References of "NISOLLE, Michelle"
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See detailL'utilisation du ratio sFlt-1/PlGF dans la prééclampsie: une analyse rétrospective monocentrique
Verbeurgt, Lievine ULiege; CHANTRAINE, Frédéric ULiege; DE MARCHIN, Jérôme et al

in Revue Médicale de Liège (in press)

La fms-like tyrosine kinase 1 soluble (sFlt-1) est un facteur anti-angiogénique libéré en quantité excessive dans la prééclampsie (PE) et impliqué dans la dysfonction endothéliale. Il est comparé au ... [more ▼]

La fms-like tyrosine kinase 1 soluble (sFlt-1) est un facteur anti-angiogénique libéré en quantité excessive dans la prééclampsie (PE) et impliqué dans la dysfonction endothéliale. Il est comparé au facteur de croissance placentaire pro-angiogénique (PlGF) qui diminue dans la PE. Le ratio sFlt-1/PlGF est présenté dans la littérature comme outil dans la prédiction de la prééclampsie. Un ratio < 38 confirme l’absence de prééclampsie à court-terme. Un ratio ≥ 85 dans la PE précoce (avant 34 semaines aménorrhée (SA)) et ≥ 110 dans le «late onset» (> 34 semaines d’aménorhhée) peut poser le diagnostic de prééclampsie. Dans cette étude rétrospective monocentrique, le ratio sFlt-1/PlGF a été dosé chez 183 patientes à risque dont 67 ont présenté une prééclampsie. Le ratio sFlt-1/PlGF médian pour toutes les patientes évaluées est 100,3. Le ratio médian pour les patientes ayant déclaré une prééclampsie (N=67) est 212,7 alors que celui des femmes sans prééclampsie (N=116) est de 35,4. En accord avec ces analyses, un ratio sFlt-1/PlGF ≥ 38 possède une sensibilité égale à 95,5% et une spécificité égale à 73,3%. Les valeurs prédictives positive (VPP) et négative (VPN) sont, respectivement, 67,4% et 96,6%. Ces résultats suggèrent que le ratio sFlt-1/PlGF peut être une aide dans le diagnostic de la prééclampsie [less ▲]

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See detailLes infections gynécologiques basses
BRICHANT, Géraldine ULiege; TEBACHE, Linda ULiege; NISOLLE, Michelle ULiege

Conference (2017, November 25)

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See detailESTROGEN RECEPTORS AND ESTETROL-DEPENDENT NEUROPROTECTIVE ACTIONS: A PILOT STUDY
Tskitishvili, Ekaterine ULiege; Pequeux, Christel ULiege; Munaut, Carine ULiege et al

Conference (2017, October 20)

Context: Estetrol (E4) has strong antioxidative, neurogenic and angiogenic effects in neural system resulting in the attenuation of neonatal hypoxic–ischemic encephalopathy. Objective: We aimed to define ... [more ▼]

Context: Estetrol (E4) has strong antioxidative, neurogenic and angiogenic effects in neural system resulting in the attenuation of neonatal hypoxic–ischemic encephalopathy. Objective: We aimed to define the role of estrogen receptors in E4-dependent actions in neuronal cell cultures and prove the promyelinating effect of E4. Methods: In vitro the antioxidative and cell survival/proliferating effects of E4 on H2O2-induced oxidative stress in primary hippocampal cell cultures were studied using different combinations of specific inhibitors for ERα (MPP dihydrochloride), ERβ (PHTTP), GPR30 (G15) and palmytoilation (2-BR). LDH activity and cell survival assays were performed. In vivo the promyelinating role of different concentrations of E4 (1 mg/kg/day, 5 mg/kg/day, 10 mg/kg/day, 50 mg/kg/day) was investigated using the hypoxic–ischemic brain damage model in the 7-day-old immature rats before/after the induction of hypoxic–ischemic insult. Myelin basic protein (MBP) immunostaining was performed on brain coronal sections. Results: Our results show that LDH activity is significantly upregulated in cell cultures where the E4’s effect was completely blocked by concomitant treatment either with ERα and ERβ inhibitors (MPP and PHTPP, respectively), or ERα and ERβ inhibitors combined with 2-BR. Cell survival is significantly downregulated in cell cultures where the effect of E4 was blocked by ERβ inhibitor (PHTTP) alone. The blockage of GRP30 receptor did affect neither LDH activity nor cell survival. MBP immunostaining is significantly upregulated in E4-pretreated groups at a concentration of 5 mg/kg/day and 50 mg/kg/day E4, whereas the MBP-positive area OD ratio is significantly increased in all the E4-treated groups. Conclusions: E4’s antioxidative actions mostly depend on ERα and ERβ, whereas neurogenesis and possibly promyelinating activities might be realized through ERβ. [less ▲]

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See detailDrainage vaginal spontané d'un abcès appendiculaire chez une fillette de 7 mois.
BRICHANT, Géraldine ULiege; FRERE, Julie ULiege; El Hachemi, Mounia et al

Poster (2017, October)

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See detailPrise en charge multidisciplinaire de la douleur pelvienne chronique
BRICHANT, Géraldine ULiege; NISOLLE, Michelle ULiege

Conference (2017, August 31)

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See detailNON-INVASIVE ANALYSIS OF POTENTIALLY DYSREGULATED MIRNAS IN ENDOMETRIOSIS
Munaut, Carine ULiege; Emonard, Violaine; TIMMERMANS, Marie ULiege et al

Conference (2017, May 17)

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See detailLes mutilations génitales féminines: la situation à Liège
Swenen, Laurie ULiege; BRICHANT, Géraldine ULiege; Kaluanga, Armand et al

in Revue Médicale de Liège (2017), 72(1), 25-31

En raison des flux migratoires actuels, le praticien obstétricien sera amené à prendre en charge des patientes porteuses de mutilations génitales féminines (MGF). Cet article reprend les principales ... [more ▼]

En raison des flux migratoires actuels, le praticien obstétricien sera amené à prendre en charge des patientes porteuses de mutilations génitales féminines (MGF). Cet article reprend les principales complications obstétricales en relation avec celles-ci. Nous avons comparé un groupe de patientes ayant subi une MGF (n = 69) à un groupe contrôle (n = 162) et étudié les données concernant la patiente, la grossesse en cours, l’accouchement, le nouveau-né, le post-partum et la durée du séjour hospitalier. Dans le groupe MGF, 44 % des patientes sont porteuses de streptocoque B contre 23 % dans le groupe contrôle (p inférieur a 0,01). Concernant le périnée, dans le groupe MGF, une épisiotomie est pratiquée chez 31 % des patientes versus 35 % dans le groupe contrôle; une déchirure survient dans 42 % des accouchements par voie basse dans le groupe MGF contre 26 % dans le groupe contrôle. [less ▲]

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See detailRecommendations for the surgical treatment of endometriosis-part 1: ovarian endometrioma.
Saridogan, Ertan; Becker, Christian M.; Feki, Anis et al

in Gynecological Surgery (2017), 14(1), 27

Study question: What does this document on the surgical treatment of endometriosis jointly prepared by the European Society for Gynaecological Endoscopy (ESGE), ESHRE, and the World Endometriosis Society ... [more ▼]

Study question: What does this document on the surgical treatment of endometriosis jointly prepared by the European Society for Gynaecological Endoscopy (ESGE), ESHRE, and the World Endometriosis Society (WES) provide? Summary answer: This document provides recommendations covering technical aspects of different methods of surgery for endometriomas in women of reproductive age. What is already known: Endometriomas (ovarian endometriotic cysts) are a commonly diagnosed form of endometriosis, owing to the relative ease and accuracy of ultrasound diagnosis. They frequently present a clinical dilemma as to whether and how to treat them when found during imaging or incidentally during surgery. Previously published guidelines have provided recommendations based on the best available evidence, but without technical details on the management of endometriosis. Study design size and duration: A working group of ESGE, ESHRE and WES collaborated on writing recommendations on the practical aspects of endometrioma surgery. Participants/materials setting and methods: This document focused on endometrioma surgery. Further documents in this series will provide recommendations for surgery of deep and peritoneal endometriosis. Main results and the role of chance: The document presents general recommendations for surgery of endometrioma and specific recommendations for cystectomy, ablation by laser or by plasma energy, electrocoagulation and a combination of these techniques applied together or with an interval between them. Limitations and reasons for caution: Owing to the limited evidence available, recommendations are mostly based on clinical expertise. Wider implications of the findings: These recommendations complement previous guidelines on the management of endometriosis. Study funding/competing interests: The meetings of the working group were funded by ESGE, ESHRE and WES. CB declares to be a member of the independent data monitoring committee for a clinical study by ObsEva and receiving research grants from Bayer, Roche Diagnostics, MDNA Life Sciences and Volition. ES received honoraria for provision of training to healthcare professionals from Ethicon, Olympus and Gedeon Richter. The other authors declare that they have no conflict of interest. [less ▲]

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See detailEvaluation retrospective de l'efficacite de l'interruption volontaire de grossesse medicamenteuse au premier trimestre en fonction de l'age gestationnel.
Thiebaut, M.; FIRQUET, Anne ULiege; Pintiaux, Axelle ULiege et al

in Revue Médicale de Liège (2017), 72(4), 193-198

There are two methods of abortion : the medical and the surgical methods, each with its own specific benefits. Theoretically, the limit of medical abortion gestational age is 7 weeks of amenorrhea ... [more ▼]

There are two methods of abortion : the medical and the surgical methods, each with its own specific benefits. Theoretically, the limit of medical abortion gestational age is 7 weeks of amenorrhea. However, since 2011, our university department provides the opportunity of a drug regimen until 9 weeks of amenorrhea. This paper describes the results of a retrospective study which covers 804 patients who were submitted to a medical abortion with gestational ages of 6 to 9 weeks. The purpose is to investigate the efficiency of the medical approach up to 9 weeks and to assess the drug dose required according to the term of pregnancy. Our work shows an efficiency of 95 % of medical abortion up to 9 weeks gestation, with no significant variation with the gestational age. However, maintaining this level of efficiency during the progress of pregnancy requires an increase of the administered doses of misoprostol. In addition, there is a significant increase in the use of analgesics. The medical abortion procedure remains effective until 9 weeks of gestation. This pharmacological approach reduces the risks induced, in the short and long term by the surgical procedure. [less ▲]

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See detailVaginocutaneous fistula and buttock abscess formation 7 years after polypropylene transobturator tape insertion.
ABDALLAH, Alain; NISOLLE, Michelle ULiege; DE LANDSHEERE, Laurent ULiege

in Journal de Gynécologie, Obstétrique et Biologie de la Reproduction (2017), 46

Surgical treatment for stress urinary incontinence (SUI) using transobturator tape insertion is widely accepted. However several postoperative complications were reported in the literature including ... [more ▼]

Surgical treatment for stress urinary incontinence (SUI) using transobturator tape insertion is widely accepted. However several postoperative complications were reported in the literature including infections, abscess and fistula formation. Here we report a case of 57 year old female who presented with abscess and left vaginocutaneous buttock fistula 7 years after transobturator polypropelene tape insertion. Treatment included abscess drainage with dissection of the fistulous tract and removal of the left arm of the transobturator tape along with antibiotic coverage. Sinus drainage stopped after 3 days. Stress urinary incontinence didn't recur. Suspicion of fistula formation should rise in patients presenting with bothersome vaginal or cutaneous discharge after transobturator tape insertion. This case is particular since it describes a fistula complication with polypropelene tape which is unusual with this type of tapes. Treatment of such complication should always consist of surgical removal of the mesh to allow closure of the fistulous tract. [less ▲]

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See detailEstrogen receptors and estetroldependent neuroprotective actions: a pilot study
Tskitishvili, Ekaterine ULiege; Pequeux, Christel ULiege; Munaut, Carine ULiege et al

in Journal of Endocrinology (2017), 232(1), 85-95

Estetrol (E4) has strong antioxidative, neurogenic and angiogenic effects in neural system resulting in the attenuation of neonatal hypoxic-ischemic encephalopathy. We aimed to define the role of estrogen ... [more ▼]

Estetrol (E4) has strong antioxidative, neurogenic and angiogenic effects in neural system resulting in the attenuation of neonatal hypoxic-ischemic encephalopathy. We aimed to define the role of estrogen receptors in E4-dependent actions in neuronal cell cultures and prove the promyelinating effect of E4. In vitro the antioxidative and cell survival/proliferating effects of E4 on H2O2-induced oxidative stress in primary hippocampal cell cultures were studied using different combinations of specific inhibitors for ERalpha (MPP dihydrochloride), ERbeta (PHTTP), GPR30 (G15) and palmytoilation (2-BR). LDH activity and cell survival assays were performed. In vivo the promyelinating role of different concentrations of E4 (1 mg/kg/day, 5 mg/kg/day, 10 mg/kg/day, 50 mg/kg/day) was investigated using the hypoxic-ischemic brain damage model in the 7-day-old immature rats before/after the induction of hypoxic-ischemic insult. Myelin basic protein (MBP) immunostaining was performed on brain coronal sections. Our results show that LDH activity is significantly upregulated in cell cultures where the E4's effect was completely blocked by concomitant treatment either with ERalpha and ERbeta inhibitors (MPP and PHTPP, respectively), or ERalpha and ERbeta inhibitors combined with 2-BR. Cell survival is significantly downregulated in cell cultures where the effect of E4 was blocked by ERbeta inhibitor (PHTTP) alone. The blockage of GRP30 receptor did affect neither LDH activity nor cell survival. MBP immunostaining is significantly upregulated in E4-pretreated groups at a concentration of 5 mg/kg/day and 50 mg/kg/day E4, whereas the MBP-positive area OD ratio is significantly increased in all the E4-treated groups. E4's antioxidative actions mostly depend on ERalpha and ERbeta, whereas neurogenesis and possibly promyelinating activities might be realized through ERbeta. [less ▲]

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See detailDysregulated circulating miRNAs in pre-eclampsia
Tebache, Linda; Munaut, Carine ULiege; Blacher, Silvia ULiege et al

Conference (2016, September 25)

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See detailEstetrol Attenuates Neonatal Hypoxic-Ischemic Encephalopathy: Preclinical Studies
Tskitishvili, Ekaterine ULiege; Nisolle, Michelle ULiege; Noël, Agnès ULiege et al

Poster (2016, June 17)

Brain hypoxia and ischemia due to systemic hypoxemia and reduced cerebral blood flow (CBF) are the primary causes of neonatal hypoxic-ischemic encephalopathy (HIE) accompanied by gray and white matter ... [more ▼]

Brain hypoxia and ischemia due to systemic hypoxemia and reduced cerebral blood flow (CBF) are the primary causes of neonatal hypoxic-ischemic encephalopathy (HIE) accompanied by gray and white matter injuries occurring in neonates. Perinatal HIE still remains a challenge in perinatal medicine. About 20% of affected newborns die in the postnatal period, and an additional 25% will sustain childhood disabilities. So far no medical treatment provides important neuroprotection against HIE. Studies of new neuroprotective agents in animal models of HIE may have importance for the development of new compounds and treatment strategies for this pathological condition. Estetrol (E4) is a recently described estrogen with four hydroxyl-groups that is synthesized exclusively during pregnancy by the human fetal liver. It has important antioxidative activity. To study the neuroprotective and therapeutic effects of E4 in vivo neonatal HIE model of 7-day-old newborn rat pups was used. Rat pups body temperatures were examined along with their body and brain weights. Brains were studied at the level of the hippocampus and cortex. Intact cell counting and expressions of markers for neuronal cell viability (microtubule-associated protein-2 (MAP-2)), neurogenesis (doublecortin (DCX)) and angiogenesis (vascular-endothelial growth factor (VEGF)) were evaluated by histo- and immunohistochemistry. The serum levels of brain damage markers (S100B and glial fibrillary acidic protein (GFAP)) were measured by ELISA. Our results demonstrate for the first time that E4 has a significant neuroprotective and therapeutic effects. It decreases the early gray matter loss and promotes neuro- and angiogenesis in vivo. Estetrol treatment has no effects on body weight, brain weight or body temperature. Taken together, E4 might become an important safe and physiological substance to treat neonatal HIE. [less ▲]

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See detailUse of Estetrol with other Steroids for Attenuation of Neonatal Hypoxic-Ischemic brain injury:to combine or not to combine?
Tskitishvili, Ekaterine ULiege; Pequeux, Christel ULiege; Munaut, Carine ULiege et al

in Oncotarget (2016)

Estetrol (E4), estradiol (E2) and progesterone (P4) have important antioxidative and neuroprotective effects in neuronal system. We aimed to study the consequence of combined steroid therapy in neonatal ... [more ▼]

Estetrol (E4), estradiol (E2) and progesterone (P4) have important antioxidative and neuroprotective effects in neuronal system. We aimed to study the consequence of combined steroid therapy in neonatal hypoxic-ischemic encephalopathy (HIE). In vitro the effect of E4 combined with other steroids on oxidative stress and the cell viability in primary hippocampal cultures was evaluated by lactate dehydrogenase and cell survival assays. In vivo neuroprotective and therapeutic efficacy of E4 combined with other steroids was studied in HIE model of immature rats. The rat pups rectal temperature, body and brain weights were evaluated. The hippocampus and the cortex were investigated by histo/immunohistochemistry: intact cell number counting, expressions of markers for early gray matter lose, neuro- and angiogenesis were studied. Glial fibrillary acidic protein was evaluated by ELISA in blood samples. In vitro E4 and combinations of high doses of E4 with P4 and/or E2 significantly diminished the LDH activity and upregulated the cell survival. In vivo pretreatment or treatment by different combinations of E4 with other steroids had unalike effects on body and brain weight, neuro- and angiogenesis, and GFAP expression in blood. The combined use of E4 with other steroids has no benefit over the single use of E4. [less ▲]

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See detailEndométriose minime et légère: quel impact sur la fertilité?
BRICHANT, Géraldine ULiege; AUDEBERT, Alain; NISOLLE, Michelle ULiege

in Revue Médicale de Liège (2016), 71(5), 236-241

L’endométriose minime ou légère (stades I/II) est fréquemment identifiée chez les femmes hypofertiles, surtout en cas de stérilité inexpliquée. L’impact de ces lésions sur la fertilité est controversé ... [more ▼]

L’endométriose minime ou légère (stades I/II) est fréquemment identifiée chez les femmes hypofertiles, surtout en cas de stérilité inexpliquée. L’impact de ces lésions sur la fertilité est controversé, certains les considérant comme paraphysiologiques. Elles sont hétérogènes dans leur étendue, leur activité biologique, l’inflammation qui les caractérise ou les adhérences qu’elles peuvent induire. La diminution de la qualité ovocytaire, et de la mobilité des spermatozoïdes ainsi que les altérations endométriales apparaissent comme les mécanismes les plus pertinents pour expliquer l’hypofertilité. La fécondité spontanée des femmes présentant une endométriose minime ou légère est diminuée en comparaison avec celle des femmes pour lesquelles le diagnostic de stérilité inexpliquée a été posé. L’insémination intra-utérine avec stimulation de l’ovulation améliore la fertilité des femmes traitées. L’ablation coelioscopique des lésions endométriosiques améliore de façon modeste la fécondité, conduisant à recommander cette pratique compte tenu de ses faibles risques. La fécondation in vitro (FIV) est le moyen le plus efficace permettant d’obtenir des résultats similaires ou légèrement inférieurs à ceux observés chez les femmes témoins présentant une infertilité tubaire. L’impact des endométrioses minimes et légères sur la fertilité apparaît très probable, au moins pour certaines lésions qui demandent à être identifiées. [less ▲]

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See detailNeonatal Hypoxic-Ischemic Encephalopathy: a new view of an old problem
Tskitishvili, Ekaterine ULiege; VIELLEVOYE, Renaud ULiege; Gérard, Céline et al

in Références en Gynécologie Obstétrique (2016), 17(1-1),

Neonatal hypoxic-ischemic encephalopathy (HIE) remains a challenge of perinatal medicine. It is an important cause of long term morbidity, including motor and behavioral deficits, mental retardation ... [more ▼]

Neonatal hypoxic-ischemic encephalopathy (HIE) remains a challenge of perinatal medicine. It is an important cause of long term morbidity, including motor and behavioral deficits, mental retardation, seizures and cerebral palsy, and mortality in newborns. This paper reviews the pathophysiology and current concepts of the management of neonatal HIE as well as the future potential neuroprotective strategies for attenuation of this disease. [less ▲]

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