References of "Antoine, Nadine"
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See detailHistologie générale - Exercices et méthodes
Thiry, Marc ULiege; Antoine, Nadine ULiege; Caudroy, Stéphanie et al

Book published by DUNOD (2019)

Cet ouvrage propose aux étudiants des premières années d’études supérieures (Licence 2 et 3) une méthode progressive et efficace pour comprendre et appliquer les concepts fondamentaux de l'histologie. Il ... [more ▼]

Cet ouvrage propose aux étudiants des premières années d’études supérieures (Licence 2 et 3) une méthode progressive et efficace pour comprendre et appliquer les concepts fondamentaux de l'histologie. Il traite de l'histologie des tissus sains et adultes chez les mammifères, Homme inclus. À la suite de rappels de cours, sous forme de fiches, chaque chapitre propose des exercices de difficulté croissante pour s’évaluer : QCM, questions Vrai/Faux et exercices de synthèse. Les corrigés détaillés mettent en évidence la méthodologie. Des bonus web avec des exercices d’entraînement supplémentaires, des fiches sur les techniques de préparation des coupes histologiques et des photos de coupes histologiques en couleur complètent l’ouvrage. [less ▲]

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See detailInvestigation of serum Krebs Von den Lungen 6 concentration as a predisposing factor and in the diagnosis of canine idiopathic pulmonary fibrosis in the West Highland white terrier.
Fastrès, Aline ULiege; Roels, Elodie ULiege; Bolen, Géraldine ULiege et al

Poster (2018, September 06)

Canine idiopathic pulmonary fibrosis (CIPF) mimics human idiopathic pulmonary fibrosis (IPF) and affects old dogs from the West Highland white terrier (WHWT) breed. Due to difficulties to both diagnose ... [more ▼]

Canine idiopathic pulmonary fibrosis (CIPF) mimics human idiopathic pulmonary fibrosis (IPF) and affects old dogs from the West Highland white terrier (WHWT) breed. Due to difficulties to both diagnose early and follow the course of pulmonary fibrosis, numerous biomarkers have been studied in both species. In humans, the mucin Krebs Von den Lungen 6 (KL-6) has consistently been found to be increased in the serum from patients with interstitial lung disease, including IPF and is possibly involved in the IPF pathophysiological process. In CIPF, it has not yet been assessed. In this study, we assessed the diagnostic value of serum KL-6 concentration in CIPF as well as the association between serum KL-6 concentrations and breed predisposition for the disease. Blood was obtained from 5 groups of dogs: WHWTs affected with CIPF (n=20, 14 females (F)/6 males (M), mean age=11.0y, range [5.2-14.5]), healthy aged-matched WHWTs (n=18, 11F/7M, 10.5y [5.3-15.3]), and healthy dogs from other breeds not predisposed for CIPF: Jack Russel terriers (JR) (n=13, 8F/5M, 6.0y [3.0-9.1]), Malinois Belgian shepherds (MBS) (n=13, 5F/8M, 6.5y [2.8-10.8]) and King Charles spaniels (KCS) (n=13, 9F/4M, 6.5y [3.6-9.3]). Serum was stored at -80°C until batched analysis. KL-6 concentration was measured with a commercially available ELISA test (Canine Krebs Von den Lungen 6 Elisa kit, amsbio). Results were compared using a covariance analysis including the 5 groups defined above, gender and an interaction between groups and gender together with the effect of the age. The analysis was followed by post-hoc comparisons between groups using t-tests. Results show that only the effect of groups was significant (p=0.010). Serum KL-6 concentration was higher in healthy WHWTs (4.8+/-4.0 ng/ml) compared with KCS (1.6+/-1.8 ng/ml) and MBS (2.2+/-1.0 ng/ml) (p=0.001 and 0.013 respectively) and higher in JR (4.1+/-2.9 ng/ml) compared with KCS (p=0.016). There was no difference between CIPF (3.2+/-1.9 ng/ml) and healthy WHWTs (p=0.072). In conclusion, differences in serum KL-6 concentrations were found amongst breeds in healthy dogs, with higher concentrations observed in terrier breeds. Whether this may reflect a predisposing factor for CIPF development merits further investigations. Unlike what has been found in human IPF, KL-6 is not a good serum biomarker for diagnosis as there was no difference between CIPF and unaffected WHWTs. [less ▲]

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See detailA tutored online remedial course in veterinary Histology: For which students? For which perceptions and results?
Tonus, Céline ULiege; Jérôme, Françoise ULiege; Antoine, Nadine ULiege

Poster (2018, May)

Introduction and aim of the study. An online remedial course was associated to Histology practical teaching in the third year of the Bachelor's degree in the Faculty of Veterinary Medicine of the ... [more ▼]

Introduction and aim of the study. An online remedial course was associated to Histology practical teaching in the third year of the Bachelor's degree in the Faculty of Veterinary Medicine of the University of Liège. The main characteristics of this program were (i) voluntary enrolment, (ii) structured and progressive learning, (iii) individualized guidance and (iv) the opportunity for each student to work at his/her own pace. For each organ system, three levels of exercises were developed by combining the institutional e-learning environment (eCampus) and the virtual microscopy application Cytomine/Shareview. Level 1 consisted in short exercises aiming to revise the theoretical notions essential to the proper understanding of histological observations. Level 2 consisted in complete and guided description of histological slides very similar to those observed during in-class practical labs. Level 3 consisted in identification and description of unknown histological slides. The present study aimed at determining the learning profiles of participants in the remedial course and at making out the course’s effects on each profile in terms of engagement, perceptions and performance. Material and methods. Engagement and performance of each student were recorded during in-class practical labs and during remedial course. At the end of the remedial course and before summative assessment, students' perception was evaluated by an on-line survey. Performance to the summative assessment was also recorded. Results. Despite the small number of participants (n=22), four learning profiles could be determined. Profiles differ from each other essentially in terms of students’ strategic choices when entering the course and regarding their engagement in remedial exercises. Students' perceptions in relation to the remedial course were generally positive, regardless of the learning strategy they adopted. Effects on performance in summative assessment were only observable for students who developed engaged or strategical learning profiles. [less ▲]

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See detailA Preliminary Study Into Meniscal Innervation in 2 Horses With Different Degrees of Chondropathy in the Femorotibial Joint
Nemery, Elodie ULiege; Gabriel, Annick ULiege; Moyse, Evelyne ULiege et al

in Journal of Equine Veterinary Science (2018)

Osteoarthritis is a painful degenerative disease, sometimes responsible for the premature ending of the careers of sporting horses. In humans, meniscal neurogenesis has been suggested to contribute to ... [more ▼]

Osteoarthritis is a painful degenerative disease, sometimes responsible for the premature ending of the careers of sporting horses. In humans, meniscal neurogenesis has been suggested to contribute to pain in osteoarthritis. To the best of our knowledge, no study on this subject has been performed in equine species. The aim of this report was to compare the density of the nociceptive and sympathetic nerves in the menisci of two 11- and 13-year-old mares, one presenting a “low-chondropathy grade” in its stifle and the other a “high-chondropathy grade”. The cranial horns of the medial menisci, a commonly reported site of equine meniscal injuries, were harvested after a maximum of 48 hours postmortem. A macroscopic and microscopic grading system was established using a modified Pauli score. Nociceptive fibers were highlighted by immunohistochemical staining with an antibody against Substance P (SP) and postganglionic sympathetic fibers with an antibody against tyrosine hydroxylase (TH). Descriptive statistics were calculated, and a nonparametric statistical test was used to analyze the data. The meniscus from the “high-chondropathy horse (HCH)” showed a higher density and frequency of postganglionic sympathetic nerves compared with the meniscus of the “low-chondropathy horse (LCH)”. No difference was observed regarding nociceptive nerves. This report shows possible meniscal sympathetic efferent-mediated pain in a “highly chondropathic horse”. Although these results cannot be extended to the equine population as a whole because of the small sample size, they are nevertheless interesting as they provide new information regarding the possible etiology of the pain in equine stifle osteoarthritis. [less ▲]

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See detailLa microscopie virtuelle
Pesesse, Laurence ULiege; Weatherspoon, Alodie ULiege; Defaweux, Valérie ULiege et al

Conference (2017, November 23)

La microscopie virtuelle fait partie intégrante des activités d'enseignement de l'Histologie et de l’Histopathologie Humaine et Animale que ce soit lors de travaux pratiques en présentiel, à distance ... [more ▼]

La microscopie virtuelle fait partie intégrante des activités d'enseignement de l'Histologie et de l’Histopathologie Humaine et Animale que ce soit lors de travaux pratiques en présentiel, à distance, dans l'animation d'un MOOC ou encore lors de cours théoriques ex-cathedra. L'interface web de Cytomine permet de donner la parole aux lames de tissus digitalisés en y associant un langage, qu'il soit descriptif sous forme d'annotations, didactique par le biais d'explications liant structure et fonctions, interrogatif en y associant des questions ou sous la forme d'un dialogue grâce au partage possible avec d'autres utilisateurs. Différents scénarios d'apprentissage élaborés par la Faculté de Médecine et de Médecine Vétérinaire vous exposeront les atouts de la microscopie virtuelle et comment celle-ci est devenue un outil incontournable de travail actif et collaboratif. [less ▲]

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See detailCryopreservation of chicken primordial germ cells by vitrification and slow-freezing: a comparative study
Tonus, Céline ULiege; Connan, Delphine ULiege; Waroux, Olivier ULiege et al

in Theriogenology (2017), 88

In the present study, we compare a classical slow freezing method and an aseptic vitrification technique to cryopreserve a stable Primordial Gem Cells (PGCs) line issued from the Ardennaise chicken breed ... [more ▼]

In the present study, we compare a classical slow freezing method and an aseptic vitrification technique to cryopreserve a stable Primordial Gem Cells (PGCs) line issued from the Ardennaise chicken breed. Viability immediately after warming was close to 80% and did not differ between the two cryopreservation methods. Proliferation tended to be slower for both cryopreservation methods compared to controls, but the difference was significant only for vitrification. No difference was found between the two methods after flow cytometry analysis of SSEA-1 expression and RT-PCR on several factors related to PGCs phenotype. After one week in culture, all cryopreserved cells reached controls main morphological and expanding (viability/proliferation) features. However, slow freezing generated more unwanted cells clusters than vitrification. After injection of the PGCs into recipient embryos, vitrified PGCs showed a clear, yet not significant, tendency to colonize the gonad at a higher rate than slow frozen PGCs. Slow freezing in cryovials remains simple, inexpensive and less technically demanding than vitrification. Nevertheless, the intrinsic advantages of our aseptic vitrification method and the present study suggest that this should be considered as safer than classical slow freezing for cryopreserving chicken PGCs. [less ▲]

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See detailVitriCell: the safest key for cryopreserving your valuable cells
Connan, Delphine; Ectors, Fabien ULiege; Vanderzwalmen, Pierre ULiege et al

Poster (2016, December 08)

VITRICELL is a future spin-off company aiming at providing standard and customized solutions for safe and efficient cryopreservation of valuable and sensible eukaryotic cells. Developed in the Embryology ... [more ▼]

VITRICELL is a future spin-off company aiming at providing standard and customized solutions for safe and efficient cryopreservation of valuable and sensible eukaryotic cells. Developed in the Embryology unit of the Faculty of Veterinary Medicine of the University of Liège, the core methodology relies on aggregation of inventor’s pioneering expertises in the fields of stem cells engineering, vitrification of gametes and embryos and in assisted reproductive technologies. VITRICELL will soon provide researchers and clinicians with vitrification kits, allowing upgrade of the current yields and safety after cryopreservation of their high-value cells. The benefits of our method are the most prominent with fragile cell lines limited in expansion ability. VITRICELL’s current product is based on a novel and user-friendly, aseptic and automatable vitrification method in sealed french straws, with bio-safe and chemically defined media. The absence of any ice crystal formation avoids excessive cell dehydration and injuries to cell membranes. As a consequence, VITRICELL’s method results in a higher efficiency (recovery rates, morphology, pluripotency…) than conventional slow freezing for cryopreserving human pluripotent stem cells (hPSCs) and other sensitive stem cell-like lines and embryos from human and non-human species. VITRICELL’s future products developments focuses on (i) vitrification of higher cells numbers in larger containers, (ii) implementation of the method to current automation processes. [less ▲]

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See detailVITRICELL: new efficient method for cryopreserving cells by vitrification
Connan, Delphine ULiege; Ectors, Fabien ULiege; Antoine, Nadine ULiege et al

Conference (2016, September)

Using stem and related cells for clinical purposes requires efficient and bio-safe handling. Cryopreservation is a mandatory key step of storage and transportation, during which cells undergo extreme ... [more ▼]

Using stem and related cells for clinical purposes requires efficient and bio-safe handling. Cryopreservation is a mandatory key step of storage and transportation, during which cells undergo extreme physical and chemical conditions prone to alter their viability as well as their biological properties. Conventional slow-freezing often results in poor survival rates mainly due to excessive cell dehydration and water crystallization. We have addressed this problem by developing a new cryopreservation method based on aseptic and automatable vitrification in sealed french straws. Furthermore, only bio-safe and chemically defined cryopreservation media are used. We have demonstrated that, despite additional constraints, our aseptic vitrification process is more efficient (recovery rates, morphology, pluripotency…) than conventional slow freezing for cryopreserving human pluripotent stem cells (hPSCs). These results have been confirmed on various sensitive stem cell-like lines and embryos from human and non-human species. VITRICELL will soon provide researchers and clinicians with its vitrification kits, allowing to upgrade the current yields and safety after cryopreservation of their high-value cells. [less ▲]

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See detailThermosensitivity of the sex differentiation process in the African catfish, Clarias gariepinus: Determination of the thermosensitive period
Santi, Saïdou ULiege; Gennotte, Vincent ULiege; Toguyeni, Aboubacar et al

in Aquaculture (2016), 455

Gonadal sex differentiation in gonochoristic fish is generally labile and under the control of two interacting processes: genetic sex determination (GSD) and environmental sex determination (ESD ... [more ▼]

Gonadal sex differentiation in gonochoristic fish is generally labile and under the control of two interacting processes: genetic sex determination (GSD) and environmental sex determination (ESD). Numerous experimental studies deal with temperature induced-sex differentiation in teleosts, but none focused on the African catfish Clarias gariepinus. The aim of this study was to confirm the thermosensitivity of the sex differentiation process and to determine the thermosensitive period during the African catfish development. Fish were exposed to high temperature (36 °C) for 3 days at different periods during ontogenesis. The treatment was applied every 3 days from fertilization until 29 days post-hatching (dph). Before and after the thermal treatment, fish were reared at 28 °C. Gonadal development was histologically characterized on fish sampled at 10, 15, 20, 25, 35, 45, 55 and 70 dph. Our results demonstrated that the African catfish displays a thermosensitivity of the sex differentiation process, with a masculinizing effect of high temperature (36 °C). The most thermosensitive period extended from 6 to 8 dph. Fish batches exposed to 36 °C during this period showed a sex-ratio skewed towards the male phenotype, ranging from 58 to 100% (high inter-familial variability). The African catfish gonads (male and female) stayed histologically undifferentiated until 20 dph. Obvious signs of gonadal differentiation clearly appeared at 25 dph in females and at 45 dph in males. Variability in sex-ratios between progenies and in the response to thermal treatment suggests a role of minor genetic factors and interactions between genomic and environmental determinants in the expression of the sexual phenotype. Statement of relevance: 1. This paper provides novel methods to control African catfish Clarias gariepinus sex differentiation through high temperature exposure and then to produce all-male populations. 2. Our work underlines the possibility to significantly reduce high temperature (masculinizing effect) treatment period to 3 days and consequently increase survival rate of progenies after treatment. 3. This study also shows the inter-family variability of thermosensitivity on the sex differentiation process in Clarias gariepinus. [less ▲]

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See detailGuanine nucleotide exchange factor RABGEF1 regulates keratinocyte-intrinsic signaling to maintain skin homeostasis.
Marichal, Thomas ULiege; Gaudenzio, Nicolas; El Abbas, Sophie ULiege et al

in Journal of Clinical Investigation (2016), 126(12), 4497-4515

Epidermal keratinocytes form a structural and immune barrier that is essential for skin homeostasis. However, the mechanisms that regulate epidermal barrier function are incompletely understood. Here we ... [more ▼]

Epidermal keratinocytes form a structural and immune barrier that is essential for skin homeostasis. However, the mechanisms that regulate epidermal barrier function are incompletely understood. Here we have found that keratinocyte-specific deletion of the gene encoding RAB guanine nucleotide exchange factor 1 (RABGEF1, also known as RABEX-5) severely impairs epidermal barrier function in mice and induces an allergic cutaneous and systemic phenotype. RABGEF1-deficient keratinocytes exhibited aberrant activation of the intrinsic IL-1R/MYD88/NF-kappaB signaling pathway and MYD88-dependent abnormalities in expression of structural proteins that contribute to skin barrier function. Moreover, ablation of MYD88 signaling in RABGEF1-deficient keratinocytes or deletion of Il1r1 restored skin homeostasis and prevented development of skin inflammation. We further demonstrated that epidermal RABGEF1 expression is reduced in skin lesions of humans diagnosed with either atopic dermatitis or allergic contact dermatitis as well as in an inducible mouse model of allergic dermatitis. Our findings reveal a key role for RABGEF1 in dampening keratinocyte-intrinsic MYD88 signaling and sustaining epidermal barrier function in mice, and suggest that dysregulation of RABGEF1 expression may contribute to epidermal barrier dysfunction in allergic skin disorders in mice and humans. Thus, RABGEF1-mediated regulation of IL-1R/MYD88 signaling might represent a potential therapeutic target. [less ▲]

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See detailLong term-cultured and cryopreserved primordial germ cells from various chicken breeds retain high proliferative potential and gonadal colonisation competency
Tonus, Céline ULiege; Cloquette, Karine; Ectors, Fabien ULiege et al

in Reproduction, Fertility and Development (2016), 28(5), 628-639

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See detailNociceptive and sympathetic innervations in the abaxial part of the cranial horn of the equine medial meniscus: an immunohistochemical approach
Nemery, Elodie ULiege; Gabriel, Annick ULiege; Piret, Joëlle ULiege et al

in Journal of Anatomy (2016)

In athletic horses, diseases leading to lameness are of major importance due to the loss of performance and the resultant economic concerns. Although, in the hindlimb, stifle lesions are frequent due to ... [more ▼]

In athletic horses, diseases leading to lameness are of major importance due to the loss of performance and the resultant economic concerns. Although, in the hindlimb, stifle lesions are frequent due to the large and complex joint, and meniscal tears have been identified as the most common soft tissue injuries in this joint, little is known about the mechanism that causes the painful sensation and thus the lameness. The aim of our study was therefore to highlight any peripheral fibres involved in meniscal nociception in five healthy anterior horns of the equine medial meniscus, which is the most common locale reported for equine meniscal injuries. Immunohistochemical stainings were performed using antibodies against Substance P in order to identify nociceptive fibres; antibodies against tyrosine hydroxylase for detecting postganglionic sympathetic fibres; and against glial fibrillary acidic proteins in order to identify Schwann cells. Fundamentally, our work highlights for the first time the presence of nociceptive and sympathetic fibres in equine menisci. They were found in the abaxial part of the anterior horn of the equine medial meniscus. This study suggests thus that when the abaxial part is injured, the meniscus itself could be the source of pain. This finding provides a better understanding of the nociceptive and sympathetic innervation of the equine meniscus which could contribute towards improving the therapeutic strategies in order to alleviate pain in cases of equine meniscal injury. [less ▲]

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See detailMechanoreceptors in the anterior horn of the equine medial meniscus: an immunohistochemical approach
Nemery, Elodie ULiege; Gabriel, Annick ULiege; Grulke, Sigrid ULiege et al

in Anatomia, Histologia, Embryologia (2016), 45

Lameness due to stifle and especially meniscal lesions is frequent in equine species. In humans, mechanoreceptors involved in proprioceptive function are well studied. Given the high incidence of meniscal ... [more ▼]

Lameness due to stifle and especially meniscal lesions is frequent in equine species. In humans, mechanoreceptors involved in proprioceptive function are well studied. Given the high incidence of meniscal injuries in horses, and the lack of information concerning them in equine menisci, our objective was to study these corpuscles in six healthy anterior horns of the equine medial meniscus, which is the most common localisation reported for equine meniscal injuries. Immunohistochemical stainings were performed using antibodies against high molecular weight neurofilaments and glial fibrillary acidic proteins. From a purely fundamental point of view, our work highlights for the first time the presence of Ruffini, Pacini and Golgi corpuscles in equine meniscus. They were found, isolated or in clusters and always located at the vicinity of blood vessels, at the level of the anterior horn of the equine medial meniscus. This morphological approach could serve as a basis for clinical studies, to evaluate the impact of these corpuscles on the poor sportive prognosis in equine meniscal tears. [less ▲]

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See detailEquine cadaver ligaments : A new promising source of stem cells
Shikh Al Sook, Mohamad Khir ULiege; Gabriel, Annick ULiege; Salouci, Moustafa et al

Scientific conference (2015, November 07)

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