References of "Arguelles Arias, Anthony"
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See detailAstin C production by the endophytic fungus Cyanodermella asteris in planktonic and immobilized culture conditions
Vassaux, Antoine ULiege; Tarayre, Cédric; Arguelles Arias, Anthony ULiege et al

in Biotechnology Journal (2019)

The fungal endophyte Cyanodermella asteris was recently isolated from the medicinal plant Aster tataricus. This fungus produces astin C, a cyclic pentapeptide with anticancer and anti-inflammatory ... [more ▼]

The fungal endophyte Cyanodermella asteris was recently isolated from the medicinal plant Aster tataricus. This fungus produces astin C, a cyclic pentapeptide with anticancer and anti-inflammatory properties. The production of this secondary metabolite was compared in immobilized and planktonic conditions. For immobilized cultures, a stainless steel packing immersed in the culture broth was used as a support. In these conditions, the fungus exclusively grew on the packing, which provides a considerable advantage for astin C recovery and purification. C. asteris metabolism was different according to the culture conditions in terms of substrate consumption rate, cell-growth, and astin C production. Immobilized-cell cultures yielded a 30% increase of astin C production associate to a 39% increase in biomass. The inoculum type as spores rather than hyphae, and a pre-inoculation washing procedure with sodium hydroxide, turned out to be beneficial both for astin C production and fungus development onto the support. Finally, influence of culture parameters such as pH and medium composition, on astin C production was evaluated. With optimized culture conditions, astin C yield was further improved reaching a five times higher final specific yield compared to the value reported with astin C extraction from Aster tataricus (0.89 and 0.16 mg/g respectively). [less ▲]

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See detailRapid visualization of chemically related compounds using Kendrick mass defect as a filter in mass spectrometry imaging
Kune, Christopher ULiege; Mc Cann, Andréa ULiege; La Rocca, Raphaël ULiege et al

E-print/Working paper (2019)

Kendrick mass defect (KMD) analysis is widely used for helping the detection and identification of chemically related compounds based on exact mass measurements. We report here the use of KMD as a ... [more ▼]

Kendrick mass defect (KMD) analysis is widely used for helping the detection and identification of chemically related compounds based on exact mass measurements. We report here the use of KMD as a criterion for filtering complex mass spectrometry data. The method enables an automated, faster and efficient data processing, enabling the reconstruction of 2D distributions of family of homologous compound in MSI images. We show that the KMD filtering, based on a homemade software, is suitable for low resolution and high resolution MSI data. This method has been successfully applied to two different types of samples, bacteria co-cultures and brain tissue section. [less ▲]

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See detailFrom noise to signal: Kendrick mass filtering for high-resolution mass spectrometry imaging analysis
Mc Cann, Andréa ULiege; Kune, Christopher ULiege; Arguelles Arias, Anthony ULiege et al

Poster (2019, April 14)

Introduction Over the last years, lots of progress have been done in the development of mass spectrometry imaging, making the technique more and more accessible for various applications, such as ... [more ▼]

Introduction Over the last years, lots of progress have been done in the development of mass spectrometry imaging, making the technique more and more accessible for various applications, such as biomarkers discovery or bioactive compounds identification. However, the progresses made in terms of spatial and instrumental resolution has for consequences the dramatic increase of dataset size, shifting the burden from data production to data analysis. We propose here to use a semi-targeted method based on Kendrick mass defect (KMD) analysis to immediately identify the chemistry-related compounds in mass spectrometry imaging applied to microbiology samples. Thanks to an in-house software, we are now able to better understand the bacteria-bacteria interactions. Materials and methods Bacillus velezensis GA1 and Pseudomonas sp. CMR12a were inoculated on a semi-solid agar-based medium and incubated at 30°C. Region of interest was cut directly from the petri dish and transferred to the target ITO plate, previously covered with double sided conductive carbon tape. This assembly was then put in a vacuum desiccator until complete drying (overnight), and covered with HCCA matrix. Mass spectrometry images were obtained using a FT-ICR mass spectrometer (9.4T SolariX, Bruker Daltonics, Bremen, Germany). Data analysis was performed on an in-house software. Results & Discussion Thanks to the KMD analysis, we were able to directly compare and identify the nature of the compounds detected in MSI such as lipids (1) or lipopeptides (2a), without the need of an extensive database search. It was also possible to identify some lipopeptides degradation occurring nearby Pseudomonas (2b). Thanks to our in-house software, the compounds with a similar chemistry can now be filtrated and the image can be reconstructed, removing thus the noise and focusing only on the signal. [less ▲]

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See detailInfluence of the ripening stage and the lyophilization of wild cardoon flowers on their chemical composition, enzymatic activities of extracts and technological properties of cheese curds
Ben Amira, Amal; Blecker, Christophe ULiege; Richel, Aurore ULiege et al

in Food Chemistry (2018), 245

The selection of an appropriate Cynara cardunculus flowers batch is a preliminary step to produce rennet with better clotting properties. For this reason, we proposed to study the influence of the ... [more ▼]

The selection of an appropriate Cynara cardunculus flowers batch is a preliminary step to produce rennet with better clotting properties. For this reason, we proposed to study the influence of the ripening stage and the lyophilization of cardoon flowers on their chemical composition, enzymatic activities of extracts, and technological properties of cheese curds. Results of flowers composition have shown that lyophilized flowers harvested at the middle of ripening stage (A) could be employed to produce mainly proteins or milk-clotting proteases. To confirm this, enzymatic activities of extracts and technological properties of curds were assessed. The experimental findings revealed that flowers lyophilization seems to be an efficient way to produce rennet with better clotting properties, leading to higher yield, moisture, and texture parameters of curd. These findings allowed us to select lyophilized flowers (A) for further cheese making process. [less ▲]

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See detailGenetic engineering of the branched fatty acid metabolic pathway of Bacillus subtilis for the overproduction of surfactin C14 isoform
Dhali, D.; Coutte, F.; Arguelles Arias, Anthony ULiege et al

in Biotechnology Journal (2017), 12(7),

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See detailStimulation of Fengycin-Type Antifungal Lipopeptides in Bacillus amyloliquefaciens in the Presence of the Maize Fungal Pathogen Rhizomucor variabilis.
Zihalirwa Kulimushi, Parent; Arguelles Arias, Anthony ULiege; Franzil, Laurent et al

in Frontiers in Microbiology (2017), 8

Most isolates belonging to the Bacillus amyloliquefaciens subsp. plantarum clade retain the potential to produce a vast array of structurally diverse antimicrobial compounds that largely contribute to ... [more ▼]

Most isolates belonging to the Bacillus amyloliquefaciens subsp. plantarum clade retain the potential to produce a vast array of structurally diverse antimicrobial compounds that largely contribute to their efficacy as biocontrol agents against numerous plant fungal pathogens. In that context, the role of cyclic lipopeptides (CLPs) has been well-documented but still little is known about the impact of interactions with other soil-inhabiting microbes on the expression of these molecules. In this work, we wanted to investigate the antagonistic activity developed by this bacterium against Rhizomucor variabilis, a pathogen isolated from diseased maize cobs in Democratic Republic of Congo. Our data show that fengycins are the major compounds involved in the inhibitory activity but also that production of this type of CLP is significantly upregulated when co-cultured with the fungus compared to pure cultures. B. amyloliquefaciens is thus able to perceive fungal molecules that are emitted and, as a response, up-regulates the biosynthesis of some specific components of its antimicrobial arsenal. [less ▲]

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See detailGrowth of desferrioxamine deficient Streptomyces mutants through xenosiderophore piracy of airborne fungal contaminations
Arguelles Arias, Anthony ULiege; Lambert, Stephany; Martinet, Loïc et al

in FEMS Microbiology Ecology (2015)

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See detailIdentification, production et caractérisation de l’amylolysine, un lantibiotique de Type-B produit par Bacillus amyloliquefaciens GA1
Arguelles Arias, Anthony ULiege

Doctoral thesis (2014)

Bacillus amyloliquefaciens GA1, antérieurement dénommée Bacillus subtilis GA1, est décrit dans la littérature comme étant une souche capable d’inhiber la croissance de nombreux micro-organismes. Afin ... [more ▼]

Bacillus amyloliquefaciens GA1, antérieurement dénommée Bacillus subtilis GA1, est décrit dans la littérature comme étant une souche capable d’inhiber la croissance de nombreux micro-organismes. Afin d’identifier les molécules responsables de ces activités biologiques, un séquençage partiel et aléatoire du génome de cette souche a été entrepris au laboratoire. Parallèlement à ce séquençage, nous avons entrepris une caractérisation globale des propriétés biologiques des métabolites secondaires produits par cette souche. La production de ces métabolites, essentiellement de type PKs et NRPs, a été mise en évidence par chromatographie liquide couplée à la spectrométrie de masse (LC-MS). Ainsi, nous avons pu mettre en évidence la présence de plusieurs clusters de gènes mais également la production de lipopeptides, polykétides et sidérophores. Lors du séquençage partiel du génome de B. amyloliquefaciens GA1, l’identification d’une séquence de 800 paires de bases (bp) homologue à celle d’un gène de Bacillus licheniformis ATCC 14580 codant pour une enzyme de modification d’un lantibiotique (« Lanthionine containing antibiotic »), nous a permis d’émettre l’hypothèse que B. amyloliquefaciens GA1 pourrait produire une molécule de type lantibiotique. Afin de vérifier cette hypothèse, nous avons entrepris l’identification, la purification et la caractérisation de ce peptide antibactérien. Pour permettre cette caractérisation, nous avons tout d’abord mis au point une méthode de production et de purification adaptée aux lantibiotiques. En parallèle, un séquençage des gènes responsables de la biosynthèse de ce lantibiotique, dénommé amylolysine, a également été réalisé. Les gènes responsables de la biosynthèse du lantibiotique sont au nombre de sept, formant quatre opérons, pour une taille totale de 9,6 kilobases (kb). La masse moléculaire du lantibiotique purifié, ainsi que les gènes de biosynthèse de celui-ci, nous ont confirmé que le lantibiotique produit par B. amyloliquefaciens GA1 est un lantibiotique qui n’avait pas encore été décrit dans la littérature. Nous avons mis en évidence le mode d’action de cette bactériocine de Type 1 et initier une étude structurale de cette dernière. Nos résultats ont démontrés que l’amylolysine est un lantibiotique de Type-B bien que son mode d’action soit atypique pour ce type de molécule. En effet, l’amylolysine est capable, à la fois, d’interagir avec le lipide II, ce qui inhibe la synthèse du peptidoglycane, et de former des pores dans la membrane des bactéries sensibles. Ce double mode d’action, décrit chez les lantibiotiques de Type-A, n’a jamais été décrit chez les lantibiotiques de Type-B, qui, en général ne sont pas capables de former des pores dans la membrane des bactéries. Enfin, dans le but de démontrer une application biotechnologique de l’amylolysine, sa capacité à inhiber la croissance de souches pathogènes, tel que Listeria monocytogenes, sur de la viande de volaille a été démontrée et comparée à la nisine, un lantibiotique utilisé en tant qu’agent de conservation dans l’industrie agro-alimentaire depuis de nombreuses années. En plus d’inhiber la croissance de L. monocytogenes, nous avons démontré que l’amylolysine est plus stable que la nisine par rapport aux protéases présentes naturellement dans la viande de volaille. Ce qui fait de l’amylolysine un excellent candidat pour l’utilisation de cette dernière en tant qu’agent de conservation. [less ▲]

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See detailISOLATION OF THE ANTIMICROBIAL CYCLIC PEPTIDE SUBTILOSIN A FROM A GUT-ASSOCIATED BACILLUS SUBTILIS STRAIN
Schyns, Ghislain; Serra, Claudia; Henriques, Adriano et al

in American Journal of Biochemistry and Biotechnology (2013), 9(3),

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See detailAntimicrobial Activity of Bacillus amyloliquefaciens ANT1 Toward Pathogenic Bacteria and Mold: Effects on Biofilm Formation
Arguelles Arias, Anthony ULiege; Nastro, Rosa Anna; Ongena, Marc ULiege et al

in Probiotics and Antimicrobial Proteins (2013)

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See detailSynthesis and biological evaluation of potential threonine synthase inhibitors: Rhizocticin A and Plumbemycin A.
Gahungu, Mathias; Arguelles Arias, Anthony ULiege; Fickers, Patrick ULiege et al

in Bioorganic and Medicinal Chemistry (2013), 21(17), 4958-67

Rhizocticins and Plumbemycins are natural phosphonate antibiotics produced by the bacterial strains Bacillus subtilis ATCC 6633 and Streptomyces plumbeus, respectively. Up to now, these potential ... [more ▼]

Rhizocticins and Plumbemycins are natural phosphonate antibiotics produced by the bacterial strains Bacillus subtilis ATCC 6633 and Streptomyces plumbeus, respectively. Up to now, these potential threonine synthase inhibitors have only been synthesized under enzymatic catalysis. Here we report the chemical stereoselective synthesis of the non-proteinogenic (S,Z)-2-amino-5-phosphonopent-3-enoic acid [(S,Z)-APPA] and its use for the synthesis of Rhizocticin A and Plumbemycin A. In this work, (S,Z)-APPA was synthesized via the Still-Gennari olefination starting from Garner's aldehyde. The Michaelis-Arbuzov reaction was used to form the phosphorus-carbon bond. Oligopeptides were prepared using liquid phase peptide synthesis (LPPS) and were tested against selected bacteria and fungi. [less ▲]

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See detailCharacterization of amylolysin, a novel lantibiotic from Bacillus amyloliquefaciens GA1
Arguelles Arias, Anthony ULiege; Ongena, Marc ULiege; Devreese, Bart et al

in PLoS ONE (2013), 8(12),

Background: Lantibiotics are heat-stable peptides characterized by the presence of thioether amino acid lanthionine and methyllanthionine. They are capable to inhibit the growth of Gram-positive bacteria ... [more ▼]

Background: Lantibiotics are heat-stable peptides characterized by the presence of thioether amino acid lanthionine and methyllanthionine. They are capable to inhibit the growth of Gram-positive bacteria, including Listeria monocytogenes, Staphylococcus aureus or Bacillus cereus, the causative agents of food-borne diseases or nosocomial infections. Lantibiotic biosynthetic machinery is encoded by gene cluster composed by a structural gene that codes for a pre-lantibiotic peptide and other genes involved in pre-lantibiotic modifications, regulation, export and immunity. Methodology/Findings: Bacillus amyloliquefaciens GA1 was found to produce an antimicrobial peptide, named amylolysin, active on an array of Gram-positive bacteria, including methicillin resistant S. aureus. Genome characterization led to the identification of a putative lantibiotic gene cluster that comprises a structural gene (amlA) and genes involved in modification (amlM), transport (amlT), regulation (amlKR) and immunity (amlFE). Disruption of amlA led to loss of biological activity, confirming thus that the identified gene cluster is related to amylolysin synthesis. MALDI-TOF and LC-MS analysis on purified amylolysin demonstrated that this latter corresponds to a novel lantibiotic not described to date. The ability of amylolysin to interact in vitro with the lipid II, the carrier of peptidoglycan monomers across the cytoplasmic membrane and the presence of a unique modification gene suggest that the identified peptide belongs to the group B lantibiotic. Amylolysin immunity seems to be driven by only two AmlF and AmlE proteins, which is uncommon within the Bacillus genus. Conclusion/Significance: Apart from mersacidin produced by Bacillus amyloliquefaciens strains Y2 and HIL Y-85,544728, reports on the synthesis of type B-lantibiotic in this species are scarce. This study reports on a genetic and structural characterization of another representative of the type B lantibiotic in B. amyloliquefaciens. Copyright: © 2013 Arguelles Arias et al. [less ▲]

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See detailGram-positive antibiotic biosynthetic clusters: a review
Arguelles Arias, Anthony ULiege

in Science against Microbial Pathogens: Communicating Current Research and Technological Advances (2011)

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