and carbohydrate content, but increased Rubisco transcripts. We tested the hypothesis that G132 will record a greater stimulation of photosynthesis and dry mass gain than the wild-type (WT) in response to CO2 enrichment. A growth chamber experiment compared the mutant G132 and the WT developed in ambient (∼390 μmol mol−1) and elevated (1200 μmol mol−1) CO2. Photosynthesis, protein and carbohydrate contents, Rubisco and nitrate reductase activities, leaf morphology, plant dry matter and leaf area were determined. The transcriptome responses of fully expanded leaves of G132 and WT to elevated CO2 were investigated using microarrays and qRT-PCR. Growth in elevated CO2 stimulated photosynthesis more in G132 than in WT, in association with a lack of Rubisco down-regulation and lower carbohydrate levels in G132. Electron transport-related proteins increased in G132 and decreased in WT. Elevated CO2 induced more changes in gene expression in WT than G132. Clustering of the corresponding transcripts showed that the expression of genes involved in carbohydrate synthesis and nitrogen assimilation was enhanced, while that for stress-related genes was repressed in both genotypes, and gene expression for jasmonate metabolism was activated in G132. Elevated CO2 decreased the transcript levels for a greater number of transcription factors in WT than in G132. CO2 enrichment increased leaf area in G132 but not in WT, and induced greater dry mass increases in the mutant. The low Rubisco and carbohydrate content and enhanced Rubisco transcripts recorded in G132 are traits that may improve adaptation to rising CO2. © 2017 Elsevier B.V. [less ▲]

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See detailCharacterization of the G132 mutant barley with potential adaptation to climate change
Valeros, Fatima; Martinez-Carrasco, Rafael; Morcuende, Rosa et al

in New Biotechnology (2016), 33(3), 427

The barley breeding programmes seek for varieties with better adaptation to climate change. The aim of this study was to characterize the G132 mutant, selected in earlier studies on barley adaptation to ... [more ▼]

The barley breeding programmes seek for varieties with better adaptation to climate change. The aim of this study was to characterize the G132 mutant, selected in earlier studies on barley adaptation to high CO2 concentration environments, as compared to the Graphic wild type throughout development. At early growth stages, photosynthesis measured with ambient and saturating atmospheric CO2 was lower in G132 than in Graphic, but recovered later in development. The results show that genotypic differences in photosynthesis were not due to limitations in CO2 diffusion into leaves caused by stomatal closure. It was concluded that the low photosynthesis rate in G132 was due to a decrease in content of ribulose-1,5-bisphosphate carboxylase–oxygenase (Rubisco), which catalyzes the CO2 fixation reaction. The low enzyme content was partly due to enhanced degradation. [less ▲]

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See detailFunctional and transcriptional characterization of a barley mutant with impaired photosynthesis
Córdoba Jacoste, Francisco Javier ULiege; Molina-Cano, J.-L.; Martínez-Carrasco, R. et al

in Plant Science (2016), 244

Chemical mutagenesis induces variations that may assist in the identification of targets for adaptation to growth under atmospheric CO2 enrichment. The aim of this work was to characterize the limitations ... [more ▼]

Chemical mutagenesis induces variations that may assist in the identification of targets for adaptation to growth under atmospheric CO2 enrichment. The aim of this work was to characterize the limitations causing reduced photosynthetic capacity in G132 mutagenized barley (Hordeum vulgare L. cv. Graphic) grown in a glasshouse. Compared to the wild type (WT) G132 showed increased transcript levels for the PSII light harvesting complex, but lower levels of chlorophyll, transcripts for protochlorophyllide oxidoreductase A and psbQ, and PSII quantum efficiency in young leaves. Rubisco limitation had an overriding influence on G132 photosynthesis, and was due to strong and selective decreases in Rubisco protein and activity. These reductions were accompanied by enhanced Rubisco transcripts, but increased levels of a Rubisco degradation product. G132 showed lower levels of carbohydrates, amino acids and corresponding transcripts, and proteins, but not of nitrate. Many of the measured parameters recovered in the mutant as development progressed, or decreased less than in the WT, indicating that senescence was delayed. G132 had a longer growth period than the WT and similar final plant dry matter. The reduced resource investment in Rubisco of G132 may prove useful for studies on barley adaptation to elevated CO2 and climate change. © 2015 Elsevier Ireland Ltd. [less ▲]

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See detailPhotosynthesis-dependent/independent control of stomatal responses to CO<inf>2</inf> in mutant barley with surplus electron transport capacity and reduced SLAH3 anion channel transcript
Córdoba Jacoste, Francisco Javier ULiege; Molina-Cano, J.-L.; Pérez, P. et al

in Plant Science (2015), 239

The mechanisms of stomatal sensitivity to CO<inf>2</inf> are yet to be fully understood. The role of photosynthetic and non-photosynthetic factors in stomatal responses to CO<inf>2</inf> was investigated ... [more ▼]

The mechanisms of stomatal sensitivity to CO<inf>2</inf> are yet to be fully understood. The role of photosynthetic and non-photosynthetic factors in stomatal responses to CO<inf>2</inf> was investigated in wild-type barley (Hordeum vulgare var. Graphic) and in a mutant (G132) with decreased photochemical and Rubisco capacities. The CO<inf>2</inf> and DCMU responses of stomatal conductance (g<inf>s</inf>), gas exchange, chlorophyll fluorescence and levels of ATP, with a putative transcript for stomatal opening were analysed. G132 had greater g<inf>s</inf> than the wild-type, despite lower photosynthesis rates and higher intercellular CO<inf>2</inf> concentrations (C<inf>i</inf>). The mutant had Rubisco-limited photosynthesis at very high CO<inf>2</inf> levels, and higher ATP contents than the wild-type. Stomatal sensitivity to CO<inf>2</inf> under red light was lower in G132 than in the wild-type, both in photosynthesizing and DCMU-inhibited leaves. Under constant C<inf>i</inf> and red light, stomatal sensitivity to DCMU inhibition was higher in G132. The levels of a SLAH3-like slow anion channel transcript, involved in stomatal closure, decreased sharply in G132. The results suggest that stomatal responses to CO<inf>2</inf> depend partly on the balance of photosynthetic electron transport to carbon assimilation capacities, but are partially regulated by the CO<inf>2</inf> signalling network. High g<inf>s</inf> can improve the adaptation to climate change in well-watered conditions. © 2015 Elsevier Ireland Ltd. [less ▲]

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See detailMutagenesis of barley to cope with rising atmospheric CO2
Córdoba Jacoste, Francisco Javier ULiege; Perez, P.; Morcuende, R. et al

Poster (2014)

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See detailPhysiological features of a barley mutant with improved response to high CO2
Córdoba Jacoste, Francisco Javier ULiege; Perez, P.; Morcuende, R. et al

in Vieira, N.; Saibo, N.; Oliveira, M. (Eds.) XIII Congresso Luso-Espanhol de Fisiologia Vegetal. Abstract Book (2013)

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