References of "Evrard, Brigitte"
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Dupuis, Henri; Léonard, Grégoire ULiege; Detrembleur, Christophe ULiege et al

Article for general public (2019)

Le dioxyde de carbone (CO2) est devenu l’ennemi à abattre. Les chercheurs de la plateforme FRITCO2T, qui réunit quatre laboratoires, essaient au contraire de le valoriser

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See detailAssessment of the feasibility to develop a fast and easy reproducible 3D bronchial model growing at the air-liquid interface: Which critical culture parameters must be controlled?
Lechanteur, Anna ULiege; Evrard, Brigitte ULiege; Piel, Géraldine ULiege

in European Journal of Pharmaceutics and Biopharmaceutics (2019)

The discovery and evaluation of new active drugs will be easier thanks to the development of 3D-epithelial model mimicking biological barriers. These models close to in vivo conditions should help to ... [more ▼]

The discovery and evaluation of new active drugs will be easier thanks to the development of 3D-epithelial model mimicking biological barriers. These models close to in vivo conditions should help to speed up the drug development process while reducing in vivo animal testing. Regarding lung administration, despite many studies exploring the development of 2D-model using Calu-3 cells, there are still multiple disparities about experimental methods and a standardization is needed. We investigated the impact of different culture parameters (time, cell densities, Transwell™ membrane pore sizes or the culture media) on the integrity and the morphology of a 2D model. Using permeability studies, electron microscopy and immunohistochemistry, we propose an easy and reproducible 2D-model with Calu-3 cells fully differentiated after 14 days of growing at the air-liquid interface. Moreover, based on these results, we went further with a 3D-bronchial model which consists of a collagen matrix, fibroblasts laid under the epithelial layer. We aim to assess the feasibility to develop a 3D cell-based model of the human airway growing at the air-liquid interface. Many parameters which have to be tightly controlled to develop an extracellular matrix equivalent without any shrinking of the gel encompassing alive fibroblasts as well as over epithelial cells have been identified. Overall, this in vitro model is a differentiated pseudo-stratified bronchial mucosa with mucus expression which is the basis for further optimization allowing the screening of pulmonary drugs in terms of permeability, cytotoxicity or particle-mucus interactions. [less ▲]

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See detailSilicones in development of a highly persistent sprayable emulsion containing essential oils for treatment of common skin infections
Sounouvou, Axel Gérald Hope Tognidé ULiege; Defourny, Charline; Quetin-Leclercq, Joëlle et al

Poster (2019, September)

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See detailOzone-primed neutrophils promote early steps of tumor cell metastasis to lungs by enhancing their NET production
Rocks, Natacha ULiege; Vanwinge, Céline ULiege; Radermecker, Coraline ULiege et al

in Thorax (2019), 0

Air pollution, including particulates and gazes such as ozone (O3), is detrimental for patient’s health and has repeatedly been correlated to increased morbidity and mortality in industrialized countries ... [more ▼]

Air pollution, including particulates and gazes such as ozone (O3), is detrimental for patient’s health and has repeatedly been correlated to increased morbidity and mortality in industrialized countries. Although studies have described a link between ambient particulate matter and increased lung cancer morbidity, no direct relation has yet been established between O3 exposure and metastatic dissemination to lungs. [less ▲]

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See detailAssessment of drug skin tolerance in Africans using a chromameter
Sounouvou, Axel Gérald Hope Tognidé ULiege; Quetin-Leclercq, Joëlle; Piel, Géraldine ULiege et al

Conference (2019, May 20)

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See detailDevelopment of new manufacturing processes for amorphous solid dispersions
Jennotte, Olivier ULiege; Koch, Nathan ULiege; Rocks, Natacha ULiege et al

Conference (2019, May 20)

Use of the hot-melt extrusion form amorphous solid dispersions of atorvastatin calcium trihydrate with a selection of three polymers in order to enhance its aqueous solubility

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See detailHot melt extrusion as a solvent-free technique for the formation of a polymeric amorphous solid dispersion of atorvastatin
Jennotte, Olivier ULiege; Koch, Nathan ULiege; Collard, Laurence ULiege et al

Poster (2019, March 26)

Hot-melt extrusion as a solvent-free technique for the enhancement of BCSII molecules.

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See detailAssessment of drug skin tolerance in Africans using chromameter
Sounouvou, Axel Gérald Hope Tognidé ULiege; Quetin-Leclercq, Joëlle; Piel, Géraldine ULiege et al

Poster (2019, March 26)

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See detailEnhancement of skin penetration of lipid-based nanocarriers
Bellefroid, Coralie ULiege; Lechanteur, Anna ULiege; Evrard, Brigitte ULiege et al

Conference (2019, March 25)

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See detailLipid gene nanocarriers for the treatment of skin diseases: current state-of-the-art
Bellefroid, Coralie ULiege; Lechanteur, Anna ULiege; Evrard, Brigitte ULiege et al

in European Journal of Pharmaceutics and Biopharmaceutics (2019)

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See detailUpdate of the progresses and development prospects of the FEDER project Phare
Emonts, Paul ULiege; Penoy, Noémie ULiege; Rocks, Natacha ULiege et al

Scientific conference (2019, January 19)

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See detailSupercritical fluid methods: an alternative to conventional methods to prepare liposomes
Bigazzi, William ULiege; Penoy, Noémie ULiege; Evrard, Brigitte ULiege et al

in Chemical Engineering Journal (2019)

Liposomes are used as nanovectors to encapsulate several active molecules with a prolongation of the drug effect, to target specific tissues or to reduce certain side effects. Innovative therapies using ... [more ▼]

Liposomes are used as nanovectors to encapsulate several active molecules with a prolongation of the drug effect, to target specific tissues or to reduce certain side effects. Innovative therapies using nanovectors have been developed and accepted for clinical trials, however their conventional methods of production suffer from drawbacks such as the use of large amounts of organic solvents, the small quantities produced and the many steps involved in the production that prevent the production at industrial scale. For several years, supercritical fluid-based preparation processes have been considered as a good alternative to conventional liposome preparation methods. A number of supercritical fluid processes allowing the liposome preparation are described depending on the role of the supercritical fluid with respect to the liposome raw materials. The supercritical fluid can act as a solvent or a cosolvent for the lipids material which are next put in contact with an aqueous solution before or during the depressurization. The supercritical fluid can also be used as an antisolvent to induce the formation of proliposomes which can be easily converted into liposomes by hydration. Finally the supercritical fluid can be used as a dispersing agent in order to finely disperse the lipid molecules into an aqueous medium. Several of these methods using supercritical fluids to produce liposomes are described in the literature. The aim of this review article is to summarize and classify the current supercritical fluid-based liposome preparation methods according to the function of the supercritical fluid within the process and compare them in terms of liposome’s physicochemical properties, hydration methods, molecules that can be encapsulated, encapsulation efficiencies and the use or not of organic solvents. [less ▲]

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See detailDevelopment of injectable liposomes and drug-in-cyclodextrin-in-liposome formulations encapsulating estetrol to prevent cerebral ischemia of premature babies.
Palazzo, Claudio ULiege; Laloy, Julie; Delvigne, Anne Sophie et al

in European Journal of Pharmaceutical Sciences (2019), 9(127), 52-59

Neonatal Hypoxic-Ischemic Encephalopathy (HIE), a brain disease due to brain hypoxia along with ischemia and reduced cerebral blood flow, is one of the primary reasons of severe injury among babies ... [more ▼]

Neonatal Hypoxic-Ischemic Encephalopathy (HIE), a brain disease due to brain hypoxia along with ischemia and reduced cerebral blood flow, is one of the primary reasons of severe injury among babies prematurely born. No efficacy treatment is available to the present day. Estetrol (E4), a major estradiol metabolite, has an important role in the brain development and protection. The aim of this study is to develop new injectable liposome and drug-in-cyclodextrin-in-liposome (DCL) formulations, encapsulating E4 in order to enhance its crossing through the blood-brain barrier (BBB). Liposome and DCL formulations were prepared and were physiochemically characterized. Stability in foetal bovine serum (FBS) was evaluated. LDH and MTS tests on endothelial, neuronal and BBB model cells, as well as hemocompatibility of the nanovectors were performed in vitro. In vitro BBB passage was evaluated using human BBB cell line (hCMEC/D3). All the formulations had average particle size below 150 nm, polydispersity index below 0.10 and ζ potential around + 30 mV. The encapsulation efficacy for liposomes was between 3% and 10% while those of DCL are between 15% and 35%. The effect of liposome and DCL formulations on cell viability and integrity was evaluated. The results showed no toxic effects on all the tested cell lines. Hemocompatibility tests showed no hemolysis, platelet aggregation or effects on coagulation, confirming the possibility of the formulations to be intravenously administrated. BBB passage tests highlighted the capability of the formulations to pass the BBB and reach the brain. Therefore, the formulations are promising drug delivery system to target estrogens to the brain, due to their physiochemical characteristics. [less ▲]

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See detailLiposome production by innovative method using supercritical fluids
Penoy, Noémie ULiege; Bigazzi, William ULiege; Piel, Géraldine ULiege et al

Scientific conference (2018, December 19)

Detailed reference viewed: 59 (25 ULiège)
See detailEnhancement of skin penetration of lipid-based nanocarriers
Bellefroid, Coralie ULiege; Lechanteur, Anna ULiege; Evrard, Brigitte ULiege et al

Scientific conference (2018, December 19)

Detailed reference viewed: 36 (11 ULiège)