References of "Benchimol, Lionel"
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See detailInfluence of storage temperature for respiratory epithelial samples on ciliary functional analysis
Benchimol, Lionel ULiege; Bricmont, Noémie ULiege; BOBOLI, Hedwige ULiege et al

Poster (2021, March 06)

Introduction and Aim: Primary ciliary dyskinesia is characterized by inherited stationary or dyskinetic respiratory cilia. Digital high speed videomicroscopy is highly sensitive and specific for diagnosis ... [more ▼]

Introduction and Aim: Primary ciliary dyskinesia is characterized by inherited stationary or dyskinetic respiratory cilia. Digital high speed videomicroscopy is highly sensitive and specific for diagnosis but lacks standardization. Particularly, the temperature for samples conservation has never been studied. The study goal was to compare ciliary analysis after samples storage at 4°C or 22°C. Agreement between two independent observers was sought as secondary goal. Material and Methods: Ciliated epithelial samples were obtained by inferior turbinate brushing from 13 healthy subjects, divided equally and conserved at 4°C or at 22°C. Digital high speed videomicroscopy assessed ciliary beat frequency (CBF) and percentage of normal ciliary beat pattern (CBP) nine hours after sampling. Results: There was no significant difference when samples were stored at 4°C or 22°C. Median CBF was 13.37 (12,23 –15,37) Hz and 15.24 (12,89 –16,06) Hz at 4°C and 22°C respectively (p = 0.210). Percentage of normal CBP was 81,80% (63,75 – 90,65) and 80,00% (71,70 – 87,30) at 4°C and 22°C respectively (p = 0.910). Moreover, inter observer analysis showed high agreement rate with 93,4% for the CBF and 90.0% for the CBP. Conclusion: This pilot study suggested with a high inter observer agreement rate that the temperature for respiratory ciliated samples conservation had no effect on ciliary function analysis. Larger studies are needed to confirm these preliminary results, notably in pathological conditions. [less ▲]

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See detailENT Service during the COVID-19 pandemic: a cross-sectional study in 572 patients.
Peigneux, Nicolas ULiege; Benchimol, Lionel ULiege; BENDAVID, Guillaume ULiege et al

in B-ENT (2021), 16(4),

Objective: To provide objective data on the impact of COVID-19 crisis on our ENT practice. Methods: 572 consecutive patients presenting to the ENT clinic and ENT operating theatre from March 16, 2020 to ... [more ▼]

Objective: To provide objective data on the impact of COVID-19 crisis on our ENT practice. Methods: 572 consecutive patients presenting to the ENT clinic and ENT operating theatre from March 16, 2020 to May 3, 2020 were prospectively included. Demographic and clinical data, admission time, paraclinic testing, management, outcome and follow’up were recorded. Retrospective search for comparison to past year over the same period of time was carried out. Results: Coronavirus crisis and the strict lockdown rules led to a severe disruption of the ENT service. A drop in overall activity of 91.1% was observed ; compared to the 6,454 patients who had been treated in 2019 over the same period, appointments and procedures were restricted to 572 patients in this study. Mortality rates increased from 0.82% to 4.55%. While some patients in real need may not have been able to be seen, others presented without ground. More than a quarter of admissions (n=157, 27.6%) resulted in no specific ENT treatment. Patient selection improved when patients had a recent ENT history (OR=2.39 [1.50-3.81], p=0.0003) or were referred by a physician (OR=5.30 [3.69-7.61], p<0.0001). Conclusions: Our data suggested impaired healthcare of all ENT patients. Special attention should be paid to non-Covid otolaryngology patients. [less ▲]

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See detailNasal Brushing Sampling and Processing using Digital High Speed Ciliary Videomicroscopy - Adaptation for the COVID-19 Pandemic.
Bricmont, Noémie ULiege; Benchimol, Lionel ULiege; POIRRIER, Anne-Lise ULiege et al

in Journal of visualized experiments : JoVE (2020), (165),

Primary Ciliary Dyskinesia (PCD) is a genetic motile ciliopathy, leading to significant otosinopulmonary disease. PCD diagnosis is often missed or delayed due to challenges with different diagnostic ... [more ▼]

Primary Ciliary Dyskinesia (PCD) is a genetic motile ciliopathy, leading to significant otosinopulmonary disease. PCD diagnosis is often missed or delayed due to challenges with different diagnostic modalities. Ciliary videomicroscopy, using Digital High-Speed Videomicroscopy (DHSV), one of the diagnostic tools for PCD, is considered the optimal method to perform ciliary functional analysis (CFA), comprising of ciliary beat frequency (CBF) and beat pattern (CBP) analysis. However, DHSV lacks standardized, published operating procedure for processing and analyzing samples. It also uses living respiratory epithelium, a significant infection control issue during the COVID-19 pandemic. To continue providing a diagnostic service during this health crisis, the ciliary videomicroscopy protocol has been adapted to include adequate infection control measures. Here, we describe a revised protocol for sampling and laboratory processing of ciliated respiratory samples, highlighting adaptations made to comply with COVID-19 infection control measures. Representative results of CFA from nasal brushing samples obtained from 16 healthy subjects, processed and analyzed according to this protocol, are described. We also illustrate the importance of obtaining and processing optimal quality epithelial ciliated strips, as samples not meeting quality selection criteria do now allow for CFA, potentially decreasing the diagnostic reliability and the efficiency of this technique. [less ▲]

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See detailThe influence of the conservation of respiratory epithelial samples on ciliary functional analysis
Bricmont, Noémie ULiege; Benchimol, Lionel ULiege; POIRRIER, Anne-Lise ULiege et al

Conference (2020)

Background: Primary ciliary dyskinesia(PCD) is an inherited otosinopulmonary ciliopathy in which respiratory cilia are stationary, or beat in a dyskinetic manner. Digital high speed videomicroscopy(DHSV ... [more ▼]

Background: Primary ciliary dyskinesia(PCD) is an inherited otosinopulmonary ciliopathy in which respiratory cilia are stationary, or beat in a dyskinetic manner. Digital high speed videomicroscopy(DHSV) allows ciliary functional analysis(CFA), including beat frequency(CBF) and pattern(CBP). DHSV is highly sensitive and specific for PCD diagnosis, but lacks standardization and evidence-based data. The technical process of ciliated epithelial samples before CFA varies among studies. Of importance, delay after sampling and temperature for samples conservation may impact CFA. However, these parameters have never been compared. Aims: To evaluate the stability of ciliary function over time when nasal brushing samples are conserved either in the fridge, or at room temperature. Methods: Ciliated epithelial samples were obtained by brushing the inferior nasal turbinate from 5 non-smoking healthy subjects. The samples were divided equally, and conserved either at 4°C or at 22°C. Beating cilia were recorded using DHSV at 37°C immediately(T0, then 9 hours after sampling(T9). CFA was assessed by CBF and the percentage of normal CBP. Results: There was no significant difference between CFA performed immediately or 9 hours after sampling, regardless of the sample storage temperature. Conclusion: This pilot study suggested that the stability of CFA may be preserved for 9 hours at 22°C and at 4°C. Larger studies are needed to confirm these preliminary results, notably in pathological conditions. [less ▲]

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See detailTranscutaneous Ultrasound for Maxillary Disease Screening in Intensive Care Unit: a Preliminary Study
Benchimol, Lionel ULiege; PIAZZA, Justine ULiege; ROGISTER, Florence ULiege et al

Conference (2020)

Introduction and Aim: Nosocomial airway infections are common and associated with high morbidity and mortality in Intensive Care Unit. Endotracheal intubation duration is an independent risk factor for ... [more ▼]

Introduction and Aim: Nosocomial airway infections are common and associated with high morbidity and mortality in Intensive Care Unit. Endotracheal intubation duration is an independent risk factor for maxillary sinusitis, which is a frequent gateway for pneumoniae, meningitis or sepsis. Diagnosis remains challenging, as CT imaging is not easily repeated in the daily assessment of unstable patients. Our aim was to evaluate the efficiency of transcutaneous ultrasound to screen maxillary sinus infection. Material and Methods: Patients presenting to our ENT department with a same-day sinus CT scan were prospectively included in this pilot study. Maxillary transcutaneous ultrasounds were performed by two independent examiners and scored in supine and sitting positions, blindly to the CT imaging, the clinical and endoscopic evaluations. Ability to diagnose sinusitis, repeatability in different positions and inter-examiners comparison were studied. Ultrasound scores were compared to Lund-Mackay-derived CT scores. Results: 7 patients (5 men and 2 women, median age 60 [56-64]) were enrolled in this experiment. In total, 14 left and right maxillary sinus were studied by same-day sinus CT scan and ultrasound in prone and sitting positions by 2 blind and independent examiners. Ultrasound was able to detect maxillary pathology the majority of the cases, with a sensitivity of 71.4% and a specificity of 87.5% in sitting position. However, sensitivity dropped to 46.8% in prone position, with a preserved specificity of 84.4%. Agreement between examiners was observed in 85.7% cases. Conclusion: Transcutaneous ultrasound was capable of screening for maxillary sinus disease compared with conventional sinus CT scan. A special attention should be paid to patient position, as sitting position improved the screening accuracy. Agreement between examiners could be improved. Moreover, despite its limitations, it is an attractive method for the screening of maxillary disease, allowing the ability to screen and/or monitor infection in unstable patients. [less ▲]

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See detailCas clinique: Dysplasie fibreuse du sinus frontal
Benchimol, Lionel ULiege

Poster (2019, April)

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See detailPrise en charge d’une méningite à Cryptococcus neoformans chez un patient VIH positif
Benchimol, Lionel ULiege

Master's dissertation (2019)

Alors que les progrès concernant le dépistage et la prise en charge des patients atteints du virus de l’immunodéficience humaine (VIH) ont été considérables, il constitue toujours à ce jour un ... [more ▼]

Alors que les progrès concernant le dépistage et la prise en charge des patients atteints du virus de l’immunodéficience humaine (VIH) ont été considérables, il constitue toujours à ce jour un problème majeur de santé public. En effet, il est estimé qu’en 2017 près d’1,8 millions de personnes ont été infectées1. Pour les patients inconscients de leur séropositivité s’installe une course contre la montre avant de développer le syndrome d’immunodéficience acquise (SIDA). Ce syndrome se définit par le développement d’une ou plusieurs infections opportunistes ou de cancers tel que le sarcome de Kaposi, apparaissant plusieurs mois ou années après l’infection par le VIH, ou un compte de lymphocyte T CD4+<200/mm3,2. Il apparait alors un défi médical d’introduire au bon moment la thérapie anti-rétrovirale (ART) sans risquer l’ «immune reconstitution inflammatory syndrome» (IRIS), source d’une mortalité importante chez les patients présentant une ou plusieurs infections opportunistes3. Il sera présenté la prise en charge d’un patient nouvellement diagnostiqué au stade SIDA (groupe C dans la classification HIV/SIDA du Center for Disease Control and Prevention) ayant développé une méningite à Cryptoccocus neoformans. Quelle est alors la stratégie thérapeutique prenant en compte à la fois l’infection opportuniste du patient et les risques de l’introduction de l’ART? C’est cette même question qui a fait débat au sein de l’équipe médicale tout au long de la prise en charge et à laquelle nous tenterons de répondre au cours de ce travail. [less ▲]

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See detailRole of Natural Killer Cells in Intravenous Immunoglobulin-Induced Graft-versus-Host Disease Inhibition in NOD/LtSz-scidIL2rg(-/-) (NSG) Mice.
Gregoire-Gauthier, Joëlle; Fontaine, francois; Benchimol, Lionel ULiege et al

in Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation (2015), 21(5), 821-8

Although clinical studies have yet to demonstrate clearly the use of intravenous immunoglobulin (IVIG) for prevention of graft-versus-host disease (GVHD), their effective use in a xenogeneic mouse model ... [more ▼]

Although clinical studies have yet to demonstrate clearly the use of intravenous immunoglobulin (IVIG) for prevention of graft-versus-host disease (GVHD), their effective use in a xenogeneic mouse model has been demonstrated. We aimed to determine the mechanism of action by which IVIG contributes to GVHD prevention in a xenogeneic mouse model. NOD/LtSz-scidIL2rg(-/-) (NSG) mice were used for our xenogeneic mouse model of GVHD. Sublethally irradiated NSG mice were injected with human peripheral blood mononuclear cells (huPBMCs) and treated weekly with PBS or 50 mg IVIG. Incidence of GVHD and survival were noted, along with analysis of cell subsets proliferation in the peripheral blood. Weekly IVIG treatment resulted in a robust and consistent proliferation of human natural killer cells that were activated, as demonstrated by their cytotoxicity against K562 target cells. IVIG treatment did not inhibit GVHD when huPBMCs were depleted in natural killer (NK) cells, strongly suggesting that this NK cell expansion was required for the IVIG-mediated prevention of GVHD in our mouse model. Moreover, inhibition of T cell activation by either cyclosporine A (CsA) or monoclonal antihuman CD3 antibodies abolished the IVIG-induced NK cell expansion. In conclusion, IVIG treatment induces NK cell proliferation, which is essential for IVIG-mediated protection of GVHD in our mouse model. Furthermore, activated T cells are mandatory for effective IVIG-induced NK cell proliferation. These results shed light on a new mechanism of action of IVIG and could explain why the efficacy of IVIG in preventing GVHD in a clinical setting, where patients receive CsA, has never been undoubtedly demonstrated. [less ▲]

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