PMID- 7957242
OWN - NLM
STAT- MEDLINE
DA  - 19941228
DCOM- 19941228
LR  - 20070723
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 226
IP  - 1
DP  - 1994 Nov 15
TI  - A common system controls the induction of very different genes. The class-A
      beta-lactamase of Proteus vulgaris and the enterobacterial class-C
      beta-lactamase.
PG  - 149-57
AB  - Among the Enterobacteriaceae, Proteus vulgaris is exceptional in the inducible
      production of a 29-kDa beta-lactamase (cefuroximase) with an unusually high
      activity towards the beta-lactamase-stable oximino-cephalosporins (e.g.
      cefuroxime and cefotaxime). Sequencing of the corresponding gene, cumA, showed
      that the derived CumA beta-lactamase belonged to the molecular class A. The
      structural gene was under the direct control of gene cumR, which was transcribed 
      backwards and whose initiation codon was 165 bp away from that of the
      beta-lactamase gene. This resembled the arrangement of structural and regulator
      genes ampC and ampR of the 39-kDa molecular-class-C beta-lactamase AmpC present
      in many enterobacteria. Moreover, cloned genes ampD and ampG for negative
      modulation and signal transduction of AmpC beta-lactamase induction,
      respectively, were also able to restore constitutively CumA overproducing and
      non-inducible P. vulgaris mutants to the inducible, wild-type phenotype. The
      results indicate that controls of the induction phenomena are equivalent for the 
      CumA and AmpC beta-lactamase. Very different structural genes can thus be under
      the control of identical systems.
AD  - Laboratoire d'Enzymologie, Universite de Liege, Sart Tilman, Belgium.
FAU - Datz, M
AU  - Datz M
FAU - Joris, B
AU  - Joris B
FAU - Azab, E A
AU  - Azab EA
FAU - Galleni, M
AU  - Galleni M
FAU - Van Beeumen, J
AU  - Van Beeumen J
FAU - Frere, J M
AU  - Frere JM
FAU - Martin, H H
AU  - Martin HH
LA  - eng
SI  - GENBANK/X80128
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - GERMANY
TA  - Eur J Biochem
JT  - European journal of biochemistry / FEBS
JID - 0107600
RN  - 0 (DNA, Bacterial)
RN  - EC 3.5.2.6 (beta-Lactamases)
SB  - IM
GS  - ampD
GS  - ampG
GS  - cumA
GS  - cumR
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Bacterial
MH  - Enzyme Induction
MH  - Escherichia coli/genetics
MH  - Genes, Bacterial
MH  - Genetic Complementation Test
MH  - Molecular Sequence Data
MH  - Proteus vulgaris/*enzymology/genetics
MH  - Sequence Homology, Nucleic Acid
MH  - Signal Transduction
MH  - Transcription, Genetic
MH  - beta-Lactamases/*biosynthesis/genetics/metabolism
EDAT- 1994/11/15
MHDA- 1994/11/15 00:01
CRDT- 1994/11/15 00:00
PST - ppublish
SO  - Eur J Biochem. 1994 Nov 15;226(1):149-57.