References of "Desmecht, Daniel"
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See detailA case of splenic myelolipoma in a German Shepherd dog
Dernier, Adrienne ULiege; Bayrou, Calixte ULiege; Desmecht, Daniel ULiege et al

Poster (2017, October 13)

An older neutered female German Shepherd dog was referred to our Department for necropsic evaluation after euthanasia for chronic renal insufficiency. Apart from chronic renal lesions and gastrointestinal ... [more ▼]

An older neutered female German Shepherd dog was referred to our Department for necropsic evaluation after euthanasia for chronic renal insufficiency. Apart from chronic renal lesions and gastrointestinal ulcerations, the macroscopic examination showed multiple off-white multifocal splenic nodules. Histologic examination revealed multiple, well-demarcated, unencapsulated masses of hematopoietic elements admixed with mature adipose tissue, consistent with a diagnosis of myelolipoma. This benign tumor is rarely reported in dogs in the literature and is more often observed in the form of a single encapsulated nodule. The other possible sites where these tumors can be seen are adrenal glands and organs retaining hematopoietic activity. Although several hypotheses have been proposed, the exact origin of myelolipoma still remain unknown [less ▲]

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See detailClinical and haematological alterations in foot and mouth disease virus naturally-infected domestic water buffaloes in Vietnam
Dam Van Phai, ULiege; BUI TRAN ANH, Dao, ; Desmecht, Daniel ULiege

Poster (2017, October 13)

Foot and mouth disease (FMD) has been one of the most important epizootic diseases in several cloven-hoofed animals including buffaloes, causing severe economic losses in Vietnam. This study aimed at ... [more ▼]

Foot and mouth disease (FMD) has been one of the most important epizootic diseases in several cloven-hoofed animals including buffaloes, causing severe economic losses in Vietnam. This study aimed at observing clinical indicators, physiological parameters, haematological and blood chemistry values of 30 buffaloes naturally infected with FMD virus (FMDV). The study was undertaken from January 2015 to February 2016 in Hanoi, Vietnam. Results show that buffaloes infected with FMDV displayed significantly higher fever, increased respiratory rate and increased heart frequency compared to healthy animals. Moreover, FMDV-infected animals always showed high fever and vesicular stomatitis, often showed edging nail congestive inflammation and, sometimes only, blisters on the nipple. Haematological indicators showed that FMDV-infected buffaloes were dehydrated (increased total protein). Besides, infected animals also underwent anaemia as mean corpuscular haemoglobin and mean corpuscular haemoglobin concentrations were plummeted. Total white blood cell count plummeted too, especially the number and proportion of neutrophils. Meanwhile, the number and proportion of lymphocytes sharply increased compared to healthy animals. Infected buffaloes also displayed signs of liver and heart damages as judged by an increase in concentration of AST and ALT, while there was not renal damage since creatinine concentration remained stable [less ▲]

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See detailATYPICAL DICTYOCAULUS VIVIPAROUS-ASSOCIATED BOVINE ACUTE PNEUMONITIS: REMINISCENT OF LARVAL HYPOBIOSIS DURING THE WINTER?
Bayrou, Calixte ULiege; Dernier, Adrienne ULiege; Cassart, Dominique ULiege et al

Conference (2017, October)

Dictyocaulus viviparus is the aetiological agent of verminous bronchitis in cattle. Herein we describe an atypical case of parasitic pneumonitis in an adult cow. Methods At the end of March 2017, a 3-yr ... [more ▼]

Dictyocaulus viviparus is the aetiological agent of verminous bronchitis in cattle. Herein we describe an atypical case of parasitic pneumonitis in an adult cow. Methods At the end of March 2017, a 3-yr old Belgian Blue cow suddenly displayed a marked respiratory distress syndrome a few hours after C-section calving. In spite of the treatment set up by the local veterinarian, the cow died 48 hours later. The body was referred to the Veterinary Faculty of Liège (Belgium) for necropsy. Post- mortem gross examination revealed a severe, acute interstitial pneumonitis with concurrent emphysema and a significant amount of parasites (Dictyocaulus viviparus) in the bronchi. The microscopic examination confirmed the severe acute interstitial pneumonitis with foci of hemorrhages, necrosis, hyaline membranes and hyperplasia of type II pneumocytes. Larvae were also present in the alveoli and bronchioles. Results Altogether, theses clinical observations and lesions were compatible with a hypersensitivity reaction against worm antigens. Additionally, Baermann’s test was performed on feces collected from the cadaver and from 5 others cows kept under the same conditions. Four out of 6 cows were excreting small amounts of Dictyocaulus stage-1 larvae. Conclusions Parasitic pneumonia is a very common disease in first year grazing young cattle. In this case, cow’s age and disease manifestation time were very uncommon. Three-year-old cows are supposed to be fully protected against this nematode species. Moreover, in Belgium, at the end of March, livestock is still kept indoors (zero grazing winter period). Therefore, this case could highlight the hypobiotic capacity of Dictyocaulus viviparus larvae and associated « periparturient rise » phenomenon, both well known in sheep, but not described in cattle so far. We herein discuss the farming conditions that promoted such a severe parasitic respiratory disease. [less ▲]

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See detailCryopreservation of chicken primordial germ cells by vitrification and slow-freezing: a comparative study
Tonus, Céline ULiege; Connan, Delphine ULiege; Waroux, Olivier ULiege et al

in Theriogenology (2017), 88

In the present study, we compare a classical slow freezing method and an aseptic vitrification technique to cryopreserve a stable Primordial Gem Cells (PGCs) line issued from the Ardennaise chicken breed ... [more ▼]

In the present study, we compare a classical slow freezing method and an aseptic vitrification technique to cryopreserve a stable Primordial Gem Cells (PGCs) line issued from the Ardennaise chicken breed. Viability immediately after warming was close to 80% and did not differ between the two cryopreservation methods. Proliferation tended to be slower for both cryopreservation methods compared to controls, but the difference was significant only for vitrification. No difference was found between the two methods after flow cytometry analysis of SSEA-1 expression and RT-PCR on several factors related to PGCs phenotype. After one week in culture, all cryopreserved cells reached controls main morphological and expanding (viability/proliferation) features. However, slow freezing generated more unwanted cells clusters than vitrification. After injection of the PGCs into recipient embryos, vitrified PGCs showed a clear, yet not significant, tendency to colonize the gonad at a higher rate than slow frozen PGCs. Slow freezing in cryovials remains simple, inexpensive and less technically demanding than vitrification. Nevertheless, the intrinsic advantages of our aseptic vitrification method and the present study suggest that this should be considered as safer than classical slow freezing for cryopreserving chicken PGCs. [less ▲]

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See detailUsutu virus, Belgium, 2016
Garigliany, Mutien-Marie ULiege; Linden, Annick ULiege; Gilliaux, Gautier ULiege et al

in Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases (2017), 48(1), 116-119

During late summer 2016, in a northwest European region extending over Belgium, the Netherlands and the eastern border of the German state of North Rhine Westphalia, an outbreak of wild bird deaths ... [more ▼]

During late summer 2016, in a northwest European region extending over Belgium, the Netherlands and the eastern border of the German state of North Rhine Westphalia, an outbreak of wild bird deaths occurred similar to those reported on the continent since 1996. Dead birds were necropsied and examined by complementary methods. Pathologic and immunohistological investigations strongly suggested an infection by Usutu virus. Subsequently, genomic segments of the said virus were detected, the virus was isolated and its complete genome was sequenced. The strain, designated Usutu-LIEGE, is a close phylogenetic relative of those isolated in Germany which form a distinct group within the USUV phylogeny, the so-called Europe_3 lineage. Should this outbreak recapitulate the characteristics of those in southwest Germany in 2011 and in/around Vienna (Austria) in 2001, it is expected that specific avian populations in the affected area will face a significant reduction in size for a few years. [less ▲]

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See detailA gammaherpesvirus provides protection against allergic asthma by inducing the replacement of resident alveolar macrophages with regulatory monocytes.
Machiels, Bénédicte ULiege; Dourcy, Mickael ULiege; Xiao, Xue ULiege et al

in Nature Immunology (2017)

The hygiene hypothesis postulates that the recent increase in allergic diseases such as asthma and hay fever observed in Western countries is linked to reduced exposure to childhood infections. Here we ... [more ▼]

The hygiene hypothesis postulates that the recent increase in allergic diseases such as asthma and hay fever observed in Western countries is linked to reduced exposure to childhood infections. Here we investigated how infection with a gammaherpesvirus affected the subsequent development of allergic asthma. We found that murid herpesvirus 4 (MuHV-4) inhibited the development of house dust mite (HDM)-induced experimental asthma by modulating lung innate immune cells. Specifically, infection with MuHV-4 caused the replacement of resident alveolar macrophages (AMs) by monocytes with regulatory functions. Monocyte-derived AMs blocked the ability of dendritic cells to trigger a HDM-specific response by the TH2 subset of helper T cells. Our results indicate that replacement of embryonic AMs by regulatory monocytes is a major mechanism underlying the long-term training of lung immunity after infection. [less ▲]

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See detailMoku Virus in Invasive Asian Hornets, Belgium, 2016.
Garigliany, Mutien-Marie ULiege; Taminiau, Bernard ULiege; El Agrebi, Noémie ULiege et al

in Emerging Infectious Diseases (2017), 23(12), 2109-2112

We report the detection of Moku virus in invasive Asian hornets (Vespa velutina nigrithorax) in Belgium. This constitutes an unexpected report of this iflavirus outside Hawaii, USA, where it was recently ... [more ▼]

We report the detection of Moku virus in invasive Asian hornets (Vespa velutina nigrithorax) in Belgium. This constitutes an unexpected report of this iflavirus outside Hawaii, USA, where it was recently described in social wasps. Although virulence of Moku virus is unknown, its potential spread raises concern for European honeybee populations. [less ▲]

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See detailWidespread activity of multiple lineages of Usutu virus, western Europe, 2016.
Cadar, Daniel; Luhken, Renke; van der Jeugd, Henk et al

in Euro Surveillance : Bulletin Européen sur les Maladies Transmissibles = European Communicable Disease Bulletin (2017), 22(4),

In the summer of 2016, Belgium, France, Germany and the Netherlands reported widespread Usutu virus (USUV) activity based on live and dead bird surveillance. The causative USUV strains represented four ... [more ▼]

In the summer of 2016, Belgium, France, Germany and the Netherlands reported widespread Usutu virus (USUV) activity based on live and dead bird surveillance. The causative USUV strains represented four lineages, of which two putative novel lineages were most likely recently introduced into Germany and spread to other western European countries. The spatial extent of the outbreak area corresponded with R0 values > 1. The occurrence of the outbreak, the largest USUV epizootic registered so far in Europe, allowed us to gain insight in how a recently introduced arbovirus with potential public health implications can spread and become a resident pathogen in a naive environment. Understanding the ecological and epidemiological factors that drive the emergence or re-emergence of USUV is critical to develop and implement timely surveillance strategies for adequate preventive and control measures. Public health authorities, blood transfusion services and clinicians in countries where USUV was detected should be aware of the risk of possible USUV infection in humans, including in patients with unexplained encephalitis or other neurological impairments, especially during late summer when mosquito densities peak. [less ▲]

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See detailCell cycle S phase markers are expressed in cerebral neuron nuclei of cats infected by the Feline Panleukopenia Virus
Poncelet, Luc; Garigliany, Mutien-Marie ULiege; Ando et al

in Cell Cycle (Georgetown, Tex.) (2016), 15(24), 3482-3489

The cell cycle-associated neuronal death hypothesis, which has been proposed as a common mechanism for most neurodegenerative diseases, is notably supported by evidencing cell cycle effectors in neurons ... [more ▼]

The cell cycle-associated neuronal death hypothesis, which has been proposed as a common mechanism for most neurodegenerative diseases, is notably supported by evidencing cell cycle effectors in neurons. However, in naturally occurring nervous system diseases, these markers are not expressed in neuron nuclei but in cytoplasmic compartments. In other respects, the Feline Panleukopenia Virus (FPV) is able to complete its cycle in mature brain neurons in the feline species. As a parvovirus, the FPV is strictly dependent on its host cell reaching the cell cycle S phase to start its multiplication. In this retrospective study on the whole brain of 12 cats with naturally-occurring, FPV-associated cerebellar atrophy, VP2 capsid protein expression was detected by immunostaining not only in some brain neuronal nuclei but also in neuronal cytoplasm in 2 cats, suggesting that viral mRNA translation was still occurring. In these cats, double immunostainings demonstrated the expression of cell cycle S phase markers cyclin A, cdk2 and PCNA in neuronal nuclei. Parvoviruses are able to maintain their host cells in S phase by triggering the DNA damage response. S139 phospho H2A1, a key player in the cell cycle arrest, was detected in some neuronal nuclei, supporting that infected neurons were also blocked into the S phase. PCR studies did not support a co-infection with an adeno or herpes virus. ERK1/2 nuclear accumulation was observed in some neurons suggesting that the ERK signaling pathway might be involved as a mechanism driving these neurons far into the cell cycle. [less ▲]

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See detailSoluble forms of CD46 are detected in Bos taurus plasma and neutralize BVDV, the bovine pestivirus
Alzamel, Nidal ULiege; Bayrou, Calixte ULiege; Decreux, Annabelle et al

in Comparative Immunology, Microbiology and Infectious Diseases (2016), 49

The pestivirus bovine viral diarrhea virus (BVDV) is known to bind to the CD46 molecule, which subsequently promotes entry of the virus. Mapping of the BVD-virion-binding site has shown that two peptides ... [more ▼]

The pestivirus bovine viral diarrhea virus (BVDV) is known to bind to the CD46 molecule, which subsequently promotes entry of the virus. Mapping of the BVD-virion-binding site has shown that two peptides, 66EQIV69 and 82GQVLAL87, located on antiparallel beta sheets in the most distal complement control protein module (CCP1), provide the attachment platform. In the present study, we reveal new CD46-encoding transcripts that are predicted to encode CCP1-containing soluble forms. Further, we show that the serum of most adult cattle contains soluble CD46 (sCD46) and that a recombinant soluble isoform neutralizes BVDV infectivity in an in vitro assay. We have then established an ELISA for determination of plasma sCD46 in a large cohort of animals. Overall, serum sCD46 amounts to 8 ± 18 ng/mL (mean ± SD, n = 440), with a IC [95-105] ranging from 6,4 to 9,8 ng/mL and extreme values between 0 and 178 ng/mL. We found that sCD46 is not detectable in fetal and neonatal sera and that its plasma concentration increases progressively up to adulthood. We also detected high- and low-sCD46 performers and show that this phenotype does not depend of environment. As modern rearing techniques make it possible to disseminate genetically-determined phenotypes very quickly in a population, a large-scale study examining whether high-sCD46 animals provide epidemiological protection against BVDV infection and transmission should be undertaken. [less ▲]

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See detailRe-emergence of the Schmallenberg virus associated triad hydranencephaly-micromyelia-arthrogryposis in a newborn calf in Belgium, 2016
Bayrou, Calixte ULiege; Garigliany, Mutien-Marie ULiege; Cassart, Dominique ULiege et al

in Veterinary Record Case Reports (2016), 4(1), 000342

Schmallenberg virus (SBV) emerged in Germany in 2011, then spread rapidly across Europe, causing an epizootic outbreak of abortion, stillbirth and birth at term of lambs, kids and calves with neurological ... [more ▼]

Schmallenberg virus (SBV) emerged in Germany in 2011, then spread rapidly across Europe, causing an epizootic outbreak of abortion, stillbirth and birth at term of lambs, kids and calves with neurological signs and/or musculo-skeletal malformations. SBV-associated disease in newborns disappeared in Belgium in 2013. Here, we describe a SBV genomic RNA-positive malformed calf born in May 2016. It reveals the return of SBV circulation during the fall 2015 in the said area. [less ▲]

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See detailLong term-cultured and cryopreserved primordial germ cells from various chicken breeds retain high proliferative potential and gonadal colonisation competency
Tonus, Céline ULiege; Cloquette, Karine; Ectors, Fabien ULiege et al

in Reproduction, Fertility and Development (2016), 28(5), 628-639

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