References of "Baeten, Vincent"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailA mass spectrometry method for sensitive, specific and simultaneous detection of bovine blood meal, blood products and milk products in compound feed
Lecrenier, Marie-Caroline ULiege; Planque, Mélanie; Dieu, Marc et al

in Food Chemistry (2018), 245

Feed sustainability is one of the biggest challenges for the next few years. Solutions have to be found that take feed quality and safety into account. Animal by-products are one valuable source of ... [more ▼]

Feed sustainability is one of the biggest challenges for the next few years. Solutions have to be found that take feed quality and safety into account. Animal by-products are one valuable source of proteins. However, since the bovine spongiform encephalopathy (BSE) crisis, their use has been strictly regulated. The objective of this study was to propose a routine, sensitive and specific method using ultra-high performance liquid chromatography coupled to tandem mass spectrometry for the detection of blood-derived products and milk powder in feed. Contaminated aquafeeds were analysed in order to evaluate the sensitivity and specificity of the method. This new method meets both selectivity and sensitivity (0.1% (w/w)) requirements imposed by the European Commission for animal proteins detection methods. It offers an innovative and complementary solution for the simultaneously identification of authorised and unauthorised animal by-products such as processed animal proteins (PAPs). [less ▲]

Detailed reference viewed: 12 (4 ULiège)
Full Text
See detailUtilisation de l'imagerie hyperspectrale proche infrarouge dans l'étude de systèmes racinaires
Eylenbosch, Damien ULiege; Fernández Pierna, Juan Antonio; Baeten, Vincent et al

Conference (2018, January 25)

Lors d’études de systèmes racinaires sur base de prélèvements de carottes de sol, le tri manuel des racines et des résidus de cultures, récupérés après le lavage des échantillons, afin de les quantifier ... [more ▼]

Lors d’études de systèmes racinaires sur base de prélèvements de carottes de sol, le tri manuel des racines et des résidus de cultures, récupérés après le lavage des échantillons, afin de les quantifier, est une étape fastidieuse. Pour faciliter et accélérer cette étape, nous avons mis au point une méthode de discrimination de ces éléments sur base d’images prises en proche infrarouge. Les échantillons lavés et séchés sont passés sous une caméra qui enregistre, pour chaque pixel de l’image, un spectre en proche infrarouge. Ces spectres sont liés à la nature physico-chimique des éléments présents sur l’image et ils sont analysés à l’aide d’algorithmes d’analyses discriminantes qui permettent de les classer et donc de prédire la nature de chaque pixel de l’image. Sur base du nombre de pixels prédits comme étant des racines ou des résidus de culture, et grâce à des droites de régression reliant le nombre de pixels prédits pour ces éléments à une masse connue, nous pouvons finalement quantifier ces éléments au sein d’échantillons de sol. Cette méthode a été testée avec succès en froment et a permis de mettre très clairement en évidence l’effet du travail du sol sur le développement du système racinaire. Elle a également été utilisée sur des échantillons de sol prélevés sous des cultures associées de froment et de pois afin de distinguer et quantifier les racines des 2 espèces et les nodosités. [less ▲]

Detailed reference viewed: 16 (0 ULiège)
Full Text
See detailEtude de l’influence de la fertilisation azotée sur le développement racinaire du froment d’hiver (Triticum aestivum L.)
Eylenbosch, Damien ULiege; Fernández Pierna, Juan Antonio; Baeten, Vincent et al

Poster (2018, January)

Le poster présente les premiers résultats obtenus dans un essai sur l'effet de fertilisation azotée sur le système racinaire du froment d'hiver. La quantification des racines a été réalisée sur base d ... [more ▼]

Le poster présente les premiers résultats obtenus dans un essai sur l'effet de fertilisation azotée sur le système racinaire du froment d'hiver. La quantification des racines a été réalisée sur base d'échantillons de sol analysés, après lavage, par imagerie hyperspectrale proche infrarouge. Cette méthode d'analyse de prélèvements racinaires permet de quantifier les racines sans devoir les séparer préalablement des résidus de culture présents dans les échantillons de sol et sans devoir les peser. Pour le prélèvement racinaire réalisé au stade redressement de la culture de froment, aucun effet de la fertilisation azotée apportée au stade tallage n'a pu être mis en évidence. Une diminution de la densité racinaire sur les 90 cm de sol étudiés a par contre été clairement mesurée. [less ▲]

Detailed reference viewed: 18 (0 ULiège)
Full Text
Peer Reviewed
See detailUtilisation de l’imagerie hyperspectrale proche infrarouge combinée aux outils de la chimiométrie dans l’étude de systèmes racinaires.
Eylenbosch, Damien ULiege; Fernández Pierna, Juan Antonio; Baeten, Vincent et al

in Recueil de communications (2018, January)

In studies on root systems using the soil coring method, roots, crop residues and soil particles extracted after washing of soil samples need to be manually sorted before root quantification. If different ... [more ▼]

In studies on root systems using the soil coring method, roots, crop residues and soil particles extracted after washing of soil samples need to be manually sorted before root quantification. If different species are present in the sample, their discrimination is based on several criteria. In order to reduce the complexity and the time needed for this tedious sorting step, near infrared hyperspectral imaging was used to discriminate all these elements. Images of washed and dried samples were acquired with a camera saving, for each pixel, a near infrared spectrum. These spectra are linked to the physicochemical nature of the elements visible on the hyperspectral images and were analyzed with discriminant algorithms allowing their discrimination and to link each pixel spectrum to the nature of the elements. According to the number of pixels classified as roots and thanks to the linear relationship between the number of pixels and the root biomass in the sample, it was possible to quantify roots in soil samples without manual sorting and weighing. This methodology was successfully tested on winter wheat roots and highlighted the tillage effect on root system development. It was also tested on wheat-pea intercropping in order to quantify separately the roots of each species. [less ▲]

Detailed reference viewed: 22 (2 ULiège)
Full Text
Peer Reviewed
See detailNIR hyperspectral imaging spectroscopy and chemometrics for the discrimination of roots and crop residues extracted from soil samples
Eylenbosch, Damien ULiege; Bodson, Bernard ULiege; Baeten, Vincent et al

in Journal of Chemometrics (2017)

Roots play a major role in plant development. Their study in field conditions is important to identify suitable soil management practices for sustainable crop productions. Soil coring, which is a common ... [more ▼]

Roots play a major role in plant development. Their study in field conditions is important to identify suitable soil management practices for sustainable crop productions. Soil coring, which is a common method in root production measurement, is limited in sampling frequency due to the hand‐sorting step. This step, needed to sort roots from other elements extracted from soil cores like crop residues, is time consuming, tedious, and vulnerable to operator ability and subjectivity. To get rid of the cumbersome hand‐sorting step, avoid confusion between these elements, and reduce the time needed to quantify roots, a new procedure, based on near‐infrared hyperspectral imaging spectroscopy and chemometrics, has been proposed. It was tested to discriminate roots of winter wheat (Triticum aestivum L.) from crop residues and soil particles. Two algorithms (support vector machine and partial least squares discriminant analysis) have been compared for discrimination analysis. Models constructed with both algorithms allowed the discrimination of roots from other elements, but the best results were reached with models based on support vector machine. The ways to validate models, with selected spectra or with hyperspectral images, provided different kinds of information but were complementary. This new procedure of root discrimination is a first step before root quantification in soil samples with near‐infrared hyperspectral imaging. The results indicate that the methodology could be an interesting tool to improve the understanding of the effect of tillage or fertilization, for example, on root system development. [less ▲]

Detailed reference viewed: 8 (1 ULiège)
Full Text
See detailPlant phenotyping activities at the Walloon Agricultural Research Centre
Vincke, Damien; Durenne, Bastien ULiege; Mingeot, Dominique et al

Poster (2017, November)

Detailed reference viewed: 15 (1 ULiège)
Peer Reviewed
See detailUltra-High Performance Liquid Chromatography coupled to tandem Mass Spectrometry for the detection of animal by-products in feed
Lecrenier, Marie-Caroline ULiege; Planque, Mélanie; Dieu, Marc et al

in Book of abstracts of the 8th International Symposium on Recent advances in food analysis (2017, November)

Detailed reference viewed: 19 (2 ULiège)
Peer Reviewed
See detailSynchronous fluorescence spectroscopy for detecting blood meal and blood products in feed
Abbas, Ouissam; Lecrenier, Marie-Caroline ULiege; Baeten, Vincent

Poster (2017, September)

Detailed reference viewed: 13 (1 ULiège)
See detailEffect of grinding on bone detection by light microscopy: Study results
Lecrenier, Marie-Caroline ULiege; Veys, Pascal; Planchon, Viviane et al

Conference (2017, June)

Detailed reference viewed: 11 (1 ULiège)
Full Text
See detailQuantification of roots by the use of NIR hyperspectral imaging and chemometrics
Eylenbosch, Damien ULiege; Baeten, Vincent; Bodson, Bernard ULiege et al

Conference (2017, June)

Estimation of root system development is often based on the soil coring method which allows repeated measurements during the growing season in the field as well as in different soil horizons. However ... [more ▼]

Estimation of root system development is often based on the soil coring method which allows repeated measurements during the growing season in the field as well as in different soil horizons. However, this method is limited due to the time needed to extract roots from soil cores and to manually sort them from crop residues before quantification. To avoid this tedious sorting step and remove operator subjectivity, a procedure based on NIR Hyperspectral Imaging (NIR-HSI) has been developed. [less ▲]

Detailed reference viewed: 28 (2 ULiège)
Full Text
See detailQuantification of roots by the use of NIR hyperspectral imaging and chemometrics
Eylenbosch, Damien ULiege; Baeten, Vincent; Bodson, Bernard ULiege et al

Poster (2017, June)

Roots, the belowground part of plants, play a major role in plant development. Their study in field conditions is important to identify suitable soil management practices for suitable crop production but ... [more ▼]

Roots, the belowground part of plants, play a major role in plant development. Their study in field conditions is important to identify suitable soil management practices for suitable crop production but roots are hidden by soil and their study is therefore difficult. Estimation of root system development is often based on the soil coring method which allows repeated measurements during the growing season in the field as well as in different soil horizons. However, this method is limited due to the time needed to extract roots from soil cores and to manually sort them from crop residues before quantification. To avoid this tedious sorting step and remove operator subjectivity, a faster sorting method was developed. Near infrared hyperspectral imaging (NIR-HIS) was tested as a rapid method to quantify the amount of roots in soil samples. [less ▲]

Detailed reference viewed: 27 (3 ULiège)
Full Text
Peer Reviewed
See detailCow milk coagulation: process description, variation factors and evaluation methodologies. A review.
Troch, Thibault; Lefebure, Emilie ULiege; Baeten, Vincent et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment (2017), 21

Introduction. For dairy producers who want to transform their milk, the ability of milk to coagulate is an important parameter. It makes it possible to transform milk into cheese. Therefore, it is ... [more ▼]

Introduction. For dairy producers who want to transform their milk, the ability of milk to coagulate is an important parameter. It makes it possible to transform milk into cheese. Therefore, it is necessary to understand the coagulation process and the techniques to measure it in order to achieve the best transformation performance. The objective of this review is to describe the milk coagulation process, the factors influencing it and the methods for measuring the coagulation of milk at lab level. Literature. The processing of milk into cheese involves three steps: coagulation, dewatering and refining. Coagulation is a key step which involves the use of rennet and depends on several parameters (pH, calcium content, temperature, etc.). Some milks never coagulate. To measure the coagulation ability of milk and identify different parameters in milk coagulation properties, the Formagraph, the computerized renneting meter and the Optigraph have been developed (reference methods). Equations have been developed using infrared spectrometry to predict the parameters obtained by the reference methods. Conclusions. The milk coagulation mechanism is known. However, the issue of non-coagulating milk persists and represents a real challenge in terms of yield. The use of infrared is a faster alternative to reference methods that measure the coagulation properties of milk, but still requires an improvement in prediction equations. [less ▲]

Detailed reference viewed: 84 (13 ULiège)
See detailDetection of blood and milk in aquafeed using UHPLC MS/MS
Lecrenier, Marie-Caroline ULiege; Veys, Pascal; Fumière, Olivier et al

Conference (2017, April)

Detailed reference viewed: 14 (1 ULiège)
Full Text
Peer Reviewed
See detailSpecific detection of blood derived products in animal feed using UPLC-MS/MS
Lecrenier, Marie-Caroline ULiege; Dieu, Marc; Veys, Pascal et al

Poster (2016, November)

Blood derived products are valuable animal products used in feed for their nutritional value and their positive effects on growth and health. Nevertheless, since the BSE crisis, their use is strictly ... [more ▼]

Blood derived products are valuable animal products used in feed for their nutritional value and their positive effects on growth and health. Nevertheless, since the BSE crisis, their use is strictly regulated. Blood meal and blood products (hemoglobin powder and plasma powder) of bovine origin are totally prohibited. Blood meal and blood products of porcine origin are authorised in aquafeed, whereas only blood products are allowed to be used in feed intended for other non-ruminant. The detection of the type of protein and the species of origin is therefore crucial to ensure feed safety. With the current official methods for the detection of PAPs, light microscopy and PCR, it is not always possible to specifically identify this type of protein source. The objective of our work was to set-up a routine mass spectrometry method for a qualitative detection of blood meal and hemoglobin powder of bovine and porcine origin in feed. The method was based on the detection of species-specific peptides biomarkers identified in a previous study by a non-targeted approach. All biomarkers were peptides of α and β hemoglobin subunits. Proteins were extracted using a TCA/acetone protein precipitation protocol followed by purification with a 2-D Clean-Up Kit (GE Healthcare, USA). After an in-solution trypsin digestion step, analyses were performed by liquid chromatography (Acquity system, Waters, UK) coupled with a triple quadrupole mass spectrometer (Xevo TQS, Waters, UK) with electrospray ionisation. The acquisition and processing of data was carried out by MassLynx software (v. 4.1, Waters) and multiple reaction monitoring (MRM) design was made using the open-source software Skyline (https://skyline.gs.washington.edu/labkey/project/home/software/Skyline/begin.view). Reference hemoglobin powder was used to select MRM transitions for each peptide biomarkers and to optimise their collision energy. Commercial feed material and compound feed containing or free from blood derived products were then analysed. Selected transitions were present in materials or feed known to contained blood derived product of the same origin and absent in the others. Artificially contaminated feed with various contamination levels were also analysed in order to evaluate the influence of matrix composition and to experimentally determine the limit of detection. A first estimation of the LOD was around 0.05 % w/w which was below the LOD imposed by the EC for animal proteins detection method. [less ▲]

Detailed reference viewed: 22 (2 ULiège)
Full Text
Peer Reviewed
See detailBlood meal and blood products detection using Synchronous fluorescence spectroscopy
Lecrenier, Marie-Caroline ULiege; Abbas, Ouissam; Taira, Aurélien et al

Poster (2016, November)

In Europe, ruminant processed animal proteins (PAPs) and blood products are not allowed to be used in feed for farmed animal. In contrast, blood meal and blood products of porcine origin are both ... [more ▼]

In Europe, ruminant processed animal proteins (PAPs) and blood products are not allowed to be used in feed for farmed animal. In contrast, blood meal and blood products of porcine origin are both authorised in aqua feed, whereas only porcine blood products are allowed to be used in feed intended for other non-ruminants. Besides official methods (light microscopy and PCR), complementary methods are developed in order to refine the by-products identification. By-products derived from blood are one of these products for which additional information are needed. Indeed, these prohibited materials sometimes cannot be distinguished from those authorised (e.g. milk powder) using official methods. The aim of this work was to develop a fast and easy method to detect blood meal and blood products. This study was based on the detection of hemoglobin in animal feed by synchronous fluorescence spectroscopy (SFS). To achieve this goal, preliminary tests were carried out on reference material (hemoglobin and albumin purchased from Sigma Aldrich) in order to determine appropriate conditions (solvent) and parameters (offset values) for hemoglobin detection. Selected settings were then applied to analyse protein extracts of commercial feed material derived from blood. The results obtained on blood meal and blood products (hemoglobin powder and plasma powder) showed fluorescence spectral bands that characterise hemoglobin. In order to determine whether the method was fit for the purpose, real commercial compound feeds known to contain or to be free from blood products or blood meal were analysed. The Principal Component Analysis (PCA) applied to these spectra showed that it was possible to discriminate samples containing hemoglobin from those that do not contain. This result confirmed that SFS is a promising screening method for the detection of hemoglobin in animal feed. [less ▲]

Detailed reference viewed: 23 (1 ULiège)
See detailDetection of blood derived products in feed using targeted triple-quadrupole MS
Lecrenier, Marie-Caroline ULiege; Dieu, Marc; Veys, Pascal et al

Conference (2016, September)

Detailed reference viewed: 22 (3 ULiège)
Full Text
See detailUse of NIR hyperspectral imaging to detect and quantify nodules on root system of associated crops
Eylenbosch, Damien ULiege; Pierreux, Jérome ULiege; Fernández Pierna, Juan Antonio et al

in ESA 14 -Growing landscape - Cultivating innovative agricultural systems (2016, September)

Detailed reference viewed: 15 (3 ULiège)
Full Text
See detailUse of NIR hyperspectral imaging to detect and quantify nodules on root system of associated crops
Eylenbosch, Damien ULiege; Pierreux, Jérome ULiege; Fernández Pierna, Juan Antonio et al

Poster (2016, September)

Winter wheat and peas cultivated in association and harvested when grains are dry show promising results in temperate areas. This association reach a good land equivalent ratio, reduce the nitrogen ... [more ▼]

Winter wheat and peas cultivated in association and harvested when grains are dry show promising results in temperate areas. This association reach a good land equivalent ratio, reduce the nitrogen fertilizer needs and obtain high quality harvest. These results are probably explained by the transfer of nitrogen from peas nodules to wheat roots. To test this hypothesis, quantification of nodules is required. Visual counting of nodules is time consuming and discrimination between the two kinds of roots is almost impossible. A new method is therefore needed. Near infrared hyperspectral imaging (NIR-HIS) was tested as a rapid method to quantify the amount of nodules and roots of each species in soil samples. [less ▲]

Detailed reference viewed: 51 (11 ULiège)
Full Text
See detailApplication of NIR hyperspectral imaging combined to chemometrics to assess the impact of tillage on the root system development of a winter wheat crop
Fraipont, Guillaume ULiege; Eylenbosch, Damien ULiege; Baeten, Vincent et al

Poster (2016, July)

This poster presents de results of a study of the influence of tillage on the root development of a winter wheat crop. The originality of this research lies in the application of an innovative root ... [more ▼]

This poster presents de results of a study of the influence of tillage on the root development of a winter wheat crop. The originality of this research lies in the application of an innovative root quantification method based on the near infrared hyperspectral imaging. [less ▲]

Detailed reference viewed: 59 (9 ULiège)
Full Text
See detailLifetime stability study of a NIR hyperspectral imaging system
Eylenbosch, Damien ULiege; Bodson, Bernard ULiege; Baeten, Vincent et al

Conference (2016, July)

Detailed reference viewed: 11 (5 ULiège)