PMID- 8471035
OWN - NLM
STAT- MEDLINE
DA  - 19930512
DCOM- 19930512
LR  - 20091118
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 291 ( Pt 1)
DP  - 1993 Apr 1
TI  - An overview of the kinetic parameters of class B beta-lactamases.
PG  - 151-5
AB  - The catalytic properties of three class B beta-lactamases (from Pseudomonas
      maltophilia, Aeromonas hydrophila and Bacillus cereus) were studied and compared 
      with those of the Bacteroides fragilis enzyme. The A. hydrophila beta-lactamase
      exhibited a unique specificity profile and could be considered as a rather
      specific 'carbapenemase'. No relationships were found between sequence
      similarities and catalytic properties. The problem of the repartition of class B 
      beta-lactamases into sub-classes is discussed. Improved purification methods were
      devised for the P. maltophilia and A. hydrophila beta-lactamases including, for
      the latter enzyme, a very efficient affinity chromatography step on a
      Zn(2+)-chelate column.
AD  - Universita degli Studi dell'Aquila, Dipartimento di Scienze e Tecnologie
      Biomediche e di Biometria, Italy.
FAU - Felici, A
AU  - Felici A
FAU - Amicosante, G
AU  - Amicosante G
FAU - Oratore, A
AU  - Oratore A
FAU - Strom, R
AU  - Strom R
FAU - Ledent, P
AU  - Ledent P
FAU - Joris, B
AU  - Joris B
FAU - Fanuel, L
AU  - Fanuel L
FAU - Frere, J M
AU  - Frere JM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - ENGLAND
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - EC 3.5.2.6 (beta-Lactamases)
SB  - IM
MH  - Aeromonas hydrophila/enzymology
MH  - Bacillus cereus/enzymology
MH  - Catalysis
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Isoelectric Point
MH  - Kinetics
MH  - Molecular Weight
MH  - Pseudomonas/enzymology
MH  - beta-Lactamases/chemistry/isolation & purification/*metabolism
PMC - PMC1132494
OID - NLM: PMC1132494
EDAT- 1993/04/01
MHDA- 1993/04/01 00:01
CRDT- 1993/04/01 00:00
PST - ppublish
SO  - Biochem J. 1993 Apr 1;291 ( Pt 1):151-5.