References of "Pastoret, Paul-Pierre"
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See detailGlycoprotein B of Bovine Herpesvirus 4 Is a Major Component of the Virion, Unlike That of Two Other Gammaherpesviruses, Epstein-Barr Virus and Murine Gammaherpesvirus 68
Lomonte, P.; Filée, Patrice ULiege; Lyaku, J. R. et al

in Journal of Virology (1997), 71(4), 3332-5

This study reports that in bovine herpesvirus 4, glycoprotein B (gB) is a heterodimer and a major component of the virion, unlike gBs of Epstein-Barr virus (gp110) and murine gammaherpesvirus 68, two ... [more ▼]

This study reports that in bovine herpesvirus 4, glycoprotein B (gB) is a heterodimer and a major component of the virion, unlike gBs of Epstein-Barr virus (gp110) and murine gammaherpesvirus 68, two other gammaherpesviruses. These are new characteristics with regard to the general features of gB in the Gammaherpesvirinae subfamily. [less ▲]

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See detailAntigens
Van Regenmortel, M; Tellam, R; Manteca, C et al

in Pastoret, PP; Blancou, J; Vannier, P (Eds.) et al Veterinary Vaccinology (1997)

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See detailBoth Wild-Type And Strongly Attenuated Bovine Leukemia Viruses Protect Peripheral Blood Mononuclear Cells From Apoptosis
Dequiedt, Franck ULiege; Hanon, Emilien ULiege; Kerkhofs, P. et al

in Journal of Virology (1997), 71(1),

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See detailOptimization of murine CD8+ cytotoxic T-lymphocyte responses to pseudorabies virus
Depierreux, C.; Graff, I.; Lancelot, V. et al

in Journal of Immunological Methods (1997), 203

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See detailEtablissement d'un plan volontaire de contrôle de la rhinotrachéite infectieuse bovine en région wallonne de Belgique
De Wergifosse, Bertrand; Lemaire, Mylène; Pastoret, Paul-Pierre ULiege et al

in Annales de Médecine Vétérinaire (1997), 141

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See detailLe rôle de la vaccination dans la prévention des maladies respiratoires d'origine virale chez les bovins
Thiry, Etienne ULiege; Pastoret, Paul-Pierre ULiege; Dispas, Marc et al

in Les troubles respiratoires des bovins, synthèse à partir de cas concrets (1997)

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See detailLes différents vaccins disponibles contre la rhinotrachéite infectieuse bovine
Thiry, Etienne ULiege; Lemaire, Mylène; Schynts, Frédéric et al

in Bulletin des Groupements Techniques Vétérinaires (1997), 4B

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See detailBovine herpesvirus type 4. In Lymphotropicherpesviruses: Epstein-Barr virus and Human herpesvirus 8
Thiry, Etienne ULiege; Lomonte, P.; Vanderplasschen, Alain ULiege et al

in International Agency for research on cancer (1997)

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See detailSequence Analysis of the Bovine Herpesvirus Type 1 Genes Homologous to the DNA Polymerase (Ul30), the Major DNA-Binding Protein (Ul29) and Icp18.5 Assembly Protein (Ul28) Genes of Herpes Simplex Virus
Meyer, Gilles; Vlcek, C.; Paces, V. et al

in Archives of Virology (1997), 142(1), 89-102

The nucleotide sequence of a 10.5 kb region (map position 0.332 to 0.410) of bovine herpesvirus type 1 (BHV-1) was determined. This region contained three open reading frames (ORFs) homologous to herpes ... [more ▼]

The nucleotide sequence of a 10.5 kb region (map position 0.332 to 0.410) of bovine herpesvirus type 1 (BHV-1) was determined. This region contained three open reading frames (ORFs) homologous to herpes simplex virus DNA polymerase catalytic subunit (DNApol, UL30), major DNA-binding protein (MDBP, UL29) and ICP18.5 assembly protein (ICP18.5, UL28). The BHV-1 DNApol. MDBP and ICP18.5 ORFs were 1246, 1203 and 826 amino acids long with a calculated molecular mass of 134.2 kDa, 124.4 kDa and 86.9 kDa, respectively. They showed a high homology with alphaherpesvirus homologs despite large differences in the G + C content of the UL30-UL28 segment ranging from 44.4% for varicella zoster virus to 71.5% for BHV-1. Particularly well conserved among Alphaherpesvirinae are the putative functional domains of the DNApol and MDBP proteins which are discussed. Phylogenetic analysis revealed that BHV-1 clustered in the Varicellovirus genus with the animal D-type viruses. In this group, the BHV-1 position was shown to vary according to the investigated genes. Indeed, pseudorabies virus clustered with BHV-1 in the DNApol tree but with equine herpesvirus 1 in the ICP18.5 tree. [less ▲]

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See detailBovine Herpesvirus 1-Induced Apoptosis Occurs At The G0/G1 Phase Of The Cell Cycle
Hanon, Emilien ULiege; Hoornaert, S.; Dequiedt, Franck ULiege et al

in Virology (1997), 232(2), 351-358

We have previously shown that bovine herpesvirus 1 (BHV-1), even when inactivated, induces apoptotic cell death in mitogen-stimulated bovine peripheral blood mononuclear cells (PBMCs) (Hanon et al., 1996 ... [more ▼]

We have previously shown that bovine herpesvirus 1 (BHV-1), even when inactivated, induces apoptotic cell death in mitogen-stimulated bovine peripheral blood mononuclear cells (PBMCs) (Hanon et al., 1996, J. Virol. 70, 4116-4120). In order to gain insight into this process, we have investigated the cell cycle phase at which BHV-1 induces apoptosis in PBMCs. Our results show that the percentage of cells that progress through the S phase was always lower in BHV-1-infected PBMCs than in control cells. This effect was not due to a defective activation of mitogen-stimulated PBMCs since BHV-1 only slightly affected the percentage of cells expressing BoCD25, a well-known lymphocyte activation marker. Furthermore, mimosine and cyclosporine A, two chemicals that inhibit entry into the S phase of the cell cycle by different pathways, did not affect the ability of BHV-1 to induce apoptosis. BHV-1-induced apoptosis also occurred in unstimulated PBMCs and interestingly, this was associated with the expression of c-myc and BoCD25 proteins both of which are related to cell cycle progression. All together, these data provide evidence demonstrating that BHV-1-induced apoptosis occurs at the G0/G1 phase of the cell cycle. [less ▲]

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See detailLa rhinotrachéite infectieuse bovine : caractéristiques du virus, l'infection et ses manifestations cliniques
Thiry, Etienne ULiege; Lemaire, Mylène; Vanderheijden, Nathalie et al

in Bulletin des Groupements Techniques Vétérinaires (1997), 568

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See detailThe Role of Glycoproteins gC, gE, gI and gG in the Induction of Cell-Mediated Immune Responses to Bovine Herpesvirus 1
Denis, M.; Hanon, E.; Rijsewijk, F. A. et al

in Veterinary Microbiology (1996), 53(1-2), 121-32

Mutant viruses with deletions in genes encoding non-essential glycoproteins are considered as promising bovine herpesvirus 1 (BHV1) vaccine candidates. The present study compared the influence of various ... [more ▼]

Mutant viruses with deletions in genes encoding non-essential glycoproteins are considered as promising bovine herpesvirus 1 (BHV1) vaccine candidates. The present study compared the influence of various gene deletions (gC, gE, gI, gG) on the induction of cell-mediated immune responses against the virus. The highest BHV1 specific lymphoproliferative response was observed in the group of calves inoculated with the gC- mutant. However, in all groups of inoculated calves, limiting dilution analysis showed marked individual variability in the number of BHV1 specific T lymphocytes that were stimulated. The same animals were then challenged with wild-type BHV1. In these animals, limiting dilution analysis did not reveal gE, gI nor gG as a major T lymphocyte antigen. However, further analysis suggested the T cell antigenicity of gE in a low number of BHV1 hyperimmunized calves. Stimulation of MHC unrestricted cytotoxicity was also evaluated after inoculation with the various deletion mutants. Cytotoxicity in gC- inoculated calves was as high as in BHV1 inoculated calves. In conclusion, among the BHV1 deletion mutants that were tested, the gC- mutant stimulated the best cell-mediated immune responses. [less ▲]

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See detailStructural and Functional Analysis of Bovine Herpesvirus 1 Minor Glycoproteins
Baranowski, Eric; Keil, Günther; Lyaku, Japhet et al

in Veterinary Microbiology (1996), 53(1-2), 91-101

This paper focuses on the structure and functions of bovine herpesvirus 1 minor glycoproteins gH, gE, gG and gp42. It reviews the progress which has been made in their identification and characterization ... [more ▼]

This paper focuses on the structure and functions of bovine herpesvirus 1 minor glycoproteins gH, gE, gG and gp42. It reviews the progress which has been made in their identification and characterization, in the study of their temporal expression and processing in infected cells, and finally in the understanding of their biological activities. In addition, aspects discussed include a comparison with two other alphaherpesviruses, namely herpes simplex virus and pseudorabies virus. [less ▲]

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See detailBovine Herpesvirus 4: Genomic Organization and Relationship with Two Other Gammaherpesviruses, Epstein-Barr Virus and Herpesvirus Saimiri
Lomonte, P.; Bublot, M.; van Santen, V. et al

in Veterinary Microbiology (1996), 53(1-2), 79-89

Bovine herpesvirus 4 (BHV-4) belongs to the gammaherpesvirinae subfamily. Although the whole sequence of BHV-4 genome is not known it was possible, based on random sequencing, to assume that its genomic ... [more ▼]

Bovine herpesvirus 4 (BHV-4) belongs to the gammaherpesvirinae subfamily. Although the whole sequence of BHV-4 genome is not known it was possible, based on random sequencing, to assume that its genomic organization consists of genes clustered in blocks whose orientation and location in the genome are conserved within a herpesvirus subfamily. Between these blocks lie genes which are specific to either a particular virus or a virus subfamily. BHV-4 genome consists of 5 gene blocks conserved among the gammaherpesviruses and particularly within the Epstein-Barr virus (EBV) and the herpesvirus saimiri (HVS) genomes. Analysis of the regions located outside the gene blocks showed the presence of 12 open reading frames (ORFs). Protein database comparisons showed that no ORF translation products were similar to proteins encoded by alpha- or beta-herpesviruses. Nevertheless, 5 ORFs were homologous in amino acid sequences to proteins encoded by HVS and one was similar to a protein encoded by both HVS and EBV. On the basis of the molecular data BHV-4 is more closely related to HVS than to EBV. Genes homologous to cellular genes have been described in both HVS and EBV genomes. No genes homologous to presently sequenced cellular genes were found among those found in the BHV-4 genome to date. [less ▲]

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See detailEstablishment of a Rabbit Model for Bovine Herpesvirus Type 5 Neurological Acute Infection
Meyer, Gilles; Lemaire, Mylène; Lyaku, J. et al

in Veterinary Microbiology (1996), 51(1-2), 27-40

This study was conducted to evaluate the suitability of the rabbit as a model for bovine, herpesvirus 5 (BHV-5) acute infection. In a preliminary experiment, a total of 24 one-month old New Zealand white ... [more ▼]

This study was conducted to evaluate the suitability of the rabbit as a model for bovine, herpesvirus 5 (BHV-5) acute infection. In a preliminary experiment, a total of 24 one-month old New Zealand white rabbits were inoculated with BHV-5 or bovine herpesvirus 1 (BHV-1) by the intraconjunctival, intracerebral or intranasal routes. BHV-5 or BHV-1 inoculated in the conjunctiva induced virus proliferation in the eye mucosae and the nasal cavity of rabbits without meningo-encephalitis. On the other hand, only BHV-5 infection by intranasal or intracerebral routes produced a fatal meningo-encephalitis. The intranasal route was used in a further experiment for the establishment of a rabbit model for BHV-5 infection. A total of 45 rabbits were inoculated intranasally with BHV-5 or BHV-1. The results showed that intranasal inoculation of BHV-5 strain N569 in rabbits was followed by the development of a lethal meningo-encephalitis for 66% of rabbits while all BHV-1 infected rabbits remained healthy throughout this experiment (28 days). Analysis between the mortalities of rabbits infected with BHV-5 and BHV-1 were highly significant (p < 0.001). The presence of BHV-5 in the central nervous system (CNS) was confirmed by virus isolation (essentially the cerebrum, midbrain and pons) and by immunohistochemical staining of BHV-5 antigen (essentially in the neurons of the cerebrum) only in BHV-5 infected rabbits showing clinical signs of meningo-encephalitis. The findings obtained confirmed the suitability of a rabbit model for the establishment of BHV-5 neurological acute infection and also as a valuable tool for the comparative study of BHV-5 and BHV-1 neuropathogenicity. [less ▲]

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See detailInactivated Bovine Herpesvirus 1 Induces Apoptotic Cell Death of Mitogen-Stimulated Bovine Peripheral Blood Mononuclear Cells
Hanon, E.; Vanderplasschen, Alain ULiege; Lyaku, S. et al

in Journal of Virology (1996), 70(6), 4116-4120

Bovine herpesvirus 1 (BHV-1) is able to inhibit the proliferation of bovine peripheral blood mononuclear cells. Here, we have demonstrated that live BHV-1 and, interestingly, inactivated BHV-1 can induce ... [more ▼]

Bovine herpesvirus 1 (BHV-1) is able to inhibit the proliferation of bovine peripheral blood mononuclear cells. Here, we have demonstrated that live BHV-1 and, interestingly, inactivated BHV-1 can induce apoptosis of mitogen-stimulated bovine peripheral blood mononuclear cells in vitro. [less ▲]

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See detailLa traction : un effort impressionnant
Art, Tatiana ULiege; Crigel, M. H.; Vandenput, Sandrina ULiege et al

in Pastoret, Paul-Pierre; Laurant, P.; Courtois, R. (Eds.) et al Le Cheval Ardennais (1996)

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