References of "Amory, Hélène"
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See detailComparison of Fecal Microbiota of Horses Suffering from Atypical Myopathy and Healthy Co-Grazers
Wimmer-Scherr, Christina ULiege; Taminiau, Bernard ULiege; Renaud, Benoît ULiege et al

in Animals MPDI (2021), 11(506),

Equine atypical myopathy (AM) is caused by hypoglycin A (HGA) and methylenecyclopropylglycine (MCPG) intoxication resulting from the ingestion of seeds or seedlings of some Acer tree species ... [more ▼]

Equine atypical myopathy (AM) is caused by hypoglycin A (HGA) and methylenecyclopropylglycine (MCPG) intoxication resulting from the ingestion of seeds or seedlings of some Acer tree species. Interestingly, not all horses pasturing in the same toxic environment develop signs of the disease. In other species, it has been shown that the intestinal microbiota has an impact on digestion, metabolism, immune stimulation and protection from disease. The objective of this study was to characterize and compare fecal microbiota of horses suffering from AM and healthy co-grazers. Furthermore, potential differences in fecal microbiota regarding the outcome of diseased animals were assessed. This prospective observational study included 59 horses with AM (29 survivors and 30 nonsurvivors) referred to three Belgian equine hospitals and 26 clinically healthy co-grazers simultaneously sharing contaminated pastures during spring and autumn outbreak periods. Fresh fecal samples (rectal or within 30min of defecation) were obtained from all horses and bacterial taxonomy profiling obtained by 16S amplicon sequencing was used to identify differentially distributed bacterial taxa between AM-affected horses and healthy co-grazers. Fecal microbial diversity and evenness were significantly (p < 0.001) higher in AM-affected horses as compared with their non-affected co-grazers. The relative abundance of families Ruminococcaceae, Christensenellaceae and Akkermansiaceae were higher (p ≤ 0.001) whereas those of the Lachnospiraceae (p = 0.0053), Bacteroidales (p < 0.0001) and Clostridiales (p = 0.0402) were lower in horses with AM, especially in those with a poor prognosis. While significant shifts were observed, it is still unclear whether they result from the disease or might be involved in the onset of disease pathogenesis. [less ▲]

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See detailHigh Seroprevalence Against Lawsonia intracellularis Among Adult Horses in Belgium
Loublier, Clémence ULiege; Cerri, Simona; Gryspeerdt, Annick et al

in Journal of Equine Veterinary Science (2020), 95

Lawsonia intracellularis (LI) is an obligate intracellular gram-negative rod causing equine proliferative enteropathy (EPE). Occasional cases of EPE have been reported in foals living in Belgium, but the ... [more ▼]

Lawsonia intracellularis (LI) is an obligate intracellular gram-negative rod causing equine proliferative enteropathy (EPE). Occasional cases of EPE have been reported in foals living in Belgium, but the seroprevalence of equine LI in this country is unknown. The target population included clinically healthy adult horses, whose blood samples were collected and analysed for specific IgG antibodies against LI using a blocking enzyme-linked immunosorbent assay (bELISA) test. Results were expressed as Percentage of Inhibition (PI). Samples that had a PI <20% were judged as negative, those between 20 and 30% as inconclusive and those >30% were considered positive. A total of 356 blood samples were analysed with 352 horses (98.8%) testing positive, 2 horses (0. 6%) testing negative and 2 horses (0.6%) showing inconclusive results. The large percentage of seropositive samples obtained in this study confirms a widespread exposure of Belgian horses to LI. [less ▲]

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See detailModelling the probability and impact of false-positive serology for Borrelia burgdorferi sensu lato: A case study
HOUBEN, Rosa; Meersschaert, Carole ULiege; HENDRICKX, Guy et al

in Equine Veterinary Journal (2020)

BACKGROUND:Serological screening tests for lyme borreliosis have poor specificity, with potential for misdiagnosis and unnecessary antimicrobial treatment. OBJECTIVES:To evaluate the impact of lyme ... [more ▼]

BACKGROUND:Serological screening tests for lyme borreliosis have poor specificity, with potential for misdiagnosis and unnecessary antimicrobial treatment. OBJECTIVES:To evaluate the impact of lyme borreliosis seroprevalence and serologic test characteristics on the probability of obtaining a false positive result and impact on antimicrobial use. STUDY DESIGN:Cross-sectional serological survey and modelling. METHODS:Sera from 303 horses in southern Belgium were analysed by enzyme-linked immunosorbent assay (ELISA). Apparent seroprevalence was derived from serological data and a Bayesian estimate of true seroprevalence was computed. These were a starting point to model the impact of test and population characteristics on the probability of obtaining false positive results and consequently unnecessary treatments and complications. RESULTS:Apparent and true seroprevalence were 22% (95% CI 18-27%) and 11% (credible interval with 95% probability 0.6-21%) respectively. We estimate that two-thirds of positive samples are false-positive in southern Belgium, with one in five of tested horses potentially misdiagnosed as infected. Around 5% of antimicrobial use in equine veterinary practice in Belgium may be attributable to treatment of a false positive result. MAIN LIMITATIONS:There was uncertainty regarding the ELISA's sensitivity and specificity. CONCLUSIONS:This study highlights the importance of appreciating the poor diagnostic value of ELISA screening for lyme borreliosis as demonstrated by this case study of seroprevalence in southern Belgium where we demonstrate that a nontrivial number of horses is estimated to receive unwarranted treatment due to poor appreciation of screening test characteristics by practitioners, contributing substantially to unnecessary use of antimicrobials. [less ▲]

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See detailEffect of oral administration of omeprazole on the microbiota of the gastric glandular mucosa and feces of healthy horses
Cerri, Simona; Taminiau, Bernard ULiege; Haché-Carré de Lusancay, Alexandra et al

in Journal of Veterinary Internal Medicine (2020), 34

Background Proton pump inhibitors, such as omeprazole, are used extensively in both human and veterinary patients for treatment and prevention of gastric ulcer syndrome. In human and companion animal ... [more ▼]

Background Proton pump inhibitors, such as omeprazole, are used extensively in both human and veterinary patients for treatment and prevention of gastric ulcer syndrome. In human and companion animal medicine, omeprazole administration has been associated to shifts in gastric and fecal microbiota and increased incidence of Clostridioides difficile enterocolitis, especially in prolonged treatments. Hypothesis/Objectives The purpose of this study was to assess the effect of omeprazole on gastric glandular and fecal microbiota in healthy adult horses. Animals Eight healthy research horses stabled on straw and fed 100% haylage. Methods Prospective controlled study. Transendoscopic gastric glandular biopsies and gastric fluid as well as fresh fecal samples were obtained from each horse at a 7-day interval without treatment to serve as controls. Samples were taken on the same horses before and after a 7-day treatment with omeprazole (4mg/kg PO q24h). Fluid pH was immediately assessed and the rest of samples were kept frozen at -20°C until analysis. Bacterial taxonomy profiling was obtained by V1V3 16S amplicon sequencing from feces and gastric glandular biopsies. Analysis of alpha, beta diversity and comparison between time points were performed with MOTHUR and results were considered significant when p<0.05. Results Significant structural diversity in the microbiota was found in the glandular gastric samples after 7 days of omeprazole administration. No significant differences were found in composition and diversity of fecal microbiota. Conclusions and clinical importance A short course of oral omeprazole does not modify fecal microbiota in healthy horses but modifies the structure of gastric glandular microbiota [less ▲]

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See detailTwo-dimensional speckle tracking echocardiography in goats: Repeatability, variability, and validation of the technique using an exercise test and an experimentally induced acute ischemic cardiomyopathy
Leroux, A. A.; Moonen, Marie ULiege; Farnir, Frédéric ULiege et al

in BMC Veterinary Research (2020), 16(1),

Background: Two-dimensional speckle tracking (2DST) technique has been validated in numerous animal species, but neither studies of repeatability nor measurements after exercise or in animals with cardiac ... [more ▼]

Background: Two-dimensional speckle tracking (2DST) technique has been validated in numerous animal species, but neither studies of repeatability nor measurements after exercise or in animals with cardiac disease have been reported in goats. Goats are an attractive candidate for animal models in human cardiology because they are easy to handle and have a body and heart size comparable to that of humans. Therefore, the aim of this study was to validate this technique in goats for further clinical and experimental applications in this species. Results: This study was divided into several steps. First, a standardized echocardiographic protocol was performed and 5 cineloops of a right parasternal short-axis view at papillary muscles level were recorded three times at one-day intervals in ten healthy adult unsedated Saanen goats to test repeatability and variability of 2DST measurements. Then, the same measurements were performed immediately before and after a standardized exercise on treadmill in seven of the goats, and at 24 h after induction of an experimental ischemic cardiomyopathy in five of the goats, to test the reliability of the technique to assess physiological and pathological changes. Average and regional measurements of radial and circumferential strain and strain rate, radial displacement, rotation and rotation rate were obtained. Comparisons were performed using two-way ANOVA (p < 0.05). Caprine 2DST average measurements have demonstrated a good repeatability with a low to moderate variability for all measurements except for the diastolic peaks of the circumferential strain rate, radial strain rate and rotation rate. Segmental 2DST measurements were less repeatable than average measurements. Time effect of two-way ANOVA was significant for anteroseptal segment diastolic peaks measurements, rotation and rotation rate measurements. Overall variability of segmental measurements was moderate or high. Segmental and average peak values obtained after exercise and after myocardial ischemia were significantly different than curves obtained at baseline. Conclusions: The results of this study are consistent with those previously described in other animal species and humans. 2DST echocardiography is a valid technique to evaluate physiological and pathological changes in myocardial function in goats, despite the technical limitations observed in this species. © 2020 The Author(s). [less ▲]

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See detailLa myopathie atypique : le top 10 des dernières nouvelles
Votion, Dominique ULiege; François, Anne-Christine ULiege; Renaud, Benoît ULiege et al

in Mespoulhès, Céline (Ed.) Proceeding Journée AVEF (Association des Vétérinaires Equins Français) (2019, November 15)

Cet écrit reprend le top 10 des dernières nouvelles relatives à la myopathie atypique dont certaines ne sont pas encore publiées.

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See detailHigh seroprevalence against Lawsonia intracellularis in horses living in Belgium
Loublier, Clémence ULiege; Cerri, Simona; Gryspeerdt, Annick et al

Poster (2019, November 08)

Lawsonia intracellularis (LI) is an obligate intracellular gram-negative rod causing equine proliferative enteropathy (EPE). Occasional cases of EPE in foals have been reported in Belgium, but the ... [more ▼]

Lawsonia intracellularis (LI) is an obligate intracellular gram-negative rod causing equine proliferative enteropathy (EPE). Occasional cases of EPE in foals have been reported in Belgium, but the seroprevalence of equine LI in the country is unknown. The aim of this study was to determine the seroprevalence of LI in adult healthy horses in Belgium. The target population included clinically healthy adult horses, housed in the same premises for the previous 12 months. Serum samples from horses living throughout Belgium were collected and analysed for specific IgG antibodies against LI using a blocking ELISA test. Results were expressed as Percentage of Inhibition (PI). Samples that had a PI <20% were judged as negative, those between 20 and 30% as inconclusive and those >30% were considered positive. A total of 356 blood samples were analysed with 352 horses (98.8%) testing positive, 2 horses (0. 56%) testing negative and 2 horses (0.56%) showing inconclusive results. The high prevalence of positive samples precluded any statistical analysis to test the impact of province of residence, age, breed or gender on the results. In conclusion, the large percentage of seropositive samples obtained in this study confirms a widespread exposure of Belgian horses to LI. Seroconversion is rarely associated to clinical disease, but suggests that EPE should be part of the differential diagnosis in horses with compatible clinical signs (ill-thrift, hypoproteinemia, edema, and diarrhea). These results are in accordance with results of prevalence reported in neighbouring countries (The Netherlands, Germany), whilst other countries (Israel) report a much lower prevalence. [less ▲]

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See detailUlcerative lesion of the external ear canal causing headshaking in a horse
Wimmer-Scherr, Christina ULiege; Evrard, Laurence ULiege; Amory, Hélène ULiege et al

in Veterinary Record Case Reports (2019)

This case report describes the approach to an unusual case of headshaking of 1-year duration paired with unilateral ear hypersensitivity in a 11-year-old Belgian Warmblood mare. After the exclusion of ... [more ▼]

This case report describes the approach to an unusual case of headshaking of 1-year duration paired with unilateral ear hypersensitivity in a 11-year-old Belgian Warmblood mare. After the exclusion of other potential causes, an aural endoscopy was performed under general anaesthesia revealing an ulcerative lesion in the external right ear canal. Biopsies of this area revealed signs of skin inflammation characterised by acanthosis, hyperkeratosis and epidermal oedema leading to a diagnosis of otitis externa. Interestingly, extensive fungal flora characterised by budding yeasts was present in the stratum corneum of each biopsy. Considering the literature debating the difference between physiological fungal colonisation versus pathogenic growth, the horse received topical antifungal and steroid treatment combined with an ear cleanser solution for 12 days with subsequent resolution of most presenting complaints. A mini-review of current knowledge and terminology in human and small animal veterinary medicine concerning external otitis and otomycosis has been included. [less ▲]

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See detailPoints clés de la gestion clinique des cas de myopathie atypique
François, Anne-Christine ULiege; Renaud, Benoît ULiege; Cesarini Latorre, Carlota ULiege et al

in Pratique Vétérinaire Équine (2019), 201

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See detailCardiovascular, respiratory, gastrointestinal and behavioral effects of intravenous lidocaine in healthy, conscious horses and evaluation of their relationship with lidocaine and monoethylglycinexylidide serum concentrations
Salciccia, Alexandra ULiege; Lopez Arevalo, Diego; Maes, An et al

in Vlaams Diergeneeskundig Tijdschrift (2019), 88(2), 67-76

This study aimed to evaluate the relationship between the serum concentrations of lidocaine/ monoethylglycinexylidide (MEGX) and their effects on several systems in horses. Five healthy, conscious horses ... [more ▼]

This study aimed to evaluate the relationship between the serum concentrations of lidocaine/ monoethylglycinexylidide (MEGX) and their effects on several systems in horses. Five healthy, conscious horses received a two-hour placebo intravenous infusion followed by a two-hour lidocaine infusion (bolus of 1.3 mg/kg over ten minutes followed by a continuous rate infusion of 0.05 mg/kg/min). Lidocaine and MEGX serum concentrations were sampled every ten to fifteen minutes during the experiment, and the presence of muscle fasciculations and loss of balance as well as the respiratory, digestive and cardiovascular systems of the five horses were evaluated by means of different non-invasive methods. During the lidocaine infusion, the mean (± SD) lidocaine and MEGX concentrations were respectively 768.88 ± 93.32ng/ml and 163.08 ± 108.98 ng/ml. The infusion of lidocaine significantly influenced the presence of fasciculations, caused a statistically but non-clinically significant decrease of systolic and diastolic blood pressures, which were both correlated with lidocaine and MEGX serum concentrations, and it increased the duodenal contractions frequency, which was correlated with the serum lidocaine concentration. In this study, mild hypotensive and prokinetic effects of short-term lidocaine infusion were observed. [less ▲]

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See detailCorrelation between faecal microbiota and serum levels of Hypoglycin A and MCPA-carnitine in horses with atypical myopathy.
Cesarini Latorre, Carlota ULiege; Votion, Dominique ULiege; BOEMER, François ULiege et al

in 11th Annual European College of Equine Internal Medicine Congress 9‐10 November, 2018. J Vet Intern Med, 33: 1547-1560. (2018, November 09)

Equine atypical myopathy (AM) results from hypoglycin A (HGA) ingestion. Both HGA and its principal metabolite, methylenecyclopropyl acetic acid-carnitine (MCPA-carnitine), are found in serum of horses ... [more ▼]

Equine atypical myopathy (AM) results from hypoglycin A (HGA) ingestion. Both HGA and its principal metabolite, methylenecyclopropyl acetic acid-carnitine (MCPA-carnitine), are found in serum of horses with clinical signs of AM. Not all horses ingesting HGA develop AM, suggesting potential protective factors at horse level. The aim of this study was to assess the correlation between faecal microbiota and serum levels of HGA and MCPA-carnitine in horses with AM. Faecal and serum samples were obtained from 19 horses with a history and clinical signs suggestive of AM. Determination of MCPAcarnitine serum concentrations using tandem mass spectrometry and HGA quantification using a modified aTRAQ® assay helped to confirm the disease. Bacterial taxonomy profiling was obtained by V1 V3 16S amplicon sequencing from faeces. Comparison between both groups was performed with a two-tailed Mann-Whitney test (P < 0.05). Spearman rank correlation between bacterial taxa and HGA and MCPA-carnitine were performed with MOTHUR. A total of 190 000 sequences were analysed and clustered to 296 genus level operational taxonomic units. Serum levels of HGA were positively correlated with the relative abundance of the Prevotellaceae family (rs 0,64) and MCPA-carnitine levels were negatively correlated with the relative abundance of the Lachnospiraceae family (rs−0,66). Previous work had shown a significantly lower relative abundance of the Lachnospiraceae family in AM affected horses when compared to healthy co-grazers. Results of this pilot study suggest that Lachnospiraceae could play a preventative role in the development of clinical disease. The role of intestinal microbiome in the development of AM deserves further investigation. [less ▲]

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See detailEffects of oral administration of omeprazole on equine faecal microbiota
Hache-Carré de Lusancay, Alexandra; Taminiau, Bernard ULiege; Lecoq, Laureline ULiege et al

in Proceedings du FARAH Day (2018)

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See detailDiagnostic de la maladie de Lyme.
Pitel, Pierre-Hugues; Hary, Claudine; Amory, Hélène ULiege et al

Conference (2017, December 14)

Le diagnostic de certitude de la maladie Borreliose de Lyme est éminemment complexe. La seule efficacité thérapeutique n’étant pas une preuve absolue, seule un faisceau de concordance et la réalisation ... [more ▼]

Le diagnostic de certitude de la maladie Borreliose de Lyme est éminemment complexe. La seule efficacité thérapeutique n’étant pas une preuve absolue, seule un faisceau de concordance et la réalisation d’études terrain pourront nous aider à essayer de débroussailler ce qui est encore chez le cheval une nébuleuse sujette à de nombreuses croyances et controverses. Certaines de ces études françaises et belges seront exposées lors de la présentation orale. [less ▲]

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See detailTwo-dimensional speckle tracking echocardiography in calves: feasibility and repeatability study
Lecoq, Laureline ULiege; Moula, Nassim ULiege; Amory, Hélène ULiege et al

in Journal of Veterinary Cardiology (2017)

Objectives: To assess the feasibility and repeatability of two-dimensional speckle tracking echocardiography for the evaluation of left ventricular function in healthy calves. Animals: 12 Holstein calves ... [more ▼]

Objectives: To assess the feasibility and repeatability of two-dimensional speckle tracking echocardiography for the evaluation of left ventricular function in healthy calves. Animals: 12 Holstein calves, 62 ± 11.6 days old; 75.25 ± 5.4 Kg. Methods: Observational study. Right parasternal short-axis views at papillary muscle level were recorded in standing calves and subsequently analysed by two-dimensional speckle tracking for global and regional radial and circumferential strains and strain rates and radial displacement. Echocardiographic examinations were performed by 2 observers to evaluate intra- and interobserver repeatability and variability. Results: Two-dimensional speckle tracking was feasible in all calves. Automated tracking was better in systole than in diastole. Repeatability of the technique was good in calves. Systolic radial strain and strain rate peak values showed little variability compared to systolic circumferential strain and strain rate and to all diastolic measurements. Variability of the interobserver measurements was greater than the intraobserver measurements. Conclusions: Two-dimensional speckle tracking is feasible in calves. As in other species, evaluation of systolic radial left ventricular function is more reliable than circumferential and diastolic left ventricular function. [less ▲]

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See detailComparison of faecal microbiota of horses suffering from atypical myopathy and healthy co-grazers
Cerri, Simona ULiege; Taminiau, Bernard ULiege; Votion, Dominique ULiege et al

Poster (2017, November 02)

Objectives: To characterize faecal microbiota of horses with atypical myopathy (AM) compared with healthy co-grazers (HcG). Methods: Fresh faecal samples were obtained from 6 horses (1 stallion, 3 ... [more ▼]

Objectives: To characterize faecal microbiota of horses with atypical myopathy (AM) compared with healthy co-grazers (HcG). Methods: Fresh faecal samples were obtained from 6 horses (1 stallion, 3 geldings and 2 females; mean age of 11.810 years) with confirmed AM and 6 HcG (4 geldings and 2 females; mean age of 13.68 years) during autumn-2016 and spring-2017 AM outbreaks in Belgium. Bacterial taxonomy profiling obtained by 16S amplicon sequencing of faeces was used to identify differentially distributed bacterial taxa between AM and HcG. Results were statistically compared using Welch's t-test with STAMP software. Results: A total of 90,407 sequences were analysed and clustered to 8,066 operational taxonomic units. Bacterial populations were distributed between 17 phylas, although 20% of sequences could not be attributed to an existing phylum. Horses with AM harboured a significantly higher relative abundance of Ruminococcaeae family with a significantly lower Lachnospiraceae when compared to HcG. Discussion: AM is caused by hypoglycin A intoxication, but only a part of horses pasturing in the same toxic environment develops the pathology, suggesting that there may be protective factors at the horse level. The results of this study show significant differences in faecal microbiota between AM cases and HcG, which could suggest that microbiota could play a role in the development or prevention of clinical disease. Conclusions: Results demonstrate that microbiota of AM affected horses is significantly different compared to HcG. Significance: Microbiome could influence the development of AM, but this role deserves further investigation. [less ▲]

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See detailComparison of faecal microbiota of horses suffering from atypical myopathy and healthy co-grazers.
Cerri, S.; Taminiau, Bernard ULiege; Votion, Dominique ULiege et al

Scientific conference (2017, October 13)

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